Hairy cell sensitivity to the lysis in vitro
Tóm tắt
Experiments herein reported were designed to clarify the degree of sensitivity of hairy cells to lysis in vitro. The cytotoxic capacity of peripheral blood lymphocytes from hairy cell leukemia patients against autologous and/or allogenic hairy cells was tested both at resting conditions and after in vitro stimulation. While effectors activated by interferon α, lectins, or interleukin-2 were unable to induce a lysis of hairy cells, in some cases we were successful in eliciting a definite lysis of these cells by triggering the effector cells with anti-CD3 monoclonal antibodies. Our results favour the interpretation that hairy cells are relatively, but not completely resistant to in vitro lysis. Furthermore, the evidence that anti-CD3 antibodies increase the efficacy of the cytotoxic machinery might support the use of these molecules in designing new immunotherapeutic approaches against tumor targets.
Tài liệu tham khảo
Adler A, Chervenich PA, Whiteside T, Lotzova E, Herberman RB (1988) Interleukin 2 induction of lymphokine-activated killer (LAK) activity in the peripheral blood and bone marrow of acute leukemia patients. I. Feasibility of LAK generation in adult patients with active disease and in remission. Blood 71: 709
Anderson PM, Bach FH, Ochoa AC (1988) Augmentation of cell number and LAK activity in peripheral blood mononuclear cells activated with anti-CD3 and interleukin-2. Preliminary results in children with acute lymphoblastic leukemia and neuroblastoma. Cancer Immunol Immunother 27: 82
Cheson BD, Martin A (1987) Clinical trials in hairy cell leukemia. Current status and future directions. Ann Intern Med 106: 871
Demeter J, Beneruz M (1989) Hairy cells are not lysed by autologous LAK cells. Blut 58: 49
Fierro MT, Liao XS, Lusso P, Bonferroni M, Matera L, Cesano A, Lista P, Arione R, Forni G, Foà R (1988) In vitro and in vivo susceptibility of human leukemic cells to lymphokine activated killer activity. Leukemia 2: 50
Foà R, Fierro MT, Lusso P, Bonferroni M, Raspadori D, Buzzi M, Zinzani PL, Resegotti L, Lauria F (1987) Effect of α-Interferon on the immune system of patients with hairy cell leukemia. Leukemia 1: 377
Foon KA, Maluish AE, Abrams PG, Wrightington S, Stevenson HC, Alarif A, Fer MF, Overton WR, Poole M, Schnipper EF, Jaffe ES, Herberman RB (1986) Recombinant leukocyte A interferon therapy for advanced hairy cell leukemia. Therapeutic and immunologic results. Am J Med 80: 351
Golomb HM, Fefer A, Golde DW, Ozer H, Portlock C, Silber R, Rappaport H, Ratain MJ, Thompson J, Bonnem EM, Spiegel RJ, Tensen L, Burke JS, Vardiman JW (1987) Sequential evaluation of α-2b-interferon treatment in 128 patients with hairy cell leukemia. Sem Oncol 14: suppl. 2, 13
Griffiths SD, Cawley JC (1988) α-Interferon and lymphokineactivated killer (LAK) cells in hairy cell leukemia. Leukemia 2: 377
Lahat N, Aghai E, Kotler A, Kinarty A, Sobel E, Gruener N, Froom P (1988) Discordant effect of interferon on natural killer activity and tumor cell sensitivity to lysis in hairy cell leukemia. Blood 71: 1141
Lotzova E, Savary CA, Herberman RB (1987) Induction of NK cell activity against fresh human leukemia in culture with interleukin 2. J Immunol 138: 2718
Ochoa AC, Gromo G, Alter BJ, Sondel PM, Bach FH (1987) Long-term growth of lymphokine-activated killer (LAK) cells: role of anti-CD3, β-IL 1, interferon γ and β. J Immunol 138: 2728
Oshimi K, Oshimi Y, Yamada O, Mizoguchi H (1985) Lysis of lymphoma cells by autologous and allogenic NK cells. Blood 65: 638
Semenzato G, Pizzolo G, Agostini C, Ambrosetti A, Zambello R, Trentin L, Luca M, Masciarelli M, Chilosi M, Vinante F, Perona G, Cetto G (1986) α-Interferon activates the natural killer system in patients with hairy cell leukemia. Blood 68: 293
Semenzato G, Trentin L, Zambello R, Agostini C, Bulian P, Ambrosetti A, Vinante F, Prior M, Chilosi M, Pizzolo G. (1988) Origin of the soluble interleukin-2 receptor in the serum of patients with hairy cell leukemia. Leukemia 2: 788
Sigaux F, Chapuis F, Castaigne S, Degos L, Flandrin G, Gluckman JC (1987) Hairy cells are not lysed by NK cells. Blut 54: 319
Sung SJ, Bjorndahl JM, Wang CY, Kao HT, Fu SM (1988) Production of tumor necrosis factor/cachectin by human T cell lines and peripheral blood T lymphocytes stimulated by phorbol myristate acetate and anti-CD3 antibody. J Exp Med 167: 937
Trentin L, Ambrosetti A, Zambello R, Agostini C, Masciarelli M, Vinante F, Chilosi M, Pizzolo G, Cetto G, Semenzato G (1987) α-Interferon activated cytotoxic lymphocytes in hairy cell leukemia: evaluation of cytotoxic events. Leukemia Res 11: 843
Trentin L, Pizzolo G, Feruglio C, Zambello R, Masciarelli M, Bulian P, Agostini C, Vinante F, Zanotti R, Semenzato G (1989) Functional analysis of cytotoxic cells in patients with acute non-lymphoblastic leukemia in complete remission. Cancer 64: 667
Tovar Z, Dauphinee M, Talal N (1988) Synergistic interaction between anti-CD3 and IL-2 demonstrated by proliferative response, interferon production, and non-MHC-restricted killing. Cell Immunol 117: 12