Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells

Biogerontology - Tập 12 - Trang 565-579 - 2011
Seong-Hoon Park1,2, Hong-Jun Kang3, Hyun-Seok Kim1,2, Min-Ju Kim4, Jee-In Heo5,6, Jeong-Hyeon Kim5, Yoon-Jung Kho6, Sung Chan Kim5, Jaebong Kim5, Jae-Bong Park5, Jae-Yong Lee5,6
1Department of Radiation Oncology, Vanderbilt University, Nashville, USA
2Department of Pediatrics, Vanderbilt University, Nashville, USA
3Genetic Disease Research Section, NIDDK, National Institutes of Health, Bethesda, USA
4Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, Korea (South)
5Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Korea (South)
6Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon, Korea (South)

Tóm tắt

Since the detailed comparison of DNA repair activities among mammalian embryonic fibroblast cells with different replicative life spans has not been investigated, we tested DNA repair activities in embryonic fibroblast cells derived from mammals including human, dog, rat, and mouse. The cell viability after treatment of four DNA damage agents appeared to be decreased in the order of human embryonic fibroblasts (HEFs) > dog embryonic fibroblasts (DEFs) > rat embryonic fibroblasts (REFs) > mouse embryonic fibroblasts (MEFs) although statistical significance was lacking. The amounts of strand breaks and AP (apurinic/apyrimidinic) sites also appear to be decreased in the order of HEFs > DEFs > REFs ≥ MEFs after treatment of DNA damage agents. The DNA repair activities and rates including base excision repair (BER), nucleotide excision repair (NER) and double-strand break repair (DSBR) including non-homologous end-joining (NHEJ) decreased again in the order of HEFs > DEFs > REFs ≥ MEFs. BER and NHEJ activities in 3% O2 also decreased in the order of HEFs > DEFs > REFs > MEFs. This order in DNA repair activity appears to be coincident with that of replicative life span of fibroblasts and that of life span of mammals. These results indicate that higher DNA repair activity is related with longer replicative life span in embryonic fibroblast cells.

Tài liệu tham khảo

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