Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Phân tích mô học, sinh lý - hóa sinh và biểu hiện gen tiết lộ các yếu tố hạn chế tiềm năng cho thành công trong việc ghép cây hồ đào
Tóm tắt
Quá trình ra chồi là một kỹ thuật ghép quan trọng để sinh sản vô tính cây hồ đào (Carya illinoinensis (Wangenh.) K. Koch). Để xác định các yếu tố có thể cản trở việc ra chồi thành công của loài này, một giống dễ sống ‘Pawnee’ và một giống khó sống điển hình ‘Jinhua’ đã được sử dụng cho phân tích toàn diện. Quan sát hình thái cho thấy rằng các tế bào xung quanh các tế bào tiết hay ống dẫn có dấu hiệu bị suy giảm ở ‘Jinhua’ nhưng không ở ‘Pawnee’ trong quá trình ghép. ‘Jinhua’ có thể gặp phải áp lực thiếu oxy nhiều hơn so với ‘Pawnee’ vì ‘Jinhua’ có hoạt tính catalase, superoxide dismutase, polyphenol oxidase, pyruvate decarboxylase (PDC), và alcohol dehydrogenase (ADH) cao hơn trong quá trình ghép và chứa mức độ hydrogen peroxide cao hơn 12 ngày sau khi ghép (DAG). Sự sao chép của PDC và ADH cũng đã được tăng cường đáng kể ở ‘Jinhua’ trong khi không bị ảnh hưởng đáng kể ở ‘Pawnee’. Hoạt tính của phenylalanine ammonia-lyase ở ‘Jinhua’ thấp hơn liên tục so với ‘Pawnee’. Hàm lượng phenol ban đầu tương tự nhau giữa hai giống. Các chất thúc đẩy ghép, bao gồm đường hòa tan, protein hòa tan và gibberellin (GA) đã không được phục hồi hoàn toàn ở ‘Jinhua’ 12 DAG trong khi hoàn toàn phục hồi ở ‘Pawnee’. Mức độ trans-zeatin riboside ở ‘Jinhua’ thấp hơn nhiều so với ở ‘Pawnee’ 3 DAG. Hàm lượng axit indole-3-acetic tương tự nhau, và động học của axit abscisic cũng giống nhau giữa hai kiểu gen. Kết quả cho thấy áp lực thiếu oxy và sự thiếu hụt đường, protein, GA, và cytokinin trong quá trình lành vết có thể là các yếu tố chủ chốt hạn chế sự thành công trong việc ra chồi của cây hồ đào. Mức độ tương thích giữa cành ghép và gốc ghép và hàm lượng phenol có thể không phải là yếu tố hạn chế cho việc ra chồi thành công.
Từ khóa
#ghép cây hồ đào #phương pháp ra chồi #áp lực thiếu oxy #hoạt động của enzyme #biểu hiện gen #sự tương thích giữa cành ghép và gốc ghép #chất thúc đẩy ghép #sinh lý cây trồngTài liệu tham khảo
Baron D, Amaro ACE, Pina A, Ferreira G (2019) An overview of grafting re-establishment in woody fruit species. Sci Horti 243:84–91. https://doi.org/10.1016/j.scienta.2018.08.012
Benjamini Y, Hochberg Y (2000) On the adaptive control of the false discovery rate in multiple testing with independent statistics. J Educ Behav Stat 25(1):60–83. https://doi.org/10.3102/10769986025001060
Bhardwaj E, Sharma D (2017) Medicinal and therapeutic properties of pecan (Carya illinoensis). Int J Herb Med 5(6):1–3
Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91(2):179–194. https://doi.org/10.1093/aob/mcf118
Cassol DA, Pirola K, Dotto M, Citadin I, Mazaro SM, Júnior AW (2016) Grafting technique and rootstock species for the propagation of Plinia cauliflora. Ciênc Rural 47(2):e20140452. https://doi.org/10.1590/0103-8478cr20160452
Emamverdian A, Ding Y, Mokhberdoran F, Xie Y, Zheng X, Wang Y (2020) Silicon dioxide nanoparticles improve plant growth by enhancing antioxidant enzyme capacity in bamboo (Pleioblastus pygmaeus) under lead toxicity. Trees 34(2):469–481. https://doi.org/10.1007/s00468-019-01929-z
Falhof J, Pedersen JT, Fuglsang AT, Palmgren M (2016) Plasma membrane H+-ATPase regulation in the center of plant physiology. Mol Plant 9(3):323–337. https://doi.org/10.1016/j.molp.2015.11.002
Gamba G, Cisse V, Donno D, Razafindrakoto ZR, Beccaro GL (2022) Quali-quantitative study on phenol compounds as early predictive markers of graft incompatibility: a case study on chestnut (Castanea spp.). Horticulturae 8(1):32. https://doi.org/10.3390/horticulturae8010032
Gautier AT, Chambaud C, Brocard L, Ollat N, Gambetta GA, Delrot S, Cookson SJ (2019) Merging genotypes: graft union formation and scion-rootstock interactions. J Exp Bot 70(3):747–755. https://doi.org/10.1093/jxb/ery422
Giri C, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees 18(2):115–135. https://doi.org/10.1007/s00468-003-0287-6
Gunes A, Pilbeam DJ, Inal A (2009) Effect of arsenic–phosphorus interaction on arsenic-induced oxidative stress in chickpea plants. Plant Soil 314(1):211–220. https://doi.org/10.1007/s11104-008-9719-9
Hayat F, Iqbal S, Coulibaly D, Razzaq MK, Nawaz MA, Jiang W, Shi T, Gao Z (2021) An insight into dwarfing mechanism: contribution of scion-rootstock interactions toward fruit crop improvement. Fruit Res 1(1):1–11
Hošek P, Hoyerová K, Kiran NS, Dobrev PI, Zahajská L, Filepová R, Motyka V, Müller K, Kamínek M (2020) Distinct metabolism of N-glucosides of isopentenyladenine and trans-zeatin determines cytokinin metabolic spectrum in Arabidopsis. New Phytol 225(6):2423–2438. https://doi.org/10.1111/nph.16310
Hunter P (2021) The molecular biology of grafting: recent research may provide new applications for a millennia-old agricultural technology. Embo Rep 22(11):e54098
Ismail AM, Ella ES, Vergara GV, Mackill DJ (2009) Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa). Ann Bot 103(2):197–209. https://doi.org/10.1093/aob/mcn211
Ismond KP, Dolferus R, De Pauw M, Dennis ES, Good AG (2003) Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway. Plant Physiol 132(3):1292–1302. https://doi.org/10.1104/pp.103.022244
Karimi H, Nowrozy M (2017) Effects of rootstock and scion on graft success and vegetative parameters of pomegranate. Sci Horti 214:280–287. https://doi.org/10.1016/j.scienta.2016.11.047
Köse C, Güleryüz M (2006) Effects of auxins and cytokinins on graft union of grapevine (Vitis vinifera). N Z J Crop Hortic Sci 34(2):145–150. https://doi.org/10.1080/01140671.2006.9514399
Li RX, He JX, Xie HG, Wang WX, Bose SK, Sun YQ, Hu JE, Yin H (2019) Effects of chitosan nanoparticles on seed germination and seedling growth of wheat (Triticum aestivum L.). Int J Biol Macromol 126:91–100. https://doi.org/10.1016/j.ijbiomac.2018.12.118
Licausi F, Kosmacz M, Weits DA, Giuntoli B, Giorgi FM, Voesenek LA, Perata P, Van Dongen JT (2011) Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization. Nature 479(7373):419–422. https://doi.org/10.1038/nature10536
López-Gómez E, San Juan M, Diaz-Vivancos P, Beneyto JM, García-Legaz M, Hernández J (2007) Effect of rootstocks grafting and boron on the antioxidant systems and salinity tolerance of loquat plants (Eriobotrya japonica Lindl.). Environ Exp Bot 60(2):151–158. https://doi.org/10.1016/j.envexpbot.2006.10.007
Lovell JT, Bentley NB, Bhattarai G, Jenkins JW, Sreedasyam A, Alarcon Y, Bock C, Boston LB, Carlson J, Cervantes K (2021) Four chromosome scale genomes and a pan-genome annotation to accelerate pecan tree breeding. Nat Commun 12(1):1–12. https://doi.org/10.1038/s41467-021-24328-w
Mayer AM (2006) Polyphenol oxidases in plants and fungi: going places? A Review Phytochemistry 67(21):2318–2331. https://doi.org/10.1016/j.phytochem.2006.08.006
Melnyk CW (2017) Plant grafting: insights into tissue regeneration. Regeneration 4(1):3–14. https://doi.org/10.1002/reg2.71
Melnyk CW, Gabel A, Hardcastle TJ, Robinson S, Miyashima S, Grosse I, Meyerowitz EM (2018) Transcriptome dynamics at Arabidopsis graft junctions reveal an intertissue recognition mechanism that activates vascular regeneration. P Nati A Sci 115(10):e2447–e2456. https://doi.org/10.1073/pnas.1718263115
Miao L, Li Q, Sun T, Chai S, Wang C, Bai L, Sun M, Li Y, Qin X, Zhang Z (2021) Sugars promote graft union development in the heterograft of cucumber onto pumpkin. Hortic Res 8(1):146. https://doi.org/10.1038/s41438-021-00580-5
Mng’omba SA, du Toit ES (2013) Effect of diagonal cut surface length on graft success and growth of Mangifera indica, Persia americana, and Prunus persica. HortScience 48(4):481–484
Mng’omba SA, du Toit ES, Akinnifesi FK (2008) The relationship between graft incompatibility and phenols in Uapaca kirkiana Müell Arg. Sci Horti 117(3):212–218. https://doi.org/10.1016/j.scienta.2008.03.031
Mo ZH, He HY, Su WC, Peng FR (2017) Analysis of differentially accumulated proteins associated with graft union formation in pecan (Carya illinoensis). Sci Horti 224:126–134. https://doi.org/10.1016/j.scienta.2017.06.005
Mo ZH, Feng G, Su WC, Liu ZZ, Peng FR (2018a) Identification of miRNAs associated with graft union development in pecan [Carya illinoinensis (Wangenh.) K. Koch]. Forests 9(8):472. https://doi.org/10.3390/f9080472
Mo ZH, Feng G, Su WC, Liu ZZ, Peng FR (2018b) Transcriptomic analysis provides insights into grafting union development in pecan (Carya illinoinensis). Genes 9(2):71. https://doi.org/10.3390/genes9020071
Mo ZH, Chen YQ, Lou WR, Jia XD, Zhai M, Xuan JP, Guo ZR, Li YR (2020) Identification of suitable reference genes for normalization of real-time quantitative PCR data in pecan (Carya illinoinensis). Trees 34:1233–1241. https://doi.org/10.1007/s00468-020-01993-w
Nanda AK, Melnyk CW (2018) The role of plant hormones during grafting. J Plant Res 131(1):49–58. https://doi.org/10.1007/s10265-017-0994-5
Nesbitt ML, Goff WD, Stein LA (2002) Effect of scionwood packing moisture and cut-end sealing on pecan graft success. HortTechnology 12(2):257–260
Pereira IDS, Messias RDS, Campos ÂD, Errea P, Antunes LC, Fachinello J, Pina A (2014) Growth characteristics and phenylalanine ammonia-lyase activity in peach grafted on different Prunus spp. Biol Plantarum 58(1):114–120. https://doi.org/10.1007/s10535-013-0370-9
Rezaee R, Vahdati K, Grigoorian V, Valizadeh M (2008) Walnut grafting success and bleeding rate as affected by different grafting methods and seedling vigour. J Hortic Sci Biotech 83(1):94–99. https://doi.org/10.1080/14620316.2008.11512352
Ribeiro LM, Nery LA, Vieira LM, Mercadante-Simões MO (2015) Histological study of micrografting in passionfruit. Plant Cell Tiss Org 123(1):173–181. https://doi.org/10.1007/s11240-015-0824-1
Roberts JK, Chang K, Webster C, Callis J, Walbot V (1989) Dependence of ethanolic fermentation, cytoplasmic pH regulation, and viability on the activity of alcohol dehydrogenase in hypoxic maize root tips. Plant Physiol 89(4):1275–1278. https://doi.org/10.1104/pp.89.4.1275
Usenik V, Krška B, Vičan M, Štampar F (2006) Early detection of graft incompatibility in apricot (Prunus armeniaca L.) using phenol analyses. Sci Horti 109(4):332–338. https://doi.org/10.1016/j.scienta.2006.06.011
Wang XW, Chatwin W, Hilton A, Kubenka K (2022) Genetic diversity revealed by microsatellites in genus Carya. Forests 13(2):188. https://doi.org/10.3390/f13020188
Warmund MR, Cumbie BG, Coggeshall MV (2012) Stem anatomy and grafting success of Chinese chestnut scions on ‘AU-Cropper’ and ‘Qing’ seedling rootstocks. HortScience 47(7):893–895
Williams LE, Lemoine R, Sauer N (2000) Sugar transporters in higher plants–a diversity of roles and complex regulation. Trends Plant Sci 5(7):283–290. https://doi.org/10.1016/S1360-1385(00)01681-2
Yang ZJ, Feng JL, Chen H (2013) Study on the anatomical structures in development of the nurse seed grafted union of Camellia oleifera. Plant Sci J 31(3):313–320. https://doi.org/10.3724/SP.J.1142.2013.30313
Yin H, Yan B, Sun J, Jia PF, Zhang ZJ, Yan XS, Chai J, Ren ZZ, Zheng GC, Liu H (2012) Graft-union development: a delicate process that involves cell–cell communication between scion and stock for local auxin accumulation. J Exp Bot 63(11):4219–4232. https://doi.org/10.1093/jxb/ers109
Yoshida T, Christmann A, Yamaguchi-Shinozaki K, Grill E, Fernie AR (2019) Revisiting the basal role of ABA–roles outside of stress. Trends Plant Sci 24(7):625–635. https://doi.org/10.1016/j.tplants.2019.04.008
Zhai LM, Wang XM, Tang D, Qi Q, Yer H, Jiang XN, Han ZH, McAvoy R, Li W, Li Y (2021) Molecular and physiological characterization of the effects of auxin-enriched rootstock on grafting. Hortic Res 8(1):74. https://doi.org/10.1038/s41438-021-00509-y
Zhang X, Liu CJ (2015) Multifaceted regulations of gateway enzyme phenylalanine ammonia-lyase in the biosynthesis of phenylpropanoids. Mol Plant 8(1):17–27. https://doi.org/10.1016/j.molp.2014.11.001
Zhang C, Liu F, Kong WW, He Y (2015a) Application of visible and near-infrared hyperspectral imaging to determine soluble protein content in oilseed rape leaves. Sensors 15(7):16576–16588. https://doi.org/10.3390/s150716576
Zhang R, Peng FR, Li YR (2015b) Pecan production in China. Sci Horti 197:719–727. https://doi.org/10.1016/j.scienta.2015.10.035
Zhang R, Peng FR, Le DL, Liu ZZ, He HY, Liang YW, Tan PP, Hao MZ, Li YR (2015c) Evaluation of epicotyl grafting on 25-to 55-day-old pecan seedlings. HortTechnology 25(3):392–396
Zhang HL, Deng C, Wu X, Yao J, Zhang YL, Zhang YN, Deng SR, Zhao N, Zhao R, Zhou XY, Lu CF, Lin SZ, Chen SL (2020) Populus euphratica remorin 65 activates plasma membrane H+-ATPases to mediate salt tolerance. Tree Physiol 40(6):731–745. https://doi.org/10.1093/treephys/tpaa022
Zhang MX, Wang Y, Chen X, Xu FY, Ding M, Ye WX, Kawai Y, Toda Y, Hayashi Y, Suzuki T, Zeng HQ, Xiao L, Xiao X, Xu J, Guo SW, Yan F, Shen QR, Xu GH, Kinoshita T, Zhu YY (2021) Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis. Nat Commun 12(1):1–12. https://doi.org/10.1038/s41467-021-20964-4
Zhang CC, Ren HD, Yao XH, Wang KL, Chang J (2022) Comparative transcriptome analysis reveals differential regulation of flavonoids biosynthesis between kernels of two pecan cultivars. Front Plant Sci 13:804968. https://doi.org/10.3389/fpls.2022.804968
Zheng BS, Chu HL, Jin SH, Huang YJ, Wang ZJ, Chen M, Huang JQ (2010) cDNA-AFLP analysis of gene expression in hickory (Carya cathayensis) during graft process. Tree Physiol 30(2):297–303. https://doi.org/10.1093/treephys/tpp102
Zhou YC, Underhill SJ (2018) Plasma membrane H+-ATP ase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus). Plant Biol 20(6):978–985. https://doi.org/10.1111/plb.12879
Zhou Q, Gao B, Li WF, Mao J, Yang SJ, Li W, Ma ZH, Zhao X, Chen BH (2020) Effects of exogenous growth regulators and bud picking on grafting of grapevine hard branches. Sci Horti 264:109186. https://doi.org/10.1016/j.scienta.2020.109186