Hemolysate Pretreatment Ameliorates Ischemic Acute Renal Injury in Rats

Nephron - Tập 92 Số 2 - Trang 407-413 - 2002
Rika Yoneya1, Yoji Nagashima2, Kyohei Sakaki1, Kiyokazu Hagiwara3, Hirobumi Teraoka1, Hisashi Ozasa4, Saburo Horikawa1
1Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Tokyo
2Department of Pathology, Yokohama City University School of Medicine, Yokohama
3Division of Applied Food, The National Institute of Health and Nutrition, Tokyo, and
4Minami-Ikebukuro Clinic, Tokyo, Japan

Tóm tắt

Heme oxygenase-1 (HO-1) is an antioxidant enzyme and is believed to protect against oxidative stress-induced tissue injury. Renal ischemia-reperfusion (IR) injury seems at least in part to be caused by the oxidative stress. The aim of this study was to improve the renal IR injury by clinically available means. When littermate hemolysate was intravenously administered into rats, HO-1 was markedly induced in the kidneys. To investigate whether prior induction of HO-1 by the hemolysate injection ameliorates the subsequent renal IR injury, we assessed the levels of blood urea nitrogen (BUN) and serum creatinine (SCr), markers for renal injury, in rats with 45 min of ischemia followed by 18 h of reperfusion. To avoid the nephrotoxicity induced by hemolysate, small but effective amounts of hemolysate was injected into rats at 48 h prior to the ischemia. The levels of BUN and SCr values were significantly improved as compared to the rats with renal IR injury alone. Administration of HO inhibitor abolished the efficacy of hemolysate pretreatment. Our findings indicated that the prior induction of HO-1 by treatment of littermate hemolysate ameliorated the subsequent renal IR injury. Prior injection of self-hemolysate would be clinically useful for the protection against the renal IR injury induced by kidney transplantation and kidney surgery without immunological and infectious problems.

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Tài liệu tham khảo

10.1073/pnas.96.5.2445

10.1016/0020-711X(88)90093-6

10.1016/S0022-2143(96)90026-8

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Thông tin xuất bản

Nephron

Tập 92 Số 2

407-413

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