Hetero-diploid pollen grains that represent self-compatibility are incompatible with non-self receptors in tetraploid Chinese cherry (Prunus pseudocerasus Lindl)
Tóm tắt
Chinese cherry (Prunus pseudocerasus) has many natural tetraploid species within Prunus. The pollen grains of tetraploid Chinese cherry are hetero-diploid, the two S-haplotypes in the pollen are a combination of two of the four possible S-haplotypes. The abnormal segregation ratios of pollen-S indicate that a few hetero-diploid pollen grains could inactivate self-stylar S-RNase inside the pollen tube and grow better into the self-ovaries than to the others. In this study, three Chinese cherry cultivars, “Daiba” (S
1
S
2
S
5
S
8
), “Taishanganying” (S
1
S
2
S
4
S
6
), and “Laiyangduanzhi” (S
1
S
2
S
8
S
x
), were used to investigate the inheritance of hetero-diploid pollen-S alleles in non-self receptors. Genetic analysis showed that the distribution of S-haplotypes is unequally expressed in self- and cross-pollinated progenies. The S
2
-haplotype, which is found with lowest frequency in all progeny plants, is a likely lethal mutation. Moreover, the number of individuals with two different S-haplotypes was also unequal in the two cross-pollinated progenies. Notably, the number of individuals with S
1
S
5
and S
1
S
8
genotypes was larger than other genotypes in the cross-pollinated progeny of “Laiyangduanzhi” × “Daiba”, and the number of individuals with S
1
S
4
, S
1
S
6
, and S
4
S
6
genotypes was larger than S
2
-haplotypes in the cross-pollinated progeny of “Laiyangduanzhi” × “Taishanganying”. These results indicate that only a few genotypes of hetero-diploid pollen grains have the capability to grow into the ovaries of “Laiyangduanzhi”. Interestingly, the pollen grains with S
5
S
8
genotypes, which is self-compatible, was incompatible with the styles of “Laiyangduanzhi”, while the pollen grains with S
1
S
8
, S
1
S
6
and S
4
S
6
genotypes, which are self-incompatible, were compatible with the styles of “Laiyangduanzhi”.
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