Haematococcus pluvialis as a source of fatty acids and phytosterols: potential nutritional and biological implications

Springer Science and Business Media LLC - Tập 28 - Trang 3283-3294 - 2016
Paola G. Scodelaro Bilbao1,2,3, Cecilia Damiani1,3, Gabriela A. Salvador2,3, Patricia Leonardi1,3
1Laboratorio de Estudios Básicos y Biotecnológicos en Algas (LEBBA), Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bahía Blanca, Argentina
2Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bahía Blanca, Argentina
3Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS), Bahía Blanca, Argentina

Tóm tắt

The main aim of this work was to describe the effect of light-induced stress on bioactive lipid accumulation in Haematococcus pluvialis CCALA 1081. To this end, the lipid profile was analyzed in order to determine triacylglyceride (TAG) and phytosterol content, the fatty acid profile of TAGs and the composition of the sterol fraction. After 3 days of light-induced stress, the content of both bioactive lipids significantly increased compared to controls. Palmitic, linoleic, and α linolenic fatty acid content was higher whereas caproic acid content diminished in H. pluvialis under stress. High irradiance also reduced β-sitosterol content whereas chlerosterol, brassicasterol, and Δ7-campesterol content was higher in the phytosterol fraction. No significant levels of lipid peroxidation were detected after 3 days of light-induced stress. In addition, H. pluvialis phytosterols displayed radical scavenging capacity and also a cytotoxic effect associated with the induction of apoptosis in human IMR-32 neuroblastoma cells. Our results show that high light stress induced the accumulation of commercially valuable compounds in H. pluvialis that are of potential benefit to human health.

Tài liệu tham khảo

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