Histopathological and genetic changes proved the anti-cancer potential of free and nano-capsulated sinapic acid

Applied Biological Chemistry - Tập 62 - Trang 1-10 - 2019
Doaa A. Badr1, Mohamed E. Amer2, Wagih M. Abd-Elhay3, Mohamed S. M. Nasr3, Tamer M. M. Abuamara3, Harbi Ali1, Aly F. Mohamed4, Maha A. Youssef5, Nasser S. Awwad6, Yi-Hsu Ju7,8,9, Ahmed E. Fazary1
1Applied Research Department, Research and Development Sector, Egyptian Organization for Biological Products and Vaccines (VACSERA Holding Company), Giza, Egypt
2Histology Department, Faculty of Medicine, Alazhar University, Damietta, Egypt
3Histology Department, Faculty of Medicine, Alazhar University, Cairo, Egypt
4The International Center for Advanced Researches (ICTAR- Egypt), Cairo, Egypt
5Analytical Chemistry and Control Department, Hot Laboratories Center, Atomic Energy Authority of Egypt, Cairo, Egypt
6Research Centre for Advanced Materials Science (RCAMS), King Khalid University, Abha, Saudi Arabia
7Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan
8Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan
9Taiwan Building Technology Center, National Taiwan University of Science and Technology, Taipei, Taiwan

Tóm tắt

Cancer is known to be a fierce disease that causes a large percentage of the deaths worldwide. The common cancer treatments; chemotherapy, radiotherapy and surgery are known for their severe side effects; therefore scientists are working on finding solutions to reduce these drawbacks. One of these treatment systems is the sustained released drugs formulations, these systems depend on the encapsulation of the chemotherapy within an emulsifying agent, in order to obtain a slow drug release of low doses over long time intervals. In this study, the anti-cancer effects of free and encapsulated sinapic acid was tested against lung (A549), and colon (CaCo2) cancer cell lines, along with normal fibroblast cells (HFB4) as a negative control. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed for IC50 evaluation, also cell cycle assay was performed to detect cell cycle arrest status and related anti-apoptotic and pro-apoptotic; Blc-2, BAX, and P53 gene profile fold changes post cellular treatment. Data recorded revealed that encapsulated SA showed a lower toxicity than the free form to both cell lines and also to the normal cells. The cell cycle analysis showed a cell cycle arrest at the G2/M phase post cell treatment with the free and encapsulated sinapic acid accompanied with up regulation of Bax and P53 and a down regulation of Blc-2 genes in both cell lines. The data suggest a promising anti-cancer and anti-proliferative potential of free and encapsulated sinapic acid. Also they show that the anti-cancer effect of free and encapsulated sinapic acid is quite close.

Tài liệu tham khảo

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