HIV-1 Detection and Subtyping by PCR and Heteroduplex Mobility Assay in Blood Donors: Can These Tests Help to Elucidate Conflicting Serological Results?

Constantine, 1993, Serologic tests for the retroviruses: approaching a decade of evolution, AIDS, 7, 1, 10.1097/00002030-199301000-00001 Lee, 1991, Quantitative assessment of HIV-1 DNA load by coamplification of HIV-1 gag and HLA-DQ-α genes, AIDS, 5, 683, 10.1097/00002030-199106000-00007 Myers G, Korber B, Wain-Robson S, Smith RF, Berzofsky JA, Pavlakis GN: Human Retroviruses and AIDS 1993. Los Alamos, NM, Los Alamos National Laboratory, 1993. Kostrikis, 1995, Genetic analysis of human immunodeficiency virus type 1 strains from patients in Cyprus: identification of a new subtype I, J Virol, 69, 6122, 10.1128/jvi.69.10.6122-6130.1995 Sharp, 1994, Origins and diversity of human immunodeficiency viruses, AIDS, 8, S27 Delwart, 1993, A DNA heteroduplex gel shift assay for the analysis of HIV sequence diversity, Science, 262, 1257, 10.1126/science.8235655 Delwart EL, Shpaer EG, Mullins JI: Heteroduplex mobility assays for phylogenetic analysis, in Innis, MA, Gelfard, DH, Sninsky, JJ. (eds): PCR Strategies 1995. New York, Academic Press, 1995. Delwart, 1995, Genetic subtyping of immunodeficiency virus using a heteroduplex mobility assay, PCR Methods and Applications, 4, 5202, 10.1101/gr.4.5.S202 Bachmann, 1994, Rapid genetic characterization of HIV type 1 strains from four WHO-sponsored vaccine evaluation sites using a heteroduplex mobility assay, AIDS ResaHum Retroviruses, 10, 1345, 10.1089/aid.1994.10.1345 World Health Organisation: Guidelines for Standard HIV Isolation Procedures. WHO, Genève, 1995. Menitove, 1996, Transfusion-transmitted infections: update, Sem Hematol, 33, 290 Knüver-Hopf, 1993, Blood donations indeterminate in HIV-1 Western blot analysed by IgM immunoblot and polymerase chain reaction, Vox Sang, 64, 89, 10.1159/000462317 Perrin, 1990, Human immunodeficiency virus DNA amplification and serology in blood donors, Blood, 3, 641, 10.1182/blood.V76.3.641.641