Hydrodynamic properties of complex, rigid, biological macromolecules: theory and applications

Quarterly Reviews of Biophysics - Tập 14 Số 1 - Trang 81-139 - 1981
José Garcı́a de la Torre1, Victor A. Bloomfield2
1Departamento de Qulmica-FIsica, Faculdad de Ciencias, Universidad de Extremadura, Badajoz, Spain
2Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55108

Tóm tắt

Among the Various methods for characterizing macromolecules in solution, hydrodynamic techniques play a major role. Since the advent of the ultracentrifuge and the development of viscometric apparatus, sedimentation coefficients and intrinsic viscosities have been extensively used to learn about the size and shape of synthetic and biological polymers. More recently, refined techniques such as quasielastic light scattering, transient electric birefringence and fluorescence anisotropy decay have made it possible to obtain in a simple and rapid way quantitative information of high precision on the translational and rotational brownian dynamics of dissolved macromolecules.

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