Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms

Brem, 2007, Molecular markers in patients with chronic wounds to guide surgical debridement, Mol Med, 13, 30, 10.2119/2006-00054.Brem Sen, 2009, Human skin wounds: a major and snowballing threat to public health and the economy, Wound Repair Regen, 17, 763, 10.1111/j.1524-475X.2009.00543.x Wolcott, 2011, The role of biofilms: are we hitting the right target?, Plast Reconstr Surg, 127, 28S, 10.1097/PRS.0b013e3181fca244 2002 Barrientos, 2008, Growth factors and cytokines in wound healing, Wound Repair Regen, 16, 585, 10.1111/j.1524-475X.2008.00410.x Dhall, 2014, Generating and reversing chronic wounds in diabetic mice by manipulating wound redox parameters, J. Diabetes Res., 562625 Edwards, 2004, Bacteria and wound healing, Curr Opin Infect Dis, 17, 91, 10.1097/00001432-200404000-00004 Flemming, 2007, The EPS matrix: the “house of biofilm cells”, J Bacteriol, 189, 7945, 10.1128/JB.00858-07 Flemming, 2010, The biofilm matrix, Nat Rev Microbiol, 8, 623, 10.1038/nrmicro2415 Lewis, 2005, Persister cells and the riddle of biofilm survival, Biochemistry (Mosc), 70, 267, 10.1007/s10541-005-0111-6 Wolcott, 2015, Disrupting the biofilm matrix improves wound healing outcomes, J Wound Care, 24, 366, 10.12968/jowc.2015.24.8.366 Lewis, 2008, Multidrug tolerance of biofilms and persister cells, Curr Top Microbiol Immunol, 322, 107 Lipsky, 2009, Topical antimicrobial therapy for treating chronic wounds, Clin Infect Dis, 49, 1541, 10.1086/644732 Loo, 2012, Effects of hydrogen peroxide on wound healing in mice in relation to oxidative damage, PloS One, 7, 10.1371/journal.pone.0049215 Pan, 2011, Low levels of hydrogen peroxide stimulate corneal epithelial cell adhesion, migration, and wound healing, Invest Ophthalmol Vis Sci, 52, 1723, 10.1167/iovs.10-5866 Schreml, 2011, A new star on the H2O2rizon of wound healing?, Exp Dermatol, 20, 229, 10.1111/j.1600-0625.2010.01195.x Drosou, 2003, Antiseptics on wounds: an area of controversy, Wounds, 15, 149 Sultana, 2015, Electrochemical scaffold generates localized, low concentration of hydrogen peroxide that inhibits bacterial pathogens and biofilms, Sci Rep, 5, 14908, 10.1038/srep14908 Kiamco, 2019, Hypochlorous-acid-generating electrochemical scaffold for treatment of wound biofilms, Sci Rep, 9, 2683, 10.1038/s41598-019-38968-y Raval, 2019, Hydrogen-peroxide-generating electrochemical scaffold eradicates methicillin-resistant Staphylococcus aureus biofilms, Glob Chall, 3, 1800101, 10.1002/gch2.201800101 Raval, 2020, Hydrogen peroxide-generating electrochemical scaffold activity against trispecies biofilms, Antimicrob Agents Chemother, 64, 10.1128/AAC.02332-19 Mohamed, 2021, Hydrogen peroxide-producing electrochemical bandage controlled by a wearable potentiostat for treatment of wound infections, Biotechnol Bioeng, 118, 2815, 10.1002/bit.27794 Stoffel, 2020, A multimodel regime for evaluating effectiveness of antimicrobial wound care products in microbial biofilms, Wound Repair Regen, 28, 438, 10.1111/wrr.12806 Flurin, 2021, An integrated HOCl-producing e-scaffold is active against monomicrobial and polymicrobial biofilms, Antimicrob Agents Chemother, 65, 10.1128/AAC.02007-20 Cochran, 2000, Reduced susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramine, J Appl Microbiol, 88, 22, 10.1046/j.1365-2672.2000.00825.x James, 2008, Biofilms in chronic wounds, Wound Repair Regen, 16, 37, 10.1111/j.1524-475X.2007.00321.x Wolcott, 2013, The polymicrobial nature of biofilm infection, Clin Microbiol Infect, 19, 107, 10.1111/j.1469-0691.2012.04001.x Bowler, 2001, Wound microbiology and associated approaches to wound management, Clin Microbiol Rev, 14, 244, 10.1128/CMR.14.2.244-269.2001 Sahli, 2016, Microbiology and risk factors associated with war-related wound infections in the Middle East, Epidemiol Infect, 144, 2848, 10.1017/S0950268816000431 Brook, 1998, Aerobic and anaerobic microbiology of chronic venous ulcers, Int J Dermatol, 37, 426, 10.1046/j.1365-4362.1998.00445.x Choi, 2019, Co-occurrence of anaerobes in human chronic wounds, Microb Ecol, 77, 808, 10.1007/s00248-018-1231-z Jernigan, 2020, Multidrug-resistant bacterial infections in US hospitalized patients, 2012–2017, N Engl J Med, 382, 1309, 10.1056/NEJMoa1914433 Serra, 2015, Chronic wound infections: the role of Pseudomonas aeruginosa and Staphylococcus aureus, Expert Rev Anti Infect Ther, 13, 605, 10.1586/14787210.2015.1023291 Mende, 2019, Microbiology of combat-related extremity wounds: trauma infectious disease outcomes study, Diagn Microbiol Infect Dis, 94, 173, 10.1016/j.diagmicrobio.2018.12.008 Roy, 2014, Mixed-species biofilm compromises wound healing by disrupting epidermal barrier function, J Pathol, 233, 331, 10.1002/path.4360 Bessa, 2015, Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection, Int Wound J, 12, 47, 10.1111/iwj.12049 Shirtliff, 2009, Cross-kingdom interactions: Candida albicans and bacteria, FEMS Microbiol Lett, 299, 1, 10.1111/j.1574-6968.2009.01668.x Kean, 2017, Candida albicans mycofilms support Staphylococcus aureus colonization and enhances miconazole resistance in dual-species interactions, Front Microbiol, 8, 258, 10.3389/fmicb.2017.00258 Peters, 2012, Polymicrobial interactions: impact on pathogenesis and human disease, Clin Microbiol Rev, 25, 193, 10.1128/CMR.00013-11 Trizna, 2020, Bidirectional alterations in antibiotics susceptibility in Staphylococcus aureus—Pseudomonas aeruginosa dual-species biofilm, Sci Rep, 10, 1, 10.1038/s41598-020-71834-w del Mar Cendra, 2019, Optimal environmental and culture conditions allow the in vitro coexistence of Pseudomonas aeruginosa and Staphylococcus aureus in stable biofilms, Sci Rep, 9, 1 Townsend, 2017, Implications of antimicrobial combinations in complex wound biofilms containing fungi, Antimicrob Agents Chemother, 61, 10.1128/AAC.00672-17 Linley, 2012, Use of hydrogen peroxide as a biocide: new consideration of its mechanisms of biocidal action, J Antimicrob Chemother, 67, 1589, 10.1093/jac/dks129 McDonnell, 1999, Antiseptics and disinfectants: activity, action, and resistance, Clin Microbiol Rev, 12, 147, 10.1128/CMR.12.1.147 Elkins, 1999, Protective role of catalase in Pseudomonas aeruginosa biofilm resistance to hydrogen peroxide, Appl Environ Microbiol, 65, 4594, 10.1128/AEM.65.10.4594-4600.1999 Raval, 2021, In vitro antibacterial activity of hydrogen peroxide and hypochlorous acid, including that generated by electrochemical scaffolds, Antimicrob Agents Chemother, 65, 10.1128/AAC.01966-20