Histomorphologic Characterization of Noncarious and Caries‐Affected Dentin/Adhesive Interfaces

Journal of Prosthodontics - Tập 15 Số 2 - Trang 82-88 - 2006
Reem Haj‐Ali1, Mary P. Walker2, Karen Williams3, Yong Wang4, Paulette Spencer5
1Department of Restorative Dentistry, School of Dentistry, Kansas City, MO 64108, USA.
2Associate Professor, Director of Dental Biomaterials, Department of Restorative Dentistry.
3Professor, Director of Clinical Research Center, Department of Behavioral Sciences.
4Associate Professor, Department of Oral Biology
5Professor, Director of UMKC Center for Research on Interfacial Structure and Properties, Department of Oral Biology, School of Dentistry, Kansas City, MO.

Tóm tắt

Purpose : The purpose of this study was to compare the dentin/adhesive interfacial characteristics when bonding to noncarious as well as caries‐affected dentin.

Materials and Methods : Seven extracted, unerupted, third molars were sectioned into halves. Artificial caries was created on one‐half of each tooth, leaving the other half as a control. Dentin surfaces were treated with UNO adhesive according to the manufacturer's instructions for the wet‐bonding technique and under environmental conditions present in the oral cavity. Dentin/adhesive interface sections of each half‐tooth were stained with Goldner's trichrome, a classic bone stain, and examined using light microscopy. The width of exposed collagen was measured directly from photomicrographs, and adhesive penetration was analyzed qualitatively.

Results: The degree and extent to which the adhesive encapsulated the demineralized dentin matrix were reflected in the color difference in the stained sections with the noncarious dentin sections showing a degree of collagen encapsulation superior to that of the caries‐affected dentin sections. The overall mean widths of exposed collagen were significantly (p≤ .05) greater at the caries‐affected dentin/adhesive interface, 8.6 (1.7) μm, as compared with those at the noncarious dentin/adhesive interface, 6.0 (1.5) μm.

Conclusions: The morphologic characteristics of the caries‐affected dentin/interface suggest an increase in the exposed collagen zone and a decrease in the quality of the adhesive infiltration when compared with noncarious dentin. The evidence suggests that dentin substrate characteristics have a significant effect on the dentin/adhesive interface structure.

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Thông tin xuất bản

Journal of Prosthodontics

Tập 15 Số 2

82-88

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