Herpes simplex virus-binding IgG traps HSV in human cervicovaginal mucus across the menstrual cycle and diverse vaginal microbial composition

Gipson, 2001, Mucins of the human endocervix, Front. Biosci., 6, D1245, 10.2741/Gipson Kieweg, 2004, Gravity‐induced coating flows of vaginal gel formulations: In vitro experimental analysis, Journal of Pharmaceutical Sciences, 93, 2941, 10.1002/jps.20194 Nguyen, 2014, Innate and adaptive immune responses in male and female reproductive tracts in homeostasis and following HIV infection, Cell. Mol. Immunol., 11, 410, 10.1038/cmi.2014.41 Cone, 2009, Barrier properties of mucus, Adv. Drug Deliv. Rev., 61, 75, 10.1016/j.addr.2008.09.008 Cole, 2006, Innate host defense of human vaginal and cervical mucosae, Curr. Top. Microbiol Immunol., 306, 199 Naz, 2012, Female genital tract immunity: distinct immunological challenges for vaccine development, J. Reprod. Immunol., 93, 1, 10.1016/j.jri.2011.09.005 Kumamoto, 2012, Unique features of antiviral immune system of the vaginal mucosa, Curr. Opin. Immunol., 24, 411, 10.1016/j.coi.2012.05.006 Chen, 2014, Transient antibody-mucin interactions produce a dynamic molecular shield against viral invasion, Biophys. J., 106, 2028, 10.1016/j.bpj.2014.02.038 Wessler, 2015, Using Computational Modeling To Optimize the Design of Antibodies That Trap Viruses in Mucus, ACS Infectious Diseases, 2, 82, 10.1021/acsinfecdis.5b00108 Fahrbach, 2013, Differential binding of IgG and IgA to mucus of the female reproductive tract, PLoS One, 8, e76176, 10.1371/journal.pone.0076176 Wang, 2016, Diffusion of immunoglobulin G in shed vaginal epithelial cells and in cell-free regions of human cervicovaginal mucus, PLoS One, 11, e0158338, 10.1371/journal.pone.0158338 Saltzman, 1994, Antibody diffusion in human cervical mucus, Biophys. J., 66, 508, 10.1016/S0006-3495(94)80802-1 Olmsted, 2001, Diffusion of macromolecules and virus-like particles in human cervical mucus, Biophys. J., 81, 1930, 10.1016/S0006-3495(01)75844-4 Wang, 2014, IgG in cervicovaginal mucus traps HSV and prevents vaginal herpes infections, Mucosal Immunol., 7, 1036, 10.1038/mi.2013.120 Sharif K. The Structure, Chemistry and Physics of Human Cervical Mucus, Vol. 2nd edn. (Blackwell Publishing Ltd, Oxford, 2006). Moncla, 2016, The Effects of Hormones and Vaginal Microflora on the Glycome of the Female Genital Tract: Cervical-Vaginal Fluid, PLoS One, 11, e0158687, 10.1371/journal.pone.0158687 Chappell, 2014, The effects of reproductive hormones on the physical properties of cervicovaginal fluid, Am. J. Obstet. Gynecol., 211, 226 e221, 10.1016/j.ajog.2014.03.041 Aksoy, 2012, The effect of ethinyl estradiol and drospirenone-containing oral contraceptives upon mucoprotein content of cervical mucus, Eur. J. Obstet. Gynecol. Reprod. Biol., 164, 40, 10.1016/j.ejogrb.2012.05.002 Shukair, 2013, Human cervicovaginal mucus contains an activity that hinders HIV-1 movement, Mucosal Immunol., 6, 427, 10.1038/mi.2012.87 Moncla, 2015, Impact of bacterial vaginosis, as assessed by nugent criteria and hormonal status on glycosidases and lectin binding in cervicovaginal lavage samples, PLoS One, 10, e0127091, 10.1371/journal.pone.0127091 Nunn, 2015, Enhanced Trapping of HIV-1 by Human Cervicovaginal Mucus Is Associated with Lactobacillus crispatus-Dominant Microbiota, MBio, 6, e01084, 10.1128/mBio.01084-15 Gajer, 2012, Temporal dynamics of the human vaginal microbiota, Sci. Transl. Med, 4, 132ra152, 10.1126/scitranslmed.3003605 Ravel, 2011, Vaginal microbiome of reproductive-age women, Proc. Natl Acad. Sci. USA, 108, 4680, 10.1073/pnas.1002611107 Ma, 2012, Vaginal microbiome: rethinking health and disease, Annu Rev. Microbiol, 66, 371, 10.1146/annurev-micro-092611-150157 Borgdorff, 2016, Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier, Mucosal Immunol., 9, 621, 10.1038/mi.2015.86 Lai, 2009, Human Immunodeficiency Virus Type 1 Is Trapped by Acidic but Not by Neutralized Human Cervicovaginal Mucus, J. Virol., 83, 11196, 10.1128/JVI.01899-08 Wira, 2014, Regulation of mucosal immunity in the female reproductive tract: the role of sex hormones in immune protection against sexually transmitted pathogens, Am. J. Reprod. Immunol., 72, 236, 10.1111/aji.12252 Lai, 2010, Nanoparticles reveal that human cervicovaginal mucus is riddled with pores larger than viruses, Proc. Natl Acad. Sci. USA, 107, 598, 10.1073/pnas.0911748107 Whaley, 2013, Antibody-based concepts for multipurpose prevention technologies, Antivir. Res, 100, S48, 10.1016/j.antiviral.2013.09.027 Zeitlin, 2011, Enhanced potency of a fucose-free monoclonal antibody being developed as an Ebola virus immunoprotectant, Proc. Natl Acad. Sci. USA, 108, 20690, 10.1073/pnas.1108360108 Klipping, 2008, Suppression of ovarian activity with a drospirenone-containing oral contraceptive in a 24/4 regimen, Contraception, 78, 16, 10.1016/j.contraception.2008.02.019 Han, 2017, Cervical mucus and contraception: what we know and what we don't, Contraception, 96, 310, 10.1016/j.contraception.2017.07.168 Clementi, 2017, Role and potential therapeutic use of antibodies against herpetic infections, Clin. Microbiol Infect., 23, 381, 10.1016/j.cmi.2016.12.023 Yamamoto, 2016, Patterns of HIV/SIV Prevention and Control by Passive Antibody Immunization, Front Microbiol, 7, 1739, 10.3389/fmicb.2016.01739 Whaley, 2014, Strategies for preventing mucosal cell-associated HIV transmission, J. Infect. Dis., 210, S674, 10.1093/infdis/jiu398 Gosmann, 2017, Lactobacillus-deficient cervicovaginal bacterial communities are associated with increased HIV acquisition in young south African women, Immunity, 46, 29, 10.1016/j.immuni.2016.12.013 Wiggins, 2001, Mucinases and sialidases: their role in the pathogenesis of sexually transmitted infections in the female genital tract, Sex. Transm. Inf., 77, 402, 10.1136/sti.77.6.402 Bertran, 2016, Slight pro-inflammatory immunomodulation properties of dendritic cells by Gardnerella vaginalis: the “Invisible Man” of bacterial vaginosis?, J. Immunol. Res, 2016, 9747480, 10.1155/2016/9747480 LeGoff, 2014, Diagnosis of genital herpes simplex virus infection in the clinical laboratory, Virol. J., 11, 10.1186/1743-422X-11-83 Zeitlin, 1996, Topically applied human recombinant monoclonal IgG1 antibody and its Fab and F(ab')2 fragments protect mice from vaginal transmission of HSV-2, Virology, 225, 213, 10.1006/viro.1996.0589 Fernandez-Romero, 2015, Multipurpose prevention technologies: the future of HIV and STI protection, Trends Microbiol, 23, 429, 10.1016/j.tim.2015.02.006 Uyangaa, 2014, Prophylactic and therapeutic modulation of innate and adaptive immunity against mucosal infection of herpes simplex virus, Immune Netw., 14, 187, 10.4110/in.2014.14.4.187 Qiu, 2014, Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp, Nature, 514, 47, 10.1038/nature13777 Roth, 2013, HSV-2 vaccine: current state and insights into development of a vaccine that targets genital mucosal protection, Microb. Pathog., 58, 45, 10.1016/j.micpath.2012.11.001 Escolano, 2017, Progress toward active or passive HIV-1 vaccination, J. Exp. Med, 214, 3, 10.1084/jem.20161765 Kelley, 2009, Industrialization of mAb production technology, mAbs, 1, 443, 10.4161/mabs.1.5.9448 Liu, 2014, Recovery and purification process development for monoclonal antibody production, mAbs, 2, 480, 10.4161/mabs.2.5.12645 O'Hare, 1999, Live-cell analysis of a green fluorescent protein-tagged herpes simplex virus infection, J. Virol., 73, 4110, 10.1128/JVI.73.5.4110-4119.1999 Apgar, 2000, Multiple-particle tracking measurements of heterogeneities in solutions of actin filaments and actin bundles, Biophys. J., 79, 1095, 10.1016/S0006-3495(00)76363-6 Lai, 2014, Efficient circular thresholding, IEEE Trans. Image Process, 23, 992, 10.1109/TIP.2013.2297014 Wang, 2015, Minimizing biases associated with tracking analysis of submicron particles in heterogeneous biological fluids, J. Control Release, 220, 37, 10.1016/j.jconrel.2015.10.021 Fadrosh, 2014, An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform, Microbiome, 2, 6, 10.1186/2049-2618-2-6