High performance liquid chromatography assay for determination of moxifloxacin in human plasma

Vietnam Journal of Science and Technology - Tập 57 Số 3 - Trang 336-343 - 2019
Luong Thi My Hanh1, Nguyen Thi Minh Diep2, Pham Thi Ngoc Mai1, Nguyen Xuan Truong3
1Faculty of Chemistry, Hanoi University of Science, VNU Ha Noi, 19 Le Thanh Tong street, Hoan Kiem, Ha Noi, Viet Nam
2Department of Drug Quality Control, Phu Tho Medical and Pharmacy College, No. 2201, Hung Vuong street, Viet Tri, Phu Tho, Viet Nam
3School of Chemical Engineering, Hanoi University of Science and Technology, No 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam

Tóm tắt

A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with tricloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25cm×4.5mm, 5µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (70:30, v/v) as mobile phase at a flow rate of 0.8 ml/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30%. The calibration curve was linear over the concentration range of 0.3 to 25.0 µg/mL with coefficient correlation of 0.9991. This method was successfully applied for therapeutic drug monitoring.

Từ khóa

#Moxifloxacin #protein precipitation #tricloroacetic acid #HPLC method #human plasma

Tài liệu tham khảo

(1) R. Ronald, A.; Low, D. Fluoroquinolone Antibiotics.; Birkhauser Boston Springer: Cambridge; Secaucus, 2012.

(2) The Quinolones, 3rd ed.; Andriole, V. T., Ed.; Academic Press: San Diego, 2000.

(3) Appelbaum, P. C.; Hunter, P. A. The Fluoroquinolone Antibacterials: Past, Present and Future Perspectives. Int. J. Antimicrob. Agents 2000, 16 (1), 5–15.

(4) Labandjurdjevic, A.; Jelikicstankov, M.; Djurdjevic, P. Optimization and Validation of the Direct HPLC Method for the Determination of Moxifloxacin in Plasma. J. Chromatogr. B 2006, 844 (1), 104–111. https://doi.org/10.1016/j.jchromb.2006.07.001.

(5) Tatar Ulu, S. High-Performance Liquid Chromatography Assay for Moxifloxacin: Pharmacokinetics in Human Plasma. J. Pharm. Biomed. Anal. 2007, 43 (1), 320–324. https://doi.org/10.1016/j.jpba.2006.06.026.

(6) Hemanth Kumar, A. K.; Ramachandran, G. Simple and Rapid Liquid Chromatography Method for Determination of Moxifloxacin in Plasma. J. Chromatogr. B 2009, 877 (11–12), 1205–1208. https://doi.org/10.1016/j.jchromb.2009.02.042.

(7) Hurtado, F. K.; Kaiser, M.; Tasso, L.; Dalla Costa, T. Fast and Sensitive RP-HPLC—Fluorescence Method for the Quantitative Analysis of Moxifloxacin in Rat Plasma and Its Application to a Preclinical Pharmacokinetic Study. Acta Chromatogr. 2016, 28 (2), 175–191. https://doi.org/10.1556/1326.2016.28.2.3.

(8) Khan, F. U.; Nasir, F.; Iqbal, Z.; Khan, I.; Shahbaz, N.; Hassan, M.; Ullah, F. Simultaneous Determination of Moxifloxacin and Ofloxacin in Physiological Fluids Using High Performance Liquid Chromatography with Ultraviolet Detection. J. Chromatogr. B 2016, 1017–1018, 120–128. https://doi.org/10.1016/j.jchromb.2016.03.002.

(9) Czyrski, A.; Sokół, A.; Szałek, E. HPLC Method for Determination of Moxifloxacin in Plasma and Its Application in Pharmacokinetic Analysis. J. Liq. Chromatogr. Relat. Technol. 2017, 40 (1), 8–12. https://doi.org/10.1080/10826076.2017.1280680.

(10) Wang, N.; Zhu, L.; Zhao, X.; Yang, W.; Sun, H. Improved HPLC Method for the Determination of Moxifloxacin in Application to a Pharmacokinetics Study in Patients with Infectious Diseases. ISRN Pharmacol. 2013, 2013, 1–7. https://doi.org/10.1155/2013/462918.

(11) Xu, Y. H.; Li, D.; Liu, X. Y.; Li, Y. Z.; Lu, J. High Performance Liquid Chromatography Assay with Ultraviolet Detection for Moxifloxacin: Validation and Application to a Pharmacokinetic Study in Chinese Volunteers. J. Chromatogr. B 2010, 878 (32), 3437–3441. https://doi.org/10.1016/j.jchromb.2010.10.024.

(12) Trần Cao Sơn. Thẩm Định Phương Pháp Trong Phân Tích Hóa Học & vi Sinh Vật; Nhà xuất bản Khoa học và Kỹ thuật, 2010.

Thông tin
Thông tin xuất bản

Vietnam Journal of Science and Technology

Tập 57 Số 3

336-343

Thông tin tác giả