Cảm Biến Phát Quang Đặc Hiệu Cao Để Phát Hiện SO32− và Hình Ảnh Sinh Học

Journal of Fluorescence - Tập 31 - Trang 363-371 - 2021
Jianbin Chao1, Jiamin Zhao1,2, Yongbin Zhang3, Fangjun Huo3, Caixia Yin4, Ming Li1,2, Yuexiang Duan1,2
1Scientific Instrument Center, Shanxi University, Taiyuan, People’s Republic of China
2School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, People’s Republic of China
3Research Institute of Applied Chemistry, Shanxi University, Taiyuan, People’s Republic of China
4Institute of Molecular Science, Shanxi University, Taiyuan, People’s Republic of China

Tóm tắt

Sulfit (SO32−) được biết đến với vai trò không thể thiếu trong nhiều quá trình sinh lý. Mức độ anormal của SO32− có thể kích hoạt nhiều loại bệnh liên quan đến hệ hô hấp, hệ thần kinh và hệ tim mạch. Do đó, việc tìm kiếm một phương pháp phát hiện SO32− hiệu quả là hết sức cần thiết. Trong nghiên cứu này, một dẫn xuất pyren, (E)-4-(3-oxo-3-(pyren-1-yl)prop-1-en-1-yl)phenyl acrylate (PPA), đã được thiết kế và tổng hợp nhằm giám sát SO32−. Cảm biến này có các bước tổng hợp đơn giản, khả năng kháng nhiễu tuyệt vời và phản ứng đặc hiệu với SO32− trong sự hiện diện của các chất khác. Phản ứng giữa PPA và SO32− được quy cho phản ứng cộng Michael và cơ chế phát hiện được xác nhận qua phân tích quang phổ HRMS và FTIR. Hơn nữa, PPA phản ứng tuyến tính để phát hiện SO32− trong khoảng 0–100 μM. Giới hạn phát hiện được tính toán là thấp tới 0,17 μM theo khuyến nghị của IUPAC (CDL = 3sb/m). Đặc biệt, PPA còn được ứng dụng trong hình ảnh sinh học tế bào HepG2, mở ra khả năng theo dõi SO32− trong cơ thể sống.

Từ khóa

#sulfite #phát hiện chất #phát quang #cơ chế phát hiện #hình ảnh sinh học #pyrene

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