High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides

Journal of Clinical Pathology - Tập 57 Số 6 - Trang 644-646 - 2004
Beatriz Carvalho1, E Ouwerkerk2, Gerrit A. Meijer1, Bauke Ylstra3
1Department of Pathology, VU University Medical Centre, De Boelelaan 1117, 1081HV Amsterdam, The Netherlands
2Microarray Core Facility, Medical Faculty, VU University Medical Centre, van der Boechorststraat 7-9, 1081BT Amsterdam, The Netherlands
3Medical Faculty, VU University Medical Centre, van der Boechorststraat 7-9, 1081BT Amsterdam, The Netherlands

Tóm tắt

Background:Currently, comparative genomic hybridisation array (array CGH) is the method of choice for studying genome wide DNA copy number changes. To date, either amplified representations of bacterial artificial chromosomes (BACs)/phage artificial chromosomes (PACs) or cDNAs have been spotted as probes. The production of BAC/PAC and cDNA arrays is time consuming and expensive.Aim:To evaluate the use of spotted 60 mer oligonucleotides (oligos) for array CGH.Methods:The hybridisation of tumour cell lines with known chromosomal aberrations on to either BAC or oligoarrrays that are mapped to the human genome.Results:Oligo CGH was able to detect amplifications with high accuracy and greater spatial resolution than other currently used array CGH platforms. In addition, single copy number changes could be detected with a resolution comparable to conventional CGH.Conclusions:Oligos are easy to handle and flexible, because they can be designed for any part of the genome without the need for laborious amplification procedures. The full genome array, containing around 30 000 oligos of all genes in the human genome, will represent a big step forward in the analysis of chromosomal copy number changes. Finally, oligoarray CGH can easily be used for any organism with a fully sequenced genome.

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Tài liệu tham khảo

10.1038/75985

10.1038/ng771

10.1073/pnas.162471999

2002, Cancer Res, 62, 957

10.1038/sj.onc.1206621

10.1038/sj.onc.1206350

10.1038/ng754

1999, Nat Genet, 23, 41

2002, Cancer Res, 62, 6240

10.1126/science.2999974

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Journal of Clinical Pathology

Tập 57 Số 6

644-646

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