Halococcus hamelinensis sp. nov., a novel halophilic archaeon isolated from stromatolites in Shark Bay, Australia

International Journal of Systematic and Evolutionary Microbiology - Tập 56 Số 6 - Trang 1323-1329 - 2006
Falicia Goh1, Stefan Leuko2, Michelle A. Allen1, John P. Bowman3, Masahiro Kamekura4, Brett A. Neilan2,1, Brendan P. Burns2,1
1School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
2Australian Centre for Astrobiology, Macquarie University, Building E8C 153, Sydney, NSW 2109, Australia
3Australian Food Safety Centre, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia.
4Noda Institute for Scientific Research, 399 Noda, Noda-shi, Chiba-ken 278-0037, Japan.

Tóm tắt

Several halophilic archaea belonging to the genus Halococcus were isolated from stromatolites from Hamelin Pool, Shark Bay, Western Australia, collected during field trips in 1996 and 2002. This is the first incidence of halophilic archaea being isolated from this environment. Stromatolites are biosedimentary structures that have been formed throughout the earth's evolutionary history and have been preserved in the geological record for over 3 billion years. The stromatolites from Hamelin Pool, Western Australia, are the only known example of extant stromatolites forming in hypersaline coastal environments. Based on their 16S rRNA gene sequences and morphology, the isolates belong to the genus Halococcus. Strain 100NA1, isolated from stromatolites collected in 2002, was closely related to strain 100A6T that was isolated from the stromatolites collected in 1996, with a DNA–DNA hybridization value of 94±8 %. DNA–DNA hybridization values of strain 100A6T with Halococcus morrhuae NRC 16008 and Halococcus saccharolyticus ATCC 49257T were 17±6 and 11±7 %, respectively. The DNA G+C content of strain 100A6T was 60.5 mol% (T m). The main polar lipid was S-DGA-1, a sulphated glycolipid that has been detected in all strains of the genus Halococcus. Whole-cell protein profiles, enzyme composition and utilization of various carbon sources were distinct from those of all previously characterized Halococcus species. The recognition of this strain as representing a novel species within the genus Halococcus is justified, and the name Halococcus hamelinensis sp. nov. is proposed. The type strain is 100A6T (=JCM 12892T=ACM 5227T).

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Tài liệu tham khảo

Arp, 2001, Photosynthesis-induced biofilm calcification and calcium concentrations in Phanerozoic oceans, Science, 292, 1701, 10.1126/science.1057204

Bowman, 1998, Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats, Int J Syst Bacteriol, 48, 1213, 10.1099/00207713-48-4-1213

Burns, 2004, Microbial diversity of extant stromatolites in the hypersaline marine environment of Shark Bay, Australia, Environ Microbiol, 6, 1096, 10.1111/j.1462-2920.2004.00651.x

Byerly, 1986, Stromatolites from the 3,300–3,500-Myr Swaziland supergroup, Barberton Mountain Land, South Africa, Nature, 319, 489, 10.1038/319489a0

DasSarma, 1995, Media for halophiles, In Archaea: a Laboratory Manual, 225

DeLong, 1992, Archaea in coastal marine environments, Proc Natl Acad Sci U S A, 89, 5685, 10.1073/pnas.89.12.5685

Denner, 1994, Halococcus salifodinae sp. nov., an archaeal isolate from an Austrian salt mine, Int J Syst Bacteriol, 44, 774, 10.1099/00207713-44-4-774

Grant, 2001, Class III. Halobacteria class. nov, In Bergey's Manual of Systematic Bacteriology, vol, 294

Humble, 1977, API ZYM: a simple rapid system for the detection of bacterial enzymes, J Clin Pathol, 30, 275, 10.1136/jcp.30.3.275

Jackman, 1987, Microbial systematics based on electrophoretic whole-cell protein patterns, Methods Microbiol, 19, 209, 10.1016/S0580-9517(08)70411-2

Kamekura, 1993, Lipids of extreme halophiles, In The Biology of Halophilic Bacteria, 135

Kamekura, 1998, Diversity of extremely halophilic bacteria, Extremophiles, 2, 289, 10.1007/s007920050071

Kamekura, 2004, The potential use of signature bases from 16S rRNA gene sequences to aid the assignment of microbial strains to genera of halobacteria, In Halophilic Microorganisms, 77, 10.1007/978-3-662-07656-9_5

Kocur, 1973, Taxonomic status of the genus Halococcus Schoop, Int J Syst Bacteriol, 23, 151, 10.1099/00207713-23-2-151

Montero, 1989, Halococcus saccharolyticus sp. nov., a new species of extremely halophilic non-alkaliphilic cocci, Syst Appl Microbiol, 12, 167, 10.1016/S0723-2020(89)80010-4

Montero, 1993, DNA-rRNA hybridization studies on Halococcus saccharolyticus and other halobacteria, FEMS Microbiol Lett, 111, 69, 10.1111/j.1574-6968.1993.tb06363.x

Neilan, 2002, Molecular identification of cyanobacteria associated with stromatolites from distinct geographical locations, Astrobiology, 2, 271, 10.1089/153110702762027853

Ochsenreiter, 2002, Diversity of Archaea in hypersaline environments characterized by molecular-phylogenetic and cultivation studies, Extremophiles, 6, 267, 10.1007/s00792-001-0253-4

Oren, 1997, Proposed minimal standards for description of new taxa in the order Halobacteriales, Int J Syst Bacteriol, 47, 233, 10.1099/00207713-47-1-233

Stan-Lotter, 2002, Halococcus dombrowskii sp. nov., an archaeal isolate from a Permian alpine salt deposit, Int J Syst Evol Microbiol, 52, 1807

Vreeland, 2000, Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal, Nature, 407, 897, 10.1038/35038060

Vreeland, 2002, Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate, Extremophiles, 6, 445, 10.1007/s00792-002-0278-3

Wais, 1988, Recovery of halophilic archaebacteria from natural environments, FEMS Microbiol Ecol, 53, 211, 10.1111/j.1574-6968.1988.tb02666.x

Walter, 1980, Stromatolites 3,400–3,500 Myr old from the North Pole area, Western Australia, Nature, 284, 443, 10.1038/284443a0

Wayne, 1987, International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics, Int J Syst Bacteriol, 37, 463, 10.1099/00207713-37-4-463

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International Journal of Systematic and Evolutionary Microbiology

Tập 56 Số 6

1323-1329

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