Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

Springer Science and Business Media LLC - Tập 17 - Trang 376-392 - 2012
Kuo-Liang Yang1,2, Jiunn-Tat Lee3, Cheng-Yoong Pang4,5, Ting-Yi Lee1, Shee-Ping Chen1, Hock-Kean Liew4, Shin-Yuan Chen6, Tzu-Yung Chen7, Py-Yu Lin1
1Buddhist Tzu Chi Stem Cells Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan
2Institute of Medical Biotechnology, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan
3Division of Plastic Surgery, Department of Surgery, Buddhist Tzu Chi Medical Center and Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan
4Department of Research, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan
5Graduate Institute of Clinical Medicine, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan
6Division of Functional Neuroscience, Department of Neurosurgery, Neuro-Medical Scientific Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan
7Department of Neurosurgery, Buddhist Tzu Chi Medical Center Taichung Branch, Buddhist Compassion Relief Tzu Chi Foundation, Taichung, Taiwan

Tóm tắt

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

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Springer Science and Business Media LLC

Tập 17

376-392

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