HPLC in Characterization of Hemoglobin Profile in Thalassemia Syndromes and Hemoglobinopathies: A Clinicohematological Correlation

Indian Journal of Hematology and Blood Transfusion - Tập 31 Số 1 - Trang 110-115 - 2015
Rachna Khera1, Tejinder Singh1, Nita Khuana1, Naresh Gupta2, AP Dubey3
1Department of Pathology, Maulana Azad Medical College, New Delhi, 110002, India
2Department of Medicine, Maulana Azad Medical College, New Delhi, India
3Department of Pediatrics, Nizam’s institute of Medical Sciences, Hyderabad, India

Tóm tắt

Từ khóa


Tài liệu tham khảo

Schneider RG, Alperin JB, Lehmann H (1967) Sickling tests. Pitfalls in Performance and interpretation. JAMA 202(5):419–421

Rangan A, Handoo A, Sinha S, Saxena R, Verma IC, Kumar S et al (2009) Utility of family studies in diagnosing abnormal hemoglobins/thalassemic states. Indian J Pediatr 76:615–621

Ou CN, Rognerud CL (2001) Diagnosis of hemoglobinopathies: electrophoresis vs HPLC. Clin Chim Acta 313:187–194

Riou J, Godart C, Didier H, Mathis M, Bimet C, Bardakdjian-Michau J et al (1997) Cation-exchange HPLC evaluated for presumptive identification of hemoglobin variants. Clin Chem 43:34–39

Eastman JW, Wong R, Liao CL, Morales DR (1996) Automated HPLC screening of newborns for sickle cell anemia and other hemoglobinopathies. Clin Chem 42:704–710

Eastman JW, Lorey F, Arnopp J, Currier RJ, Sherwin J, Cunningham G (1999) Distribution of hemoglobin F, A, S, C, E and D quantities in 4 million newborn screening specimens. Clin Chem 45:683–685

Mario N, Baudin B, Aussel C, Giboudeau J (1997) Capillary isoelectric focusing and high-performance cation-exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants. Clin Chem 43:2137–2142

Fucharoen S, Winichagoon P, Wisedpanichkij R, Sae-Ngow B, Sriphanich R, Oncoung W et al (1998) Prenatal and postnatal diagnoses of thalassemias and hemoglobinopathies by HPLC. Clin Chem 44:740–748

Tyagi S, Saxena R, Choudhry VP (2003) HPLC—how necessary is it for haemoglobinopathy diagnosis in India? Indian J Pathol Microbiol 46:390–393

Colah RB, Surve R, Sawant P, D’Souza E, Italia K, Phanasgaonkar S et al (2007) HPLC studies in hemoglobinopathies. Indian J Pediatr 74:657–662

Kattamis CA, Kattamis AC (1995) Management of thalassemia: growth and development, Hormone substitution, vitamin supplementation, and vaccination. Semin Hematol 32:269

Madan N, Sikka M, Sharma S, Rusia U (1998) Phenotypic expression of hemoglobin A2 in beta-thalassemia trait with iron deficiency. Ann Hematol 77(3):93–96

Nagel RL, Steinberg MH (2001) Hemoglobin SC disease and HbC disorders. In: Steinberg MH, Forget BG, Higgs DR, Nagel RL (eds) Disorders of hemoglobin: genetics, pathophysiology, and clinical management. Cambridge University Press, New York, pp 756–785

Steinberg MH (2001) Compound heterozygous and other sickle hemoglobinopathies. In: Steinberg MH, Forget BG, Higgs DR, Nagel RL (eds) Disorders of hemoglobin: genetics, pathophysiology, and clinical management. Cambridge University Press, New York, pp 786–810

Joutovsky A, Hadzi-Nesic J, Nardi MA (2004) HPLC retention time as a diagnostic tool for hemoglobin variants and hemoglobinopathies: a study of 60000 samples in a clinical diagnostic laboratory. Clin Chem 50:1736–1747