Ilex paraguariensis cell suspension culture characterization and response against ethanol

Plant Cell, Tissue and Organ Culture - Tập 68 - Trang 257-263 - 2002
Kátia H. Kraemer1,2, Eloir P. Schenkel3, Robert Verpoorte2
1Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre-RS, Brazil
2Division of Pharmacognosy, Gorlaeus Laboratories, LACDR, Leiden University, Leiden, The Netherlands
3Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

Tóm tắt

Cell suspension cultures of Ilex paraguariensis, a South American native tree known as the maté plant, were initiated in order to investigate plant defense. Cultures were characterized for their cell growth, chemical composition and sugar consumption. The present work quantified some effects of salicylic acid, methyl jasmonate, cellulase and ethanol on cell growth and sugar metabolism. Results suggest that salicylic acid and methyl jasmonate play a role in Ilex paraguariensis response against exogenous ethanol by enhancing its glucosylation by 50% if compared to addition of ethanol alone.

Tài liệu tham khảo

Astarita LV (1996) Cultura in vitro de massas celulares suspensor-embrionárias de Araucaria angustifolia: Morfogênese celular e poliembriogênese somática. UFGRS, Porto Alegre. (MSc-dissertation) Correa MP (1969) Dicionário das plantas Úteis do Brasil e das exóticas cultivadas, Vol. 4. IBDF, Rio de Janeiro Garcia Paula RD De (1962) Contribuição ao estudo dos alcalóides do mate. Rev. Bras. Quím. 54: 492–494 Doireau P (1976) Importance et divers aspects de la fermentation alcoolique au cours de la germination de différents types de semences. Physiol. Veg. 14: 467–486 Fett-Neto AG, Zhang WY & Dicosmo F (1994) Kinetics of taxol production, growth, and nutrient uptake in cell suspensions of Taxus cuspidata. Biotechnol. Bioeng. 44: 205–210 Gosmann G, Guillaume D, Taketa ATC & Schenkel EP (1995) Triterpenoid saponins from Ilex paraguariensis. J. Nat. Prod. 58: 438–441 Gosmann G, Schenkel EP & Seligmann O (1989) A new saponin from mate, Ilex paraguariensis. J. Nat. Prod. 52: 1367–1370 Kraemer KH, Taketa ACT, Schenkel EP, Gosmann g & Guillaume D (1996) Matesaponin 5, a highly polar saponin from Ilex paraguariensis. Phytochemistry. 42: 1119–1122 Kraemer KH (1997) Ilex paraguariensis St.-Hil. (erva-mate): distribuição de saponinas e estudos iniciais em culturas de células em suspensão. UFRGS, Porto Alegre. (PhD-thesis) Kraemer KH, Schenkel EP & Verpoorte R (1999) Glucosylation of ethanol in Ilex paraguariensis cell suspension cultures. Plant Cell Rep. 18: 509–513 Mendive JR (1940) The occurrence of α-amyrin and ursolic acid in the leaves of Ilex paraguariensis. J. Org. Chem. 5: 235–237 Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497 Pirt SJ (1975) In: Principles of Microbe and Cell Cultivation (pp 66–68). Wiley, New York Rech SB, Batista CVF, Scripsema J, Verpoorte R & Henriques AT (1998) Cell cultures of Rauwolfia sellowii: Growth and alkaloid production. Plant Cell Tiss. Org. Cult. 54: 61–63 Roberts EAH (1956) The chlorogenic acids of tea and maté. Chem. Ind. 37: 985–986 Schenkel EP, Montanha JA & Gosmann G (1996) Triterpene saponins from maté, Ilex paraguariensis. In: Waller G & Yamasaki K (eds) Saponins used in Food and Agriculture (pp 47–56). Plenum Press, New York Schripsema J, Erkelens C & Verpoorte R (1991) Intra-and extra-cellular carbohydrates in plant cell cultures investigated by 1H NMR. Plant Cell Rep. 9: 527–530 Schripsema J & Verpoorte R (1991) Investigation of extracts of plant cell cultures by proton nuclear magnetic resonance spectroscopy. Phytochem. Anal. 2: 155–162 Van der Heijden R, Verheij ER, Schripsema J, Baerheim Svendsen A, Verpoorte R & Harkes PAA (1988) Induction of triterpene biosynthesis by elicitors in suspension cultures of Tabernaemontana species. Plant Cell Rep. 7: 51–54 Wijnsma R, Go JTKA, Van Weerden IN, Harkes PAA, Verpoorte R & Baerheim Svendsen A (1985) Anthraquinones as phytoalexins in cell and tissue cultures of Cinchona species. Plant Cell Rep. 4: 241–244