Highly efficient expression and purification system of small-size protein domains in Escherichia coli for biochemical characterization

Guan, 1991, Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase, Anal. Biochem., 192, 262, 10.1016/0003-2697(91)90534-Z

Guan, 1988, Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein, Gene, 67, 21, 10.1016/0378-1119(88)90004-2

LaVallie, 1993, A thioredoxin gene fusion expression system that circumvents inclusion-body formation in the E. coli cytoplasm, Biotechnology, 11, 187, 10.1038/nbt0293-187

Tenno, 2004, High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling, Protein Eng. Des. Sel., 174, 305, 10.1093/protein/gzh044

Lindhout, 2003, High-yield expression of isotopically labeled peptides for use in NMR studies, Protein Sci., 12, 1786, 10.1110/ps.0376003

Jones, 2000, Expression and membrane assembly of a transmembrane region from Neu, Biochemistry, 39, 1870, 10.1021/bi992495e

Sharon, 2002, Expression, purification, and isotope labeling of a gp120 V3 peptide and production of a Fab from a HIV-1 neutralizing antibody for NMR studies, Prot. Exp. Purif., 24, 374, 10.1006/prep.2001.1577

Gronenborn, 1991, A novel, highly stable fold of the immunoglobulin binding domain of streptococcal protein G, Science, 253, 657, 10.1126/science.1871600

Cheng, 2004, An efficient system for small protein expression and refolding, Biochem. Biophys. Res. Commun., 317, 401, 10.1016/j.bbrc.2004.03.068

Burns, 2000, Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor, Nat. Cell Biol., 2, 346, 10.1038/35014038

Faber, 1998, Huntingtin interacts with a family of WW domain proteins, Hum. Mol. Genet., 7, 1463, 10.1093/hmg/7.9.1463

Hofmann, 2001, A ubiquitin-interacting motif conserved in components of the proteasomal and lysosomal protein degradation systems, Trends Biochem. Sci., 26, 347, 10.1016/S0968-0004(01)01835-7

Shimada, 1991, Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA, Science, 254, 576, 10.1126/science.1948034

Miake, 2002, Biochemical characterization of the core structure of alpha-synuclein filaments, J. Biol. Chem., 277, 19213, 10.1074/jbc.M110551200

Higucchi, 1988, A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions, Nucleic Acids Res., 16, 7351, 10.1093/nar/16.15.7351

Zhou, 2001, A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins, J. Biomol. NMR, 20, 11, 10.1023/A:1011258906244