Identification of bottlenecks in Escherichia coli engineered for the production of CoQ10

Alexander, 1978, Alternative hydroxylases for the aerobic and anaerobic biosynthesis of ubiquinone in Escherichia coli, Biochemistry, 17, 4750, 10.1021/bi00615a024

Baba, 2006, Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection, Mol. Syst. Biol., 2, 10.1038/msb4100050

Bader, 2000, Disulfide bonds are generated by quinone reduction, J. Biol. Chem., 275, 26082, 10.1074/jbc.M003850200

Barker, 2001, Microbial synthesis of p-hydroxybenzoic acid from glucose, Biotechnol. Bioeng., 76, 376, 10.1002/bit.10160

Choi, 2005, Restricted electron flux increases coenzyme Q10 production in Agrobacterium tumefaciens ATCC4452, Process Biochem., 40, 3225, 10.1016/j.procbio.2005.03.038

Choi, 2009, Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q(10) production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene, J. Biotechnol., 144, 64, 10.1016/j.jbiotec.2009.04.010

Cox, 1969, Biosynthesis of ubiquinone in Escherichia coli K-12: location of genes affecting the metabolism of 3-octaprenyl-4-hydroxybenzoic acid and 2-octaprenylphenol, J. Bacteriol., 99, 450, 10.1128/JB.99.2.450-458.1969

Datsenko, 2000, One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products, Proc. Natl. Acad. Sci. USA, 97, 6640, 10.1073/pnas.120163297

Davis, 1951, Inhibition of Escherichia coli by p-aminobenzoic acid and its reversal by p-hydroxybenzoic acid, J. Exp. Med., 94, 243, 10.1084/jem.94.3.243

Echtay, 2000, Coenzyme Q is an obligatory cofactor for uncoupling protein function, Nature, 408, 609, 10.1038/35046114

Geu-Flores, 2007, USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products, Nucleic Acids Res., 35, e55, 10.1093/nar/gkm106

Gin, 2005, Genetic evidence for a multi-subunit complex in coenzyme Q biosynthesis in yeast and the role of the Coq1 hexaprenyl diphosphate synthase, J. Biol. Chem., 280, 2676, 10.1074/jbc.M411527200

Gin, 2003, The Saccharomyces cerevisiae COQ6 gene encodes a mitochondrial flavin-dependent monooxygenase required for coenzyme Q biosynthesis, J. Biol. Chem., 278, 25308, 10.1074/jbc.M303234200

Gu, 2006, Kinetics of Agrobacterium tumefaciens ubiquinone-10 batch production, Process Biochem., 41, 1908, 10.1016/j.procbio.2006.04.002

Ha, 2009, Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens, Bioprocess Biosyst. Eng., 32, 697, 10.1007/s00449-009-0318-9

Ha, 2007, Controlling the sucrose concentration increases coenzyme Q10 production in fed-batch culture of Agrobacterium tumefaciens, Appl. Microbiol. Biotechnol., 76, 109, 10.1007/s00253-007-0995-8

Ha, 2007, Optimization of culture conditions and scale-up to pilot and plant scales for coenzyme Q(10) production by Agrobacterium tumefaciens, Appl. Microbiol. Biotechnol., 74, 974, 10.1007/s00253-006-0744-4

Hamilton, 1971, Ubiquinone biosynthesis in Escherichia coli K-12. Accumulation of an octaprenol, farnesylfarnesylgeraniol, by a multiple aromatic auxotroph, Biochem. J., 123, 435, 10.1042/bj1230435

Harker, 1999, Expression of prokaryotic 1-deoxy-d-xylulose-5-phosphatases in Escherichia coli increases carotenoid and ubiquinone biosynthesis, FEBS Lett., 448, 115, 10.1016/S0014-5793(99)00360-9

Hodgson, 2002, Coenzyme Q10 improves blood pressure and glycaemic control: a controlled trial in subjects with type 2 diabetes, Eur. J. Clin. Nutr., 56, 1137, 10.1038/sj.ejcn.1601464

Hsieh, 2007, Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex, Arch. Biochem. Biophys., 463, 19, 10.1016/j.abb.2007.02.016

Hsu, 2000, Genetic evidence for a multi-subunit complex in the O-methyltransferase steps of coenzyme Q biosynthesis, Biochim. Biophys. Acta, 1484, 287, 10.1016/S1388-1981(00)00019-6

Hsu, 1996, Complementation of coq3 mutant yeast by mitochondrial targeting of the Escherichia coli UbiG polypeptide: evidence that UbiG catalyzes both O-methylation steps in ubiquinone biosynthesis, Biochemistry, 35, 9797, 10.1021/bi9602932

Ingledew, 1984, The respiratory chains of Escherichia coli, Microbiol. Rev., 48, 222, 10.1128/MMBR.48.3.222-271.1984

Inui, 2008, Mechanisms of inhibitory effects of CoQ10 on UVB-induced wrinkle formation in vitro and in vivo, Biofactors, 32, 237, 10.1002/biof.5520320128

Johnson, 2005, COQ9, a new gene required for the biosynthesis of coenzyme Q in Saccharomyces cerevisiae, J. Biol. Chem., 280, 31397, 10.1074/jbc.M503277200

Kien, 2010, Coenzyme Q10 production in a 150-l reactor by a mutant strain of Rhodobacter sphaeroides, J. Ind. Microbiol. Biotechnol., 37, 521, 10.1007/s10295-010-0699-4

Kim, 2006, Amplification of 1-deoxy-d-xyluose 5-phosphate (DXP) synthase level increases coenzyme Q10 production in recombinant Escherichia coli, Appl. Microbiol. Biotechnol., 72, 982, 10.1007/s00253-006-0359-9

Knoell, 1979, Isolation of a soluble enzyme complex comprising the ubiquinone-8 synthesis apparatus from the cytoplasmic membrane of Escherichia coli, Biochem. Biophys. Res. Commun., 91, 919, 10.1016/0006-291X(79)91967-3

Knoell, 1981, Stand-by position of the dioxygen-dependent ubiquinone-8 synthesis apparatus in anaerobically grown Escherichia coli K-12, FEMS Microbiol. Lett., 10, 59, 10.1111/j.1574-6968.1981.tb06206.x

Kovach, 1995, Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes, Gene, 166, 175, 10.1016/0378-1119(95)00584-1

Kroger, 1973, Further evidence for the pool function of ubiquinone as derived from the inhibition of the electron transport by antimycin, Eur. J. Biochem., 39, 313, 10.1111/j.1432-1033.1973.tb03129.x

Lass, 1998, Electron transport-linked ubiquinone-dependent recycling of alpha-tocopherol inhibits autooxidation of mitochondrial membranes, Arch. Biochem. Biophys., 352, 229, 10.1006/abbi.1997.0606

Lawrence, 1974, Biosynthesis of ubiquinone in Escherichia coli K-12: biochemical and genetic characterization of a mutant unable to convert chorismate into 4-hydroxybenzoate, J. Bacteriol., 118, 41, 10.1128/JB.118.1.41-45.1974

Lee, 2004, Cloning and functional expression of the dps gene encoding decaprenyl diphosphate synthase from Agrobacterium tumefaciens, Biotechnol. Prog., 20, 51, 10.1021/bp034213e

Ma, 2011, Optimization of a heterologous mevalonate pathway through the use of variant HMG-CoA reductases, Metab. Eng., 13, 588, 10.1016/j.ymben.2011.07.001

Marbois, 2010, para-aminobenzoic acid is a precursor in coenzyme Q6 biosynthesis in Saccharomyces cerevisiae, J. Biol. Chem., 285, 27827, 10.1074/jbc.M110.151894

Martin, 2007, Coenzyme Q and protein/lipid oxidation in a BSE-infected transgenic mouse model, Free Radical Biol. Med., 42, 1723, 10.1016/j.freeradbiomed.2007.03.005

Martin, 2003, Engineering a mevalonate pathway in Escherichia coli for production of terpenoids, Nat. Biotechnol., 21, 796, 10.1038/nbt833

Martinez-Morales, 1999, Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction, J. Bacteriol., 181, 7143, 10.1128/JB.181.22.7143-7148.1999

Matsumura, 1983, Anaerobic production of ubiquinone-10 by Paracoccus denitrificans, Appl. Microbiol. Biotechnol., 17, 85, 10.1007/BF00499856

Nakajima, 2009, Microbial conversion of glucose to a novel chemical building block, 2-pyrone-4,6-dicarboxylic acid, Metab. Eng., 11, 213, 10.1016/j.ymben.2009.02.002

Ohara, 2004, Engineering of ubiquinone biosynthesis using the yeast coq2 gene confers oxidative stress tolerance in transgenic tobacco, Plant J., 40, 734, 10.1111/j.1365-313X.2004.02246.x

Okada, 1998, Molecular cloning and mutational analysis of the ddsA gene encoding decaprenyl diphosphate synthase from Gluconobacter suboxydans, Eur. J. Biochem., 255, 52, 10.1046/j.1432-1327.1998.2550052.x

Okada, 1997, Cloning of the sdsA gene encoding solanesyl diphosphate synthase from Rhodobacter capsulatus and its functional expression in Escherichia coli and Saccharomyces cerevisiae, J. Bacteriol., 179, 5992, 10.1128/jb.179.19.5992-5998.1997

Okada, 1997, The ispB gene encoding octaprenyl diphosphate synthase is essential for growth of Escherichia coli, J. Bacteriol., 179, 3058, 10.1128/jb.179.9.3058-3060.1997

Okada, 1996, Polyprenyl diphosphate synthase essentially defines the length of the side chain of ubiquinone, Biochim. Biophys. Acta, 1302, 217, 10.1016/0005-2760(96)00064-1

Park, 2005, Batch and fed-batch production of coenzyme Q10 in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans, Appl. Microbiol. Biotechnol., 67, 192, 10.1007/s00253-004-1743-y

Pitera, 2007, Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli, Metab. Eng., 9, 193, 10.1016/j.ymben.2006.11.002

Poon, 2000, Identification of Escherichia coli ubiB, a gene required for the first monooxygenase step in ubiquinone biosynthesis, J. Bacteriol., 182, 5139, 10.1128/JB.182.18.5139-5146.2000

Ringquist, 1992, Translation initiation in Escherichia coli: sequences within the ribosome-binding site, Mol. Microbiol., 6, 1219, 10.1111/j.1365-2958.1992.tb01561.x

Sakato, 1992, Agitation-aeration studies on coenzyme Q10 production using Rhodopseudomonas spheroides, Biotechnol. Appl. Biochem., 16, 19

Sambrook, 1989

Schmelzer, 2007, Functional connections and pathways of coenzyme Q10-inducible genes: an in-silico study, IUBMB Life, 59, 628, 10.1080/15216540701545991

Schmelzer, 2010, The reduced form of coenzyme Q10 mediates distinct effects on cholesterol metabolism at the transcriptional and metabolite level in SAMP1 mice, IUBMB Life, 62, 812, 10.1002/iub.388

Shults, 2002, Effects of coenzyme Q10 in early Parkinson disease: evidence of slowing of the functional decline, Arch. Neurol., 59, 1541, 10.1001/archneur.59.10.1541

Singh, 1998, Randomized, double-blind placebo-controlled trial of coenzyme Q10 in patients with acute myocardial infarction, Cardiovasc. Drugs Ther., 12, 347, 10.1023/A:1007764616025

Suzuki, 1994, Evidence that Escherichia coli ubiA product is a functional homolog of yeast COQ2, and the regulation of ubiA gene expression, Biosci. Biotechnol. Biochem., 58, 1814, 10.1271/bbb.58.1814

Takahashi, 2003, Isolation and expression of Paracoccus denitrificans decaprenyl diphosphate synthase gene for production of ubiquinone-10 in Escherichia coli, Biochem. Eng. J., 16, 183, 10.1016/S1369-703X(03)00035-4

Thomason, 2007, E. coli genome manipulation by P1 transduction, Curr. Protoc. Mol. Biol., 10.1002/0471142727.mb0117s79

Tran, 2006, Complementation of Saccharomyces cerevisiae coq7 mutants by mitochondrial targeting of the Escherichia coli UbiF polypeptide: two functions of yeast Coq7 polypeptide in coenzyme Q biosynthesis, J. Biol. Chem., 281, 16401, 10.1074/jbc.M513267200

Van Dyk, 2004, Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?, J. Bacteriol., 186, 7196, 10.1128/JB.186.21.7196-7204.2004

Wagner, 2006, Rationalizing membrane protein overexpression, Trends Biotechnol., 24, 364, 10.1016/j.tibtech.2006.06.008

Wang, 2000, Isoprenyl diphosphate synthases, Biochim. Biophys. Acta., 1529, 33, 10.1016/S1388-1981(00)00136-0

Weaver, 1990, Cloning of an aroF allele encoding a tyrosine-insensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase, J. Bacteriol., 172, 6581, 10.1128/jb.172.11.6581-6584.1990

Wissenbach, 1990, The specific functions of menaquinone and demethylmenaquinone in anaerobic respiration with fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate by Escherichia coli, Arch. Microbiol., 154, 60, 10.1007/BF00249179

Wu, 2003, Effects of dissolved oxygen concentration and DO-stat feeding strategy on CoQ10 production with Rhizobium radiobacter, World J. Microbiol. Biotechnol., 19, 925, 10.1023/B:WIBI.0000007322.19802.57

Yang, 2010, Coenzyme Q10 reduces beta-amyloid plaque in an APP/PS1 transgenic mouse model of Alzheimer's disease, J. Mol. Neurosci., 41, 110, 10.1007/s12031-009-9297-1

Yen, 2010, Cultivation of Rhodobacter sphaeroides in the stirred bioreactor with different feeding strategies for CoQ(10) production, Appl. Biochem. Biotechnol., 160, 1441, 10.1007/s12010-009-8576-1

Yen, 2009, Coenzyme Q10 production by Rhodobacter sphaeroides in stirred tank and in airlift bioreactor, Bioprocess Biosyst. Eng., 32, 711, 10.1007/s00449-008-0294-5

Yoshida, 1998, Production of ubiquinone-10 using bacteria, J. Gen. Appl. Microbiol., 44, 19, 10.2323/jgam.44.19

Yoshida, 2006, Evaluation of the dietary effects of coenzyme Q in vivo by the oxidative stress marker, hydroxyoctadecadienoic acid and its stereoisomer ratio, Biochim. Biophys. Acta, 1760, 1558, 10.1016/j.bbagen.2006.06.007

Young, 1973, Pathway for ubiquinone biosynthesis in Escherichia coli K-12: gene-enzyme relationships and intermediates, J. Bacteriol., 114, 42, 10.1128/JB.114.1.42-52.1973

Zahiri, 2006, Biochemical characterization of the decaprenyl diphosphate synthase of Rhodobacter sphaeroides for coenzyme Q(10) production, Appl. Microbiol. Biotechnol., 73, 796, 10.1007/s00253-006-0524-1

Zahiri, 2006, Coenzyme Q10 production in recombinant Escherichia coli strains engineered with a heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathway, Metab. Eng., 8, 406, 10.1016/j.ymben.2006.05.002

Zhang, 2007, Ubiquinone-10 production using Agrobacterium tumefaciens dps gene in Escherichia coli by coexpression system, Mol. Biotechnol., 35, 1, 10.1385/MB:35:1:1

Zhang, 2006, Phenotypes and fed-batch fermentation of ubiquinone-overproducing fission yeast using ppt1 gene, J. Biotechnol., 128, 120, 10.1016/j.jbiotec.2006.09.012

Zhu, 1995, Production of ubiquinone in Escherichia coli by expression of various genes responsible for ubiquinone biosynthesis, J. Biosci. Bioeng., 79, 493