Identification of Novel Variants of High Molecular Weight Glutenin Subunits in Indian Bread Wheat Landraces
Tóm tắt
The High Molecular Weight Glutenin Subunits (HMW GS) in bread wheat landraces were studied using the SDS-PAGE technique. Out of the 32 landraces, 23 were homogeneous while nine showed heterogeneity with respect to the HMW glutenin subunits. Novel variants were observed in HMW GS in four of the landraces. One novel subunit coded by the Glu-B1x locus and two novel subunit pairs at the Glu-D1 locus were identified. A modified system of nomenclature over that of Payne and Lawrence (1983) is suggested for numbering the new subunits. Accordingly, the novel subunits are numbered as 71 (Glu-B1x) and 2+122; 51+122; 52+123 and 53+123(Glu-D1x + y) and the allelic designations are given as Glu-B1 bh for 71+9; Glu-D1bp for 2+122. Glu-D1bq for 51+12; Glu-D1br for 51+122; GluD1br for 52+123 and GluD1bt for 53+123.
Tài liệu tham khảo
Bietz, J.A., Shepherd, K.W., Wall, J.S. 1975. Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem. 52:513–532.
Branlard, G., Dardevet, M. 1985. Diversity of grain proteins and bread wheat quality. I. Correlation between gliadin bands and flour quality characteristics. J. Cereal Sci. 3:329–343.
Gupta, R.K. 2004. HMW glutenin subunits in: T aestivum. In: Gupta, R.K. et al. (eds). DWR Progress Report, 2004, Wheat Quality, AICW&BIP Section, p. 41.
http://shigen.lab.nig.ac.jp/wheat/komugi/genes/download.jsp for HMWGS designations and gene symbols
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteria phage T4. Nature 227:680–685.
Lafiandra, D., Ovidio, R., Porcedu, E., Marigiotta, B. 1993. New data supporting High Mr Glutenin Subunit 5 as determinant of quality differences among the pairs 5+10 and 2+12. J. Cereal Science 18:197–205.
Lagudah, E.S., Halloran, G.M. 1988. Phytogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat. I Variation in HMW subunits of glutenins and gliadins. Theor. Appl. Genet. 75:592–598.
Levy, A.A., Feldman, M. 1988. Ecologeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat Triticum turgidum var. dicoccoides. Theor. Appl. Genet. 75:651–658.
Marigiotta, B., Colaprico, G., D’Ovidio, R., Laflianda, D. 1993. Characterization of high-Mr Subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoresis separations and restriction fragment length polymorphism (RFLP) analyses of their encoding genes. J. Cereal Sci. 17:221–236.
Misra, B.K., Singh, Anju M., Deveshwar, J.J., Pandey, H.N., Ahlawat, A.K. 2005.’ Pissi Local’ an Indian Wheat Germplasm with Unique Quality Features. Abstracts: Poster presented at National Symposium on “Wheat Improvement for the Tropical Areas” held at Coimbatore, September 23–25 P. Full paper in press.
Ng, P.K.W., Bushuk, W. 1988. Statistical relationships between high molecular weight subunits of glutenin and breadmaking quality of Canadian grown wheats. Cereal Chem. 65 (5):408–413.
Ng, P.K.W., Bushuk, W. 1989. Concerning the nomenclature of high molecular weight glutenin subunits. J. Cereal Science 11:53–60.
Payne, P.I., Lawrence, C.J. 1983. Catalogue of alleles for the complex gene loci, Glu A-1, Glu B-1 and Glu D-1, which code for the high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11:29–35.
Payne, P.I., Law, C.N., Mudd, E.E. 1980. Control by homeologous group/chromosomes of high molecular weight subunits of glutenin, a major storage protein of wheat endosperm. Theor. Appl. Genet. 58:113–120.
Payne, P.I., Nightingale, M.A., Kratliger, A.F., Holt, L.M. 1987. The relationship between HMW glutenin subunit composition and the bread making quality of British grown wheat varieties. J. Sci. Good Agric. 40:51–56.
Pogna, N.E., Mellini, F., Beretta, A., Dalbelin Peruffo, A. 1989. The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy. J. Genet and Breed. 43:17–24.
Shewry, P.R., Popinean, Y., Lafiandra, D., Belton, P. 2001. Wheat glutenin subunits and dough elasticity. Trends Food Sci. Technol. 11:433–441.
Shewry, P.R., Napier, J.A., Tatham, A.S. 1995. Seed storage proteins: Structures and biosynthesis. The Plant Cell 7:845–956.
Sreeramulu, G., Singh, N.K. 1994. High molecular weight glutenin subunits of Indian wheat cultivars: Association of subunits 1BL/1RS wheat-rye translocation. J. Cereal Sci. 20:217–225.
Tahir, M., Pavoni, A., Tucci, G.F., Turchetta, T., Lafiandra, D. 1996. Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat. Theor. Appl. Genet. 92:654–659.
Waines, J.G., Payne, P.I. 1987. Electrophoretic analysis of the high molecular weight glutenin subunits of Triticum monococcum. T. urartu and the A genome of bread wheat (Triticum aestivum). Theor. Appl. Genet. 74:71–76.
Wan, Y., Wang, D., Shewry, P.R., Halford, N.G. 2002. Isolation and characterization of five novel high molecular weight subunits of glutenin genes from Triticum timopheevi and Aegilops cylindrical. Theor. Appl. Genet. 104:828–839.
William, M.D.H.M., Pena, R.J., Mujeeb-Kazi, A. 1993. Seed protein and isozyme variations in Triticum tauschii (Aegilops squarrosa). Theor. Appl. Genet. 87:257–263.