High-performance thin-layer chromatography fingerprint profile analysis and spectro-densitometric evaluation of antiproliferative antioxidants such as ellagic acid and gallic acid from four widely used Terminalia species

Akademiai Kiado Zrt. - Tập 36 - Trang 169-178 - 2023
Chaitrali M. Bidikar1,2, Pramod J. Hurkadale1, Shrikrishna M. Nandanwadkar3, Harsha V. Hegde4, Sneha Singh5, Abhijeet Khale5, Manjusha Phanse5
1Department of Pharmacognosy and Phytochemistry, KLE College of Pharmacy, KLE Academy of Higher Education and Research (KAHER), (Deemed-to-Be University), Belagavi, India
2Dr. Prabhakar Kore Basic Science Research Center, KLE Academy of Higher Education and Research, (Deemed-to-Be University), Belagavi, India
3A7-A8 Hitachi-Enviro House Center of Excellence, Mumbai, India
4ICMR-National Institute of Traditional Medicine, Belagavi, India
5Anchrom Enterprises (I) Pvt Ltd, Mulund, Mumbai, India

Tóm tắt

The emerging need for quality control to assess the chemical composition and stability of herbal raw materials has grown significantly along with their demand. The proposed research focuses on phytochemical analysis of hydro-alcoholic bark and fruit extracts from Terminalia (arjuna, bellirica, chebula, and catappa) followed by high-performance thin-layer chromatography (HPTLC) technique. HPTLC fingerprinting carried out on precoated silica gel F254 TLC revealed the presence of alkaloids, flavonoids, tannins, phenols, and antioxidants. The developed RP-HPTLC method was validated to quantify gallic acid (GA) and ellagic acid (EA). The calibration plot from linear regression analysis demonstrated a good polynomial regression relationship, with R values of 99.99% for the peak areas of GA and EA, respectively. The calibration range for GA and EA is 100–700 ng per band. Spectro-densitometric scanning verified GA and EA by four-way confirmation via RF, chromatogram, spectra, and visual comparison of chromatograms. The method was developed and validated in accordance with the International Council for Harmonisation guidelines. From the validation results, it can be concluded that this method would prove an excellent alternative to existing costly techniques such as gas chromatography and high-performance liquid chromatography.

Tài liệu tham khảo

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