HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures

Michael Fenech1, Chang‐Chuan Chan2, Micheline Kirsch‐Volders3, Nina Holland4, Stefano Bonassi5, Errol Zeiger6
1CSIRO,
2 National Yang Ming University
3Vrije Universiteit Brussel,
4University of California at Berkeley
5Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy
6Errol Zeiger Consulting

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Fenech, 1999, The HUMN project—an international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans, Mutat. Res., 428, 271, 10.1016/S1383-5742(99)00053-8

Fenech, 2000, The in vitro micronucleus technique, Mutat. Res., 455, 81, 10.1016/S0027-5107(00)00065-8

Bonassi, 2001, The HUman MicroNucleus project. International database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes. I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei, Environ. Mol. Mutagen., 37, 31, 10.1002/1098-2280(2001)37:1<31::AID-EM1004>3.0.CO;2-P

M. Fenech, S. Bonassi, J. Turner, C. Lando, M. Ceppi, W.P. Chang, N. Holland, M. Kirsch-Volders, E. Zeiger, M.P. Bigatti, C. Bolognesi, C. Jia, G. De Luca, M. Di Giorgio, L.R. Ferguson, A. Fucic, O.G. Lima, V. Hadjidekova, P. Hrelia, A. Jaworska, G. Joksic, A.P. Krishnaja, T.K. Lee, A. Martelli, M.J. McKay, L. Migliore, E. Mirkova, W.U. Müller, Y. Odagiri, T. Orsiere, M.R. Scarfı̀, M.J. Silva, T. Sofuni, J. Suralles, G. Trenta, I. Vorobtsova, A. Vral, A. Zijno, Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Results of an international slide-scoring exercise by the HUMN project. Mutat. Res. 534 (2003) 45–64.

Fenech, 1999, Necrosis, apoptosis, cytostasis and DNA damage in human lymphocytes measured simultaneously within the cytokinesis-block micronucleus assay: description of the method and results for hydrogen peroxide, Mutagenesis, 14, 605, 10.1093/mutage/14.6.605

Kirsch-Volders, 2001, Inclusion of micronuclei in non-divided mononuclear lymphocytes and necrosis/apoptosis may provide a more comprehensive cytokinesis-block micronucleus assay for biomonitoring purposes, Mutagenesis, 19, 51, 10.1093/mutage/16.1.51

Shimizu, 1998, Selective entrapment of extrachromosomally amplified DNA by nuclear budding and micronucleation during S phase, J. Cell Biol., 140, 1307, 10.1083/jcb.140.6.1307

Shimizu, 2000, Selective elimination of acentric double minutes from cancer cells through the extrusion of micronuclei, Mutat. Res., 448, 81, 10.1016/S0027-5107(00)00003-8

Miele, 1989, The presence of amplified regions affects the stability of chromosomes in drug-resistant Chinese hamster cells, Mutat. Res., 219, 171, 10.1016/0921-8734(89)90012-X

Haaf, 1999, Sequestration of mammalian Rad51-recombination protein into micronuclei, J. Cell Biol., 144, 11, 10.1083/jcb.144.1.11

Umegaki, 2000, Cytokinesis-block micronucleus assay in WIL2-NS cells: a sensitive system to detect chromosomal damage induced by reactive oxygen species and activated human neutrophils, Mutagenesis, 15, 261, 10.1093/mutage/15.3.261