Identification of a SulP-type bicarbonate transporter in marine cyanobacteria

Proceedings of the National Academy of Sciences of the United States of America - Tập 101 Số 52 - Trang 18228-18233 - 2004
G. Dean Price1, Fiona J. Woodger1, Murray R. Badger1, Susan M. Howitt1, Loraine Tucker1
1Molecular Plant Physiology Group, Research School of Biological Sciences, and School of Biochemistry and Molecular Biology, Science Faculty, Australian National University, P.O. Box 475, Canberra ACT 0200, Australia

Tóm tắt

Cyanobacteria possess a highly effective CO 2 -concentrating mechanism that elevates CO 2 concentrations around the primary carboxylase, Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). This CO 2 -concentrating mechanism incorporates light-dependent, active uptake systems for CO 2 and HCO – 3 . Through mutant studies in a coastal marine cyanobacterium, Synechococcus sp. strain PCC7002, we identified bicA as a gene that encodes a class of HCO – 3 transporter with relatively low transport affinity, but high flux rate. BicA is widely represented in genomes of oceanic cyanobacteria and belongs to a large family of eukaryotic and prokaryotic transporters presently annotated as sulfate transporters or permeases in many bacteria (SulP family). Further gain-of-function experiments in the freshwater cyanobacterium Synechococcus PCC7942 revealed that bicA expression alone is sufficient to confer a Na + -dependent, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{HCO}}_{3}^{-}\end{equation*}\end{document} uptake activity. We identified and characterized three cyanobacterial BicA transporters in this manner, including one from the ecologically important oceanic strain, Synechococcus WH8102. This study presents functional data concerning prokaryotic members of the SulP transporter family and represents a previously uncharacterized transport function for the family. The discovery of BicA has significant implications for understanding the important contribution of oceanic strains of cyanobacteria to global CO 2 sequestration processes.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 101 Số 52

18228-18233

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