High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors

Oxford University Press (OUP) - Tập 37 - Trang 431-435 - 2010
Anita Rywińska1, Waldemar Rymowicz1
1Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

Tóm tắt

An acetate negative mutant of Yarrowia lipolytica Wratislavia AWG7 was found to be suitable for the production of high amounts of citric acid in long-term repeated-batch cultures. When 40% of fresh replaced medium was fed, this strain produced 154 g l−1, on average, which corresponded to a 0.78 g g−1 yield and a productivity of 1.05 g l−1 h−1. The activity of the culture remained stable for more than 1,650 h, i.e., 16 cycles of the repeated-batch bioreactors.

Tài liệu tham khảo

Anastassiadis S, Morgunov IG, Kamzolova SV, Finogenova TV (2008) Citric acid production patent review. Recent Pat Biotechnol 2(2):1–17 Anastassiadis S, Rhem HJ (2006) Citric acid production from glucose by yeast Candida oleophila ATCC 20177 under batch, continuous and repeated batch cultivation. Electron J Biotechnol 9:26–39 Arzumanov TE, Shishkanova NV, Finogenova TV (2000) Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol. Appl Microbiol Biotechnol 53:525–529 Crolla A, Kennedy KJ (2001) Optimization of citric acid production from Candida lipolytica Y-1095 using n-paraffin. J Biotechnol 89:27–40 Finogenova TV, Morgunov IG, Kamzolova SV, Cheryavskaya OG (2005) Organic acid production by the yeast Yarrowia lipolytica: a review of prospects. Appl Biochem Microbiol 41:418–425 Förster A, Aurich A, Mauersberger S, Barth G (2007) Citric acid production from sucrose using a recombinant strain of the yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 75:1409–1417. doi:10.1007/s00253-002-1022-8 Goldberg D, Ellis G (1983) Isocitrate dehydrogenase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, Weinheim Imandi SB, Bandaru VVR, Somalanka SR, Garapati HR (2007) Optimization of medium constituents for the production of citric acid from byproduct glycerol using Doehlert experimental design. Enzyme Microb Technol 40:1367–1372. doi:10.1016/j.enzmictec.2006.10.012 Kamzolova SV, Morgunov IG, Aurich A, Perevoznikova OA, Shishkanova NV, Stottmeister U, Finogenova TV (2005) Lipase secretion and citric acid production in Yarrowia lipolytica yeast grown on animal and vegetable fat. Food Technol Biotechnol 43:113–122 Kautola H (1990) Itaconic acid production from xylose in repeated-batch and continuous bioreactors. Appl Microbiol Biotechnol 33(1):7–11 Levinson WE, Kurtzman CP, Kuo TM (2007) Characterization of Yarrowia lipolytica and related species for citric acid production from glycerol. Enzyme Microb Technol 41:292–295. doi:10.1016/j.enzmictec.2007.02.005 Moresi M (1994) Effect of glucose concentration on citric acid production by Yarrowia lipolytica. J Chem Technol Biotechnol 60(4):387–395 Papanikolaou S, Aggelis G (2003) Modelling aspects of the biotechnological valorization of crude glycerol: production of citric acid by Yarrowia lipolytica and 1,3-propanediol by Clostridium butyricum. J Chem Technol Biotechnol 78:542–547. doi:10.1002/jctb.831 Papanikolaou S, Fakas S, Fick M, Chevalot I, Galiotou-Panayotou M, Komaitis M, Marc I, Aggelis G (2008) Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil. Biomass Bioenerg 32:60–71. doi:10.1016/j.biombioe.2007.06.007 Papanikolaou S, Muniglia L, Chevalot I, Aggelis A, Marc I (2002) Yarrowia lipolytica as a potential producer of citric acid from raw glycerol. J Appl Microbiol 92:737–744 Rymowicz W, Rywińska A, Żarowska B, Juszczyk P (2006) Citric acid production from crude glycerol by acetate mutants of Yarrowia lipolytica. Chem Pap 60:391–394. doi:10.2478/s11696-006-0071-3 Rywińska A, Rymowicz W, Żarowska B, Wojtatowicz M (2009) Biosynthesis of citric acid from glycerol by acetate mutants of Yarrowia lipolytica in fed-batch fermentation. Food Technol Biotechnol 47(1):1–6 Sakurai A, Nishida Y, Saito H, Sakakibara M (2000) Ethanol production by repeated batch culture using yeast cells immobilized within porous cellulose carriers. J Biosci Bioeng 90(5):526–529. doi:10.1016/S1389-1723(01)80034-7 Stewart PR (1975) Analytical methods for yeasts. In: Prescot DM (ed) Methods in cell biology. Academic Press, New York Soccol CR, Vandenberghe LPS, Rodrigues C, Pandey A (2006) New perspectives for citric acid production and application. Food Technol Biotechnol 44:141–149 Yu MC, Wang RC, Wang CY, Duan KJ, Sheu DC (2007) Enhanced production of l(+)-lactic acid by floc-form culture of Rhizopus oryzae. J Chin Inst Chem Eng 38(3–4):223–228