Identification and Characterization of the Genes for Mitochondrial Ribosomal Proteins of Saccharomyces Cerevisiae

FEBS Journal - Tập 245 Số 2 - Trang 449-456 - 1997
Madoka Kitakawa1, Hanns‐Rüdiger Graack2, Lutz Grohmann3, Sabine Goldschmidt‐Reisin4, Elke Herfurth5, Brigitte Wittmann‐Liebold5, Taisuke Nishimura1, Katsumi Isono1
1Kobe University, Faculty of Science, Department of Biology, Japan
2Freie Universität Berlin, Institut für Genetik, Germany.
3Institut für Genbiologische Forschung, Berlin, Germany
4Department of Biochemistry, Tel-Aviv University, Israel
5Max‐Delbrück‐Centrum für Molekulare Medizin, AG Proteinchemie, Berlin‐Buch, Germany

Tóm tắt

We have purified 13 large subunit proteins of the mitochondrial ribosome of the yeast Saccharomyces cerevisiae and determined their partial amino acid sequences. To elucidate the structure and function of these proteins, we searched for their genes by comparing our sequence data with those deduced from the genomic nucleotide sequence data of S. cerevisiae and analyzed them. In addition, we searched for the genes encoding proteins whose N‐terminal amino acid sequences we have reported previously [Grohmann, L., Graack, H.‐R., Kruft, V., Choli, T., Goldschmidt‐Reisin, S. & Kitakawa, M. (1991) FEBS Lett. 284, 51–56]. Thus, we were able to identify and characterize 12 new genes for large subunit proteins of the yeast mitochondrial ribosome. Furthermore, we determined the N‐terminal amino acid sequences of seven small subunit proteins and subsequently identified the genes for five of them, three of which were found to be new.

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FEBS Journal

Tập 245 Số 2

449-456

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