High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva

Angewandte Chemie - International Edition - Tập 60 Số 45 - Trang 24266-24274 - 2021
Zijie Zhang1,2, Richa Pandey3,2, Jiuxing Li1,2, Jimmy Gu1, Dawn White4, Hannah D. Stacey1,5,6, Jann C. Ang1,5,6, Catherine‐Jean Steinberg7, Alfredo Capretta4,6, Carlos D. M. Filipe8, Karen L. Mossman7,6, Cynthia Balion9, Matthew S. Miller1,5,6, Bruno J. Salena7, Deborah Yamamura9,6, Leyla Soleymani3,10, John D. Brennan4, Yingfu Li4,1,6,10
1Department of Biochemistry and Biomedical Sciences, McMaster University, Canada
2these authors contributed equally to this work
3Department of Engineering Physics, McMaster University, Canada
4Biointerfaces Institute, McMaster University, Canada
5McMaster Immunology Research Centre, McMaster University, Canada
6Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, Canada
7Department of Medicine, McMaster University, Canada
8Department of Chemical Engineering, McMaster University, Canada
9Department of Pathology and Molecular Medicine, McMaster University, Canada
10School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1 Canada

Tóm tắt

Abstract

We report a simple and rapid saliva‐based SARS‐CoV‐2 antigen test that utilizes a newly developed dimeric DNA aptamer, denoted as DSA1N5, that specifically recognizes the spike proteins of the wildtype virus and its Alpha and Delta variants with dissociation constants of 120, 290 and 480 pM, respectively, and binds pseudotyped lentiviruses expressing the wildtype and alpha trimeric spike proteins with affinity constants of 2.1 pM and 2.3 pM, respectively. To develop a highly sensitive test, DSA1N5 was immobilized onto gold electrodes to produce an electrochemical impedance sensor, which was capable of detecting 1000 viral particles per mL in 1:1 diluted saliva in under 10 min without any further sample processing. Evaluation of 36 positive and 37 negative patient saliva samples produced a clinical sensitivity of 80.5 % and specificity of 100 % and the sensor could detect the wildtype virus as well as the Alpha and Delta variants in the patient samples, which is the first reported rapid test that can detect any emerging variant of SARS‐CoV‐2.

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Angewandte Chemie - International Edition

Tập 60 Số 45

24266-24274

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