Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe

Parasites and Vectors - Tập 8 Số 1 - Trang 75 - 2015
Ángel Sainz1, Xavier Roura2, Guadalupe Miró3, Agustı́n Estrada-Peña4, Barbara Kohn5, Shimon Harrus6, Laia Solano‐Gallego7
1Department of Animal Medicine and Surgery, Veterinary Clinic Hospital, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, Madrid, Spain
2Hospital Clinic Veterinari, Universitat Autònoma de Barcelona, Barcelona, Spain
3Department of Animal Health, Veterinary Clinic Hospital, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, Madrid, Spain
4Department of Animal Pathology, Faculty of Veterinary Medicine, University of Zaragoza, Zaragoza, Spain
5Small Animal Clinic, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany
6Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel
7Departament de Medicina i Cirurgia Animal, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Bellaterra, Spain

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Donatien A, Lestoquard F. Existence en Algerie d’une Rickettsia du chien. Bull Soc Pathol Exot. 1935;28:418–9.

Huxsoll DL, Hildebrandt PK, Nims RM, Walker JS. Tropical canine pancytopenia. J Am Vet Med Assoc. 1970;157(11):1627–32.

Harvey JW, Simpson CF, Gaskin JM. Cyclic thrombocytopenia induced by a Rickettsia-like agent in dogs. J Infect Dis. 1978;137(2):182–8.

Madewell BR, Gribble DH. Infection in two dogs with an agent resembling Ehrlichia equi. J Am Vet Med Assoc. 1982;180(5):512–4.

Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, et al. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol. 2001;51(Pt 6):2145–65.

Neer TM, Breitschwerdt EB, Greene RT, Lappin MR. Consensus statement on ehrlichial disease of small animals from the infectious disease study group of the ACVIM. American College of Veterinary Internal Medicine. J Vet Intern Med. 2002;16(3):309–15.

Keysary A, Waner T, Rosner M, Warner CK, Dawson JE, Zass R, et al. The first isolation, in vitro propagation, and genetic characterization of Ehrlichia canis in Israel. Vet Parasitol. 1996;62(3–4):331–40.

Aguirre E, Sainz A, Dunner S, Amusategui I, Lopez L, Rodriguez-Franco F, et al. First isolation and molecular characterization of Ehrlichia canis in Spain. Vet Parasitol. 2004;125(3–4):365–72.

Shpynov SN, Fournier PE, Rudakov NV, Samoilenko IE, Reshetnikova TA, Yastrebov VK, et al. Molecular identification of a collection of spotted Fever group rickettsiae obtained from patients and ticks from Russia. Am J Trop Med Hyg. 2006;74(3):440–3.

Spitalska E, Boldis V, Kostanova Z, Kocianova E, Stefanidesova K. Incidence of various tick-borne microorganisms in rodents and ticks of central Slovakia. Acta Virol. 2008;52(3):175–9.

Dongus H, Zahler M. Gothe R: [The brown dog tick, Rhipicephalus sanguineus (Ixodidae), in Germany: an epidemiologic study and control measures]. Berl Munch Tierarztl Wochenschr. 1996;109(6–7):245–8.

Gothe R. [Ehrlichia canis infections of dogs in Germany. Epidemiology, diagnosis, therapy and prophylaxis]. Tierarztl Prax Ausg K Kleintiere Heimtiere. 1998;26(6):396–401.

Pusterla N, Pusterla JB, Deplazes P, Wolfensberger C, Muller W, Horauf A, et al. Seroprevalence of Ehrlichia canis and of canine granulocytic Ehrlichia infection in dogs in Switzerland. J Clin Microbiol. 1998;36(12):3460–2.

Hamel D, Silaghi C, Knaus M, Visser M, Kusi I, Rapti D, et al. Detection of Babesia canis subspecies and other arthropod-borne diseases in dogs from Tirana, Albania. Wien Klin Wochenschr. 2009;121 Suppl 3:42–5.

Perez Vera C, Kapiainen S, Junnikkala S, Aaltonen K, Spillmann T, Vapalahti O. Survey of selected tick-borne diseases in dogs in Finland. Parasit Vectors. 2014;7:285.

Pantchev N, Schaper R, Limousin S, Norden N, Weise M, Lorentzen L. Occurrence of Dirofilaria immitis and tick-borne infections caused by Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Ehrlichia canis in domestic dogs in France: results of a countrywide serologic survey. Parasitol Res. 2009;105 Suppl 1:S101–14.

Hamel D, Silaghi C, Pfister K. Arthropod-borne infections in travelled dogs in Europe. Parasite. 2013;20:9.

Hamel D, Rohrig E, Pfister K. Canine vector-borne disease in travelled dogs in Germany–a retrospective evaluation of laboratory data from the years 2004–2008. Vet Parasitol. 2011;181(1):31–6.

Menn B, Lorentz S, Naucke TJ. Imported and travelling dogs as carriers of canine vector-borne pathogens in Germany. Parasit Vectors. 2010;3:34.

Farkas R, Gyurkovszky M, Lukacs Z, Aladics B, Solymosi N. Seroprevalence of some vector-borne infections of dogs in Hungary. Vector Borne Zoonotic Dis. 2014;14(4):256–60.

Pennisi MG, Capri A, Solano-Gallego L, Lombardo G, Torina A, Masucci M. Prevalence of antibodies against Rickettsia conorii, Babesia canis, Ehrlichia canis, and Anaplasma phagocytophilum antigens in dogs from the Stretto di Messina area (Italy). Ticks Tick Borne Dis. 2012;3(5–6):315–8.

Trotta M, Fogliazza A, Furlanello T, Solano-Gallego L. A molecular and serological study of exposure to tick-borne pathogens in sick dogs from Italy. Clin Microbiol Infect. 2009;15 Suppl 2:62–3.

Torina A, Vicente J, Alongi A, Scimeca S, Turla R, Nicosia S, et al. Observed prevalence of tick-borne pathogens in domestic animals in Sicily, Italy during 2003-2005. Zoonoses Public Health. 2007;54(1):8–15.

Corrain R, Di Francesco A, Bolognini M, Ciucci P, Baldelli R, Guberti V. Serosurvey for CPV-2, distemper virus, ehrlichiosis and leishmaniosis in free-ranging dogs in Italy. Vet Rec. 2007;160(3):91–2.

Solano-Gallego L, Trotta M, Razia L, Furlanello T, Caldin M. Molecular survey of Ehrlichia canis and Anaplasma phagocytophilum from blood of dogs in Italy. Ann N Y Acad Sci. 2006;1078:515–8.

Cocco R, Sanna G, Cillara MG, Tola S, Ximenes L, Pinnaparpaglia ML, et al. Ehrlichiosis and rickettsiosis in a canine population of Northern Sardinia. Ann N Y Acad Sci. 2003;990:126–30.

Buonavoglia D, Sagazio P, Gravino EA, De Caprariis D, Cerundolo R, Buonavoglia C. Serological evidence of Ehrlichia canis in dogs in southern Italy. New Microbiol. 1995;18(1):83–6.

Cardoso L, Mendao C. Madeira de Carvalho L: Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in apparently healthy and CVBD-suspect dogs in Portugal--a national serological stud. Parasit Vectors. 2012;5:62.

Alexandre N, Santos AS, Nuncio MS, Sousa R, Boinas F, Bacellar F. Detection of Ehrlichia canis by polymerase chain reaction in dogs from Portugal. Vet J. 2009;181(3):343–4.

Bacellar F, Dawson JE, Silveira CA, Filipe AR. Antibodies against Rickettsiaceae in dogs of Setubal. Portugal Cent Eur J Public Health. 1995;3(2):100–2.

Mircean V, Dumitrache MO, Gyorke A, Pantchev N, Jodies R, Mihalca AD, et al. Seroprevalence and geographic distribution of Dirofilaria immitis and tick-borne infections (Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Ehrlichia canis) in dogs from Romania. Vector Borne Zoonotic Dis. 2012;12(7):595–604.

Volgina NS, Romashov BV, Romashova NB, Shtannikov AV. Prevalence of borreliosis, anaplasmosis, ehrlichiosis and Dirofilaria immitis in dogs and vectors in Voronezh Reserve (Russia). Comp Immunol Microbiol Infect Dis. 2013;36(6):567–74.

Miro G, Montoya A, Roura X, Galvez R, Sainz A. Seropositivity rates for agents of canine vector-borne diseases in Spain: a multicentre study. Parasit Vectors. 2013;6:117.

Couto CG, Lorentzen L, Beall MJ, Shields J, Bertolone N, Couto JI, et al. Serological study of selected vector-borne diseases in shelter dogs in central Spain using point-of-care assays. Vector Borne Zoonotic Dis. 2010;10(9):885–8.

Tabar MD, Francino O, Altet L, Sanchez A, Ferrer L, Roura X. PCR survey of vectorborne pathogens in dogs living in and around Barcelona, an area endemic for leishmaniasis. Vet Rec. 2009;164(4):112–6.

Amusategui I, Tesouro MA, Kakoma I, Sainz A. Serological reactivity to Ehrlichia canis, Anaplasma phagocytophilum, Neorickettsia risticii, Borrelia burgdorferi and Rickettsia conorii in dogs from northwestern Spain. Vector Borne Zoonotic Dis. 2008;8(6):797–803.

Solano-Gallego L, Llull J, Osso M, Hegarty B, Breitschwerdt E. A serological study of exposure to arthropod-borne pathogens in dogs from northeastern Spain. Vet Res. 2006;37(2):231–44.

Sainz A, Amusategui I, Tesouro MA. Canine ehrlichiosis in the Comunidad de Madrid in central Spain. Ann N Y Acad Sci. 1998;849:438–40.

Sainz A, Delgado S, Amusategui I, Tesouro MA, Carmenes P. Seroprevalence of canine ehrlichiosis in Castilla-Leon north-west Spain. Prev Vet Med. 1996;29(1):1–7.

Batmaz H, Nevo E, Waner T, Senturk S, Yilmaz Z, Harrus S. Seroprevalence of Ehrlichia canis antibodies among dogs in Turkey. Vet Rec. 2001;148(21):665–6.

Kirtz B, Czettel B, Thum D, et al. Anaplasma phagozytophilum in einer österreichischen Hundepopulation: eine Prävalenz-Studie (2001-2006). Kleintierpraxis. 2007;52:562–8.

Barutzki D, De Nicola A, Zeziola M. Reule M: [Seroprevalence of Anaplasma phagocytophilum infection in dogs in Germany]. Berl Munch Tierarztl Wochenschr. 2006;119(7–8):342–7.

Jensen J, Simon D, Murua Escobar H, Soller JT, Bullerdiek J, Beelitz P, et al. Anaplasma phagocytophilum in dogs in Germany. Zoonoses Public Health. 2007;54(2):94–101.

Schaarschmidt-Kiener D, Müller W. Labordiagnostische und klinische Aspekte der caninen Anaplasmose und Ehrlichiose. Tierarztl Prax. 2007;35:129–36.

Krupka I, Pantchev N, Lorentzen L, Weise M, Straubinger RK. Tick-transmitted, bacterial infections in dogs: seroprevalences of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Ehrlichia canis in Germany. Prakt Tierarzt. 2007;88(10):776–88.

Kohn B, Silaghi C, Galke D, Arndt G, Pfister K. Infections with Anaplasma phagocytophilum in dogs in Germany. Res Vet Sci. 2011;91(1):71–6.

Barth C, Straubinger RK, Sauter-Louis C, Hartmann K. Prevalence of antibodies against Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum and their clinical relevance in dogs in Munich, Germany. Berl Munch Tierarztl Wochenschr. 2012;125(7–8):337–44.

de la Fuente J, Torina A, Naranjo V, Nicosia S, Alongi A, La Mantia F, et al. Molecular characterization of Anaplasma platys strains from dogs in Sicily, Italy. BMC Vet Res. 2006;2:24.

Torina A, Caracappa S. Dog tick-borne diseases in Sicily. Parassitologia. 2006;48(1–2):145–7.

Ebani V, Cerri D, Fratini F, Ampola M, Andreani E. Seroprevalence of Anaplasma phagocytophilum in domestic and wild animals from central Italy. New Microbiol. 2008;31(3):371–5.

Ebani VV, Bertelloni F, Turchi B, Cerri D. Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs. Ann Agric Environ Med. 2013;20(2):289–92.

Berzina I, Matise I. Seroprevalence against Borrelia burgdorferi sensu lato and occurence of antibody co-expression with Anaplasma phagocytophilum in dogs in Latvia. Ir Vet J. 2013;66(1):9.

Skotarczak B, Adamska M, Rymaszewska A, Supron M, Sawczuk M. Maciejewska A: [Anaplasma phagocytophila and protozoans of Babesia genus in dogs from endemic areas of Lyme disease in north-western Poland]. Wiad Parazytol. 2004;50(3):555–61.

Santos AS, Alexandre N, Sousa R, Nuncio MS, Bacellar F, Dumler JS. Serological and molecular survey of Anaplasma species infection in dogs with suspected tickborne disease in Portugal. Vet Rec. 2009;164(6):168–71.

Egenvall A, Bonnett BN, Gunnarsson A, Hedhammar A, Shoukri M, Bornstein S, et al. Sero-prevalence of granulocytic Ehrlichia spp. and Borrelia burgdorferi sensu lato in Swedish dogs 1991-94. Scand J Infect Dis. 2000;32(1):19–25.

Jaderlund KH, Egenvall A, Bergstrom K, Hedhammar A. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in dogs with neurological signs. Vet Rec. 2007;160(24):825–31.

Shaw SE, Binns SH, Birtles RJ, Day MJ, Smithson R, Kenny MJ. Molecular evidence of tick-transmitted infections in dogs and cats in the United Kingdom. Vet Rec. 2005;157(21):645–8.

Aguirre E, Tesouro MA, Ruiz L, Amusategui I, Sainz A. Genetic characterization of Anaplasma (Ehrlichia) platys in dogs in Spain. J Vet Med B Infect Dis Vet Public Health. 2006;53(4):197–200.

Cardoso L, Tuna J, Vieira L, Yisaschar-Mekuzas Y, Baneth G. Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from the North of Portugal. Vet J. 2010;183(2):232–3.

Beaufils JP, Inokuma H, Martin-Granel J, Jumelle P, Barbault-Jumelle M, Brouqui P. Anaplasma platys (Ehrlichia platys) infection in a dog in France: description of the case, and characterization of the agent. Rev Med Vet. 2002;153:85–90.

Ulutas B, Bayramli G, Karangec T. First case of Anaplasma (Ehrlichia) platys infection in a dog in Turkey. Turk J Vet Anim Sci. 2007;31:279–82.

Kontos VI, Papadopoulos O, French TW. Natural and experimental canine infections with a Greek strain of Ehrlichia platys. Vet Clin Pathol. 1991;20(4):101–5.

Dyachenko V, Pantchev N, Balzer HJ, Meyersen A, Straubinger RK. First case of Anaplasma platys infection in a dog from Croatia. Parasit Vectors. 2012;5:49.

Andersson M, Turcitu MA, Stefanache M, Tamba P, Barbuceanu F, Chitimia L. First evidence of Anaplasma platys and Hepatozoon canis co-infection in a dog from Romania–a case report. Ticks Tick Borne Dis. 2013;4(4):317–9.

Ramos RA, Latrofa MS, Giannelli A, Lacasella V, Campbell BE, Dantas-Torres F, et al. Detection of Anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real-time PCR. Vet Parasitol. 2014;205(1-2):285–8.

de Caprariis D, Dantas-Torres F, Capelli G, Mencke N, Stanneck D, Breitschwerdt EB, et al. Evolution of clinical, haematological and biochemical findings in young dogs naturally infected by vector-borne pathogens. Vet Microbiol. 2011;149(1–2):206–12.

Otranto D, de Caprariis D, Lia RP, Tarallo V, Lorusso V, Testini G, et al. Prevention of endemic canine vector-borne diseases using imidacloprid 10% and permethrin 50% in young dogs: a longitudinal field study. Vet Parasitol. 2010;172(3–4):323–32.

Sparagano OA, de Vos AP, Paoletti B, Camma C, de Santis P, Otranto D, et al. Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. J Vet Diagn Invest. 2003;15(6):527–34.

Groves MG, Dennis GL, Amyx HL, Huxsoll DL. Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus). Am J Vet Res. 1975;36(7):937–40.

Smith RD, Sells DM, Stephenson EH, Ristic MR, Huxsoll DL. Development of Ehrlichia canis, causative agent of canine ehrlichiosis, in the tick Rhipicephalus sanguineus and its differentiation from a symbiotic Rickettsia. Am J Vet Res. 1976;37(2):119–26.

Fourie JJ, Stanneck D, Luus HG, Beugnet F, Wijnveld M, Jongejan F. Transmission of Ehrlichia canis by Rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes. Vet Parasitol. 2013;197(3–4):595–603.

Gray J, Dantas-Torres F, Estrada-Pena A, Levin M. Systematics and ecology of the brown dog tick, Rhipicephalus sanguineus. Ticks Tick Borne Dis. 2013;4(3):171–80.

Gray JS, Dautel H, Estrada-Pena A, Kahl O, Lindgren E. Effects of climate change on ticks and tick-borne diseases in europe. Interdiscip Perspect Infect Dis. 2009;2009:593232.

Uspensky I, Ioffe-Uspensky I. The dog factor in brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) infestations in and near human dwellings. Int J Med Microbiol. 2002;291 Suppl 33:156–63.

Jaenson TG, Jaenson DG, Eisen L, Petersson E, Lindgren E. Changes in the geographical distribution and abundance of the tick Ixodes ricinus during the past 30 years in Sweden. Parasit Vectors. 2012;5:8.

Medlock JM, Hansford KM, Bormane A, Derdakova M, Estrada-Pena A, George JC, et al. Driving forces for changes in geographical distribution of Ixodes ricinus ticks in Europe. Parasit Vectors. 2013;6:1.

Estrada-Pena A, Farkas R, Jaenson TG, Koenen F, Madder M, Pascucci I, et al. Association of environmental traits with the geographic ranges of ticks (Acari: Ixodidae) of medical and veterinary importance in the western Palearctic. A digital data set. Exp Appl Acarol. 2013;59(3):351–66.

Yasyukevich VV, Kazakova EV, Popov IO, Semenov SM. Distribution of Ixodes ricinus L., 1758 and Ixodes persulcatus Shulze, 1930 (Parasitoformes, Ixodidae) in Russia and adjacent countries in view of observable climate changes. Dokl Earth Sci. 2009;427(2):1030–4.

Ecker M, Allison SL, Meixner T, Heinz FX. Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia. J Gen Virol. 1999;80(Pt 1):179–85.

Alekseev AN, Dubinina HV, Van De Pol I, Schouls LM. Identification of Ehrlichia spp. and Borrelia burgdorferi in Ixodes ticks in the Baltic regions of Russia. J Clin Microbiol. 2001;39(6):2237–42.

Hamel D, Silaghi C, Zapadynska S, Kudrin A, Pfister K. Vector-borne pathogens in ticks and EDTA-blood samples collected from client-owned dogs, Kiev. Ukraine Ticks Tick Borne Dis. 2013;4(1–2):152–5.

Movila A, Toderas I, Uspenskaia I, Conovalov J. Molecular detection of tick-borne pathogens in Ixodes ricinus from Moldova collected in 1960. Ticks Tick Borne Dis. 2013;4(4):359–61.

Simpson RM, Gaunt SD, Hair JA, Kocan KM, Henk WG, Casey HW. Evaluation of Rhipicephalus sanguineus as a potential biologic vector of Ehrlichia platys. Am J Vet Res. 1991;52(9):1537–41.

Sanogo YO, Davoust B, Inokuma H, Camicas JL, Parola P, Brouqui P. First evidence of Anaplasma platys in Rhipicephalus sanguineus (Acari: Ixodida) collected from dogs in Africa. Onderstepoort J Vet Res. 2003;70(3):205–12.

Johnson EM, Ewing SA, Barker RW, Fox JC, Crow DW, Kocan KM. Experimental transmission of Ehrlichia canis (Rickettsiales: Ehrlichieae) by Dermacentor variabilis (Acari: Ixodidae). Vet Parasitol. 1998;74(2–4):277–88.

McQuiston JH, Childs JE, Chamberland ME, Tabor E. Transmission of tick-borne agents of disease by blood transfusion: a review of known and potential risks in the United States. Transfusion. 2000;40(3):274–84.

Egenvall A, Bjoersdorff A, Lilliehook I, Olsson Engvall E, Karlstam E, Artursson K, et al. Early manifestations of granulocytic ehrlichiosis in dogs inoculated experimentally with a Swedish Ehrlichia species isolate. Vet Rec. 1998;143(15):412–7.

Wardrop KJ, Reine N, Birkenheuer A, Hale A, Hohenhaus A, Crawford C, et al. Canine and feline blood donor screening for infectious disease. J Vet Intern Med. 2005;19(1):135–42.

Kohn B. Anaplasma phagocytophilum in the dog – update on epidemiology and clinical disease. In: Canine vector-borne disease world forum. New York: Bayer HealthCare Animal Health; 2010. p. 36–43. 12-15 April.

Gaunt S, Beall M, Stillman B, Lorentzen L, Diniz P, Chandrashekar R, et al. Experimental infection and co-infection of dogs with Anaplasma platys and Ehrlichia canis: hematologic, serologic and molecular findings. Parasit Vectors. 2010;3(1):33.

Ebani VV, Verin R, Fratini F, Poli A, Cerri D. Molecular survey of Anaplasma phagocytophilum and Ehrlichia canis in red foxes (Vulpes vulpes) from central Italy. J Wildl Dis. 2011;47(3):699–703.

Fishman Z, Gonen L, Harrus S, Strauss-Ayali D, King R, Baneth G. A serosurvey of Hepatozoon canis and Ehrlichia canis antibodies in wild red foxes (Vulpes vulpes) from Israel. Vet Parasitol. 2004;119(1):21–6.

Torina A, Blanda V, Antoci F, Scimeca S, D’Agostino R, Scariano E, et al. A Molecular survey of Anaplasma spp., Rickettsia spp., Ehrlichia canis and Babesia microti in foxes and fleas from Sicily. Transbound Emerg Dis. 2013;60 Suppl 2:125–30.

Breitschwerdt EB, Abrams-Ogg AC, Lappin MR, Bienzle D, Hancock SI, Cowan SM, et al. Molecular evidence supporting Ehrlichia canis-like infection in cats. J Vet Intern Med. 2002;16(6):642–9.

Andre MR, Adania CH, Machado RZ, Allegretti SM, Felippe PA, Silva KF, et al. Molecular and serologic detection of Ehrlichia spp. in endangered Brazilian wild captive felids. J Wildl Dis. 2010;46(3):1017–23.

Braga Mdo S, Andre MR, Freschi CR, Teixeira MC, Machado RZ. Molecular and serological detection of Ehrlichia spp. in cats on Sao Luis Island, Maranhao, Brazil. Rev Bras Parasitol Vet. 2012;21(1):37–41.

Maia C, Ramos C, Coimbra M, Bastos F, Martins A, Pinto P, et al. Bacterial and protozoal agents of feline vector-borne diseases in domestic and stray cats from southern Portugal. Parasit Vectors. 2014;7:115.

Groen J, Koraka P, Nur YA, Avsic-Zupanc T, Goessens WH, Ott A, et al. Serologic evidence of ehrlichiosis among humans and wild animals in The Netherlands. Eur J Clin Microbiol Infect Dis. 2002;21(1):46–9.

Ismail N, Bloch KC, McBride JW. Human ehrlichiosis and anaplasmosis. Clin Lab Med. 2010;30(1):261–92.

Nicholson WL, Allen KE, McQuiston JH, Breitschwerdt EB, Little SE. The increasing recognition of rickettsial pathogens in dogs and people. Trends Parasitol. 2010;26(4):205–12.

Lima ML, Soares PT, Ramos CA, Araujo FR, Ramos RA, Souza II, et al. Molecular detection of Anaplasma platys in a naturally-infected cat in Brazil. Braz J Microbiol. 2010;41(2):381–5.

Qurollo BA, Balakrishnan N, Cannon CZ, Maggi RG, Breitschwerdt EB. Co-infection with Anaplasma platys, Bartonella henselae, Bartonella koehlerae and ‘Candidatus Mycoplasma haemominutum’ in a cat diagnosed with splenic plasmacytosis and multiple myeloma. J Feline Med Surg. 2014;16(8):713–20.

Perez M, Rikihisa Y, Wen B. Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J Clin Microbiol. 1996;34(9):2133–9.

Fishbein DB, Dawson JE, Robinson LE. Human ehrlichiosis in the United States, 1985 to 1990. Ann Intern Med. 1994;120(9):736–43.

Ganguly S, Mukhopadhayay SK. Tick-borne ehrlichiosis infection in human beings. J Vector Borne Dis. 2008;45(4):273–80.

Bakken JS, Dumler JS, Chen SM, Eckman MR, Van Etta LL, Walker DH. Human granulocytic ehrlichiosis in the upper Midwest United States. A new species emerging? JAMA. 1994;272(3):212–8.

Parola P, Davoust B, Raoult D. Tick- and flea-borne rickettsial emerging zoonoses. Vet Res. 2005;36(3):469–92.

Dumler JS, Choi KS, Garcia-Garcia JC, Barat NS, Scorpio DG, Garyu JW, et al. Human granulocytic anaplasmosis and Anaplasma phagocytophilum. Emerg Infect Dis. 2005;11(12):1828–34.

Maggi RG, Mascarelli PE, Havenga LN, Naidoo V, Breitschwerdt EB. Co-infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian. Parasit Vectors. 2013;6:103.

Arraga-Alvarado CM, Qurollo B, Parra OC, Berrueta MA, Hegarty BC, Breitschwerdt EB. Molecular Evidence of Anaplasma platys Infection in Two Women from Venezuela. Am J Trop Med Hyg. 2014;91(6):1161–5.

Buller RS, Arens M, Hmiel SP, Paddock CD, Sumner JW, Rikhisa Y, et al. Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. N Engl J Med. 1999;341(3):148–55.

Allsopp MT, Louw M, Meyer EC. Ehrlichia ruminantium: an emerging human pathogen? Ann N Y Acad Sci. 2005;1063:358–60.

Allsopp MT, Allsopp BA. Novel Ehrlichia genotype detected in dogs in South Africa. J Clin Microbiol. 2001;39(11):4204–7.

Gutierrez CN, Martinez M, Sanchez E, De Vera M, Rojas M, Ruiz J, et al. Cultivation and molecular identification of Ehrlichia canis and Ehrlichia chaffeensis from a naturally co-infected dog in Venezuela. Vet Clin Pathol. 2008;37(3):258–65.

Beall MJ, Alleman AR, Breitschwerdt EB, Cohn LA, Couto CG, Dryden MW, et al. Seroprevalence of Ehrlichia canis, Ehrlichia chaffeensis and Ehrlichia ewingii in dogs in North America. Parasit Vectors. 2012;5:29.

Ndip LM, Ndip RN, Esemu SN, Walker DH, McBride JW. Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe. Cameroon Exp Appl Acarol. 2010;50(2):163–8.

Pritt BS, Sloan LM, Johnson DK, Munderloh UG, Paskewitz SM, McElroy KM, et al. Emergence of a new pathogenic Ehrlichia species, Wisconsin and Minnesota, 2009. N Engl J Med. 2011;365(5):422–9.

Hegarty BC, Maggi RG, Koskinen P, Beall MJ, Eberts M, Chandrashekar R, et al. Ehrlichia muris infection in a dog from Minnesota. J Vet Intern Med. 2012;26(5):1217–20.

Nyindo M, Huxsoll DL, Ristic M, Kakoma I, Brown JL, Carson CA, et al. Cell-mediated and humoral immune responses of German Shepherd Dogs and Beagles to experimental infection with Ehrlichia canis. Am J Vet Res. 1980;41(2):250–4.

Harrus S, Kass PH, Klement E, Waner T. Canine monocytic ehrlichiosis: a retrospective study of 100 cases, and an epidemiological investigation of prognostic indicators for the disease. Vet Rec. 1997;141(14):360–3.

Costa Jr LM, Rembeck K, Ribeiro MF, Beelitz P, Pfister K, Passos LM. Sero-prevalence and risk indicators for canine ehrlichiosis in three rural areas of Brazil. Vet J. 2007;174(3):673–6.

Rodriguez-Vivas RI, Albornoz RE, Bolio GM. Ehrlichia canis in dogs in Yucatan, Mexico: seroprevalence, prevalence of infection and associated factors. Vet Parasitol. 2005;127(1):75–9.

Watanabe M, Okuda M, Tsuji M, Inokuma H. Seroepidemiological study of canine ehrlichial infections in Yamaguchi prefecture and surrounding areas of Japan. Vet Parasitol. 2004;124(1–2):101–7.

Egenvall AE, Hedhammar AA, Bjoersdorff AI. Clinical features and serology of 14 dogs affected by granulocytic ehrlichiosis in Sweden. Vet Rec. 1997;140(9):222–6.

McClure JC, Crothers ML, Schaefer JJ, Stanley PD, Needham GR, Ewing SA, et al. Efficacy of a doxycycline treatment regimen initiated during three different phases of experimental ehrlichiosis. Antimicrob Agents Chemother. 2010;54(12):5012–20.

Harrus S, Waner T, Aizenberg I, Foley JE, Poland AM, Bark H. Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. J Clin Microbiol. 1998;36(1):73–6.

Waner T, Harrus S, Bark H, Bogin E, Avidar Y, Keysary A. Characterization of the subclinical phase of canine ehrlichiosis in experimentally infected beagle dogs. Vet Parasitol. 1997;69(3–4):307–17.

Mylonakis ME, Koutinas AF, Breitschwerdt EB, Hegarty BC, Billinis CD, Leontides LS, et al. Chronic canine ehrlichiosis (Ehrlichia canis): a retrospective study of 19 natural cases. J Am Anim Hosp Assoc. 2004;40(3):174–84.

Egenvall A, Lilliehook I, Bjoersdorff A, Engvall EO, Karlstam E, Artursson K, et al. Detection of granulocytic Ehrlichia species DNA by PCR in persistently infected dogs. Vet Rec. 2000;146(7):186–90.

Gaunt SD, Baker DC, Babin SS. Platelet aggregation studies in dogs with acute Ehrlichia platys infection. Am J Vet Res. 1990;51(2):290–3.

Harvey JW. Thrombocytotrophic anaplasmosis (A.platys [E.platys] infection). In: Greene CG, editor. Infectious diseases of the dog and cat. 3rd ed. St. Louis: Saunders Elsevier; 2006. p. 229–31.

Greig B, Asanovich KM, Armstrong PJ, Dumler JS. Geographic, clinical, serologic, and molecular evidence of granulocytic ehrlichiosis, a likely zoonotic disease, in Minnesota and Wisconsin dogs. J Clin Microbiol. 1996;34(1):44–8.

Varela F, Font X, Valladares JE, Alberola J. Thrombocytopathia and light-chain proteinuria in a dog naturally infected with Ehrlichia canis. J Vet Intern Med. 1997;11(5):309–11.

Breitschwerdt EB, Hegarty BC, Hancock SI. Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii. J Clin Microbiol. 1998;36(9):2645–51.

Goldman EE, Breitschwerdt EB, Grindem CB, Hegarty BC, Walls JJ, Dumler JS. Granulocytic ehrlichiosis in dogs from North Carolina and Virginia. J Vet Intern Med. 1998;12(2):61–70.

Lilliehöök I, Egenvall A, Twedten HW. Hematopathology in dogs experimentally infected with a Swedish granulocytic Ehrlichia species. Vet Clin Pathol. 1998;27(4):116–22.

Neer TM. Canine monocytic and granulocytic ehrlichiosis. In: Greene CE, editor. Infectious Diseases of the Dog and Cat. 2nd ed. Philadelphia: W.B. Saunders Co; 1998. p. 139–47.

Breitschwerdt EB. Obligate intracellular pathogens. In: Ettinger SJ, Feldman EC, editors. Textbook of Veterinary Internal Medicine. 6th ed. Philadelphia, PA: W.B. Saunders Co; 2005. p. 631–2.

Komnenou AA, Mylonakis ME, Kouti V, Tendoma L, Leontides L, Skountzou E, et al. Ocular manifestations of natural canine monocytic ehrlichiosis (Ehrlichia canis): a retrospective study of 90 cases. Vet Ophthalmol. 2007;10(3):137–42.

Leiva M, Naranjo C, Pena MT. Ocular signs of canine monocytic ehrlichiosis: a retrospective study in dogs from Barcelona. Spain Vet Ophthalmol. 2005;8(6):387–93.

Diniz PP, de Morais HS, Breitschwerdt EB, Schwartz DS. Serum cardiac troponin I concentration in dogs with ehrlichiosis. J Vet Intern Med. 2008;22(5):1136–43.

Little SE. Ehrlichiosis and anaplasmosis in dogs and cats. Vet Clin North Am Small Anim Pract. 2010;40(6):1121–40.

Bellah JR, Shull RM, Selcer EV. Ehrlichia canis-related polyarthritis in a dog. J Am Vet Med Assoc. 1986;189(8):922–3.

Cowell RL, Tyler RD, Clinkenbeard KD, Meinkoth JH. Ehrlichiosis and polyarthritis in three dogs. J Am Vet Med Assoc. 1988;192(8):1093–5.

Greig B, Armstrong PJ. Canine granulocytotrophic anaplasmosis (A.phagocytophilum infection). In: Greene CG, editor. Infectious diseases of the dog and cat. 3rd ed. St. Louis: Saunders Elsevier; 2006. p. 219–24.

Alleman R, Heather W: An update on anaplasmosis in dogs. Vet Med 2008, April:212-220.

Kohn B, Galke D, Beelitz P, Pfister K. Clinical features of canine granulocytic anaplasmosis in 18 naturally infected dogs. J Vet Intern Med. 2008;22(6):1289–95.

Granick JL, Armstrong PJ, Bender JB. Anaplasma phagocytophilum infection in dogs: 34 cases (2000-2007). J Am Vet Med Assoc. 2009;234(12):1559–65.

Ravnik U, Tozon N, Strasek K, Avsic Zupanc T. Clinical and haematological features in Anaplasma phagocytophilum seropositive dogs. Clin Microbiol Infect. 2009;15 Suppl 2:39–40.

Mazepa AW, Kidd LB, Young KM, Trepanier LA. Clinical presentation of 26 anaplasma phagocytophilum-seropositive dogs residing in an endemic area. J Am Anim Hosp Assoc. 2010;46(6):405–12.

Eberts MD. Vissotto de Paiva Diniz PP, Beall MJ, Stillman BA, Chandrashekar R, Breitschwerdt EB: Typical and atypical manifestations of Anaplasma phagocytophilum infection in dogs. J Am Anim Hosp Assoc. 2011;47(6):e86–94.

Maretzki CH, Fisher DJ, Greene CE. Granulocytic ehrlichiosis and meningitis in a dog. J Am Vet Med Assoc. 1994;205(11):1554–6.

Jaderlund KH, Bergstrom K, Egenvall A, Hedhammar A. Cerebrospinal fluid PCR and antibody concentrations against Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in dogs with neurological signs. J Vet Intern Med. 2009;23(3):669–72.

Barber RM, Li Q, Diniz PP, Porter BF, Breitschwerdt EB, Claiborne MK, et al. Evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for Ehrlichia, Anaplasma, spotted fever group Rickettsia, Bartonella, and Borrelia species in canine neurological diseases (109 cases). J Vet Intern Med. 2010;24(2):372–8.

Lazzerini K, Tipold A, Kornberg M, Silaghi C, Mietze A, Lübke-Becker A, et al. Meningitis and meningoencephalitis of unknown etiology in dogs – are canine vector borne diseases (CVBD) triggering the diseases? In: Proceedings of 26th Symposium ESVN-ECVN2013; Paris. J Vet Intern Med. 2014;28(3):968-–969.

Glaze MB, Gaunt SD. Uveitis associated with Ehrlichia platys infection in a dog. J Am Vet Med Assoc. 1986;189(8):916–7.

Harrus S, Aroch I, Lavy E, Bark H. Clinical manifestations of infectious canine cyclic thrombocytopenia. Vet Rec. 1997;141(10):247–50.

Sainz A, Amusategui I, Tesouro MA. Ehrlichia platys infection and disease in dogs in Spain. J Vet Diagn Invest. 1999;11(4):382–4.

Cohn LA. Ehrlichiosis and related infections. Vet Clin North Am Small Anim Pract. 2003;33(4):863–84.

Mylonakis ME, Koutinas AF, Billinis C, Leontides LS, Kontos V, Papadopoulos O, et al. Evaluation of cytology in the diagnosis of acute canine monocytic ehrlichiosis (Ehrlichia canis): a comparison between five methods. Vet Microbiol. 2003;91(2–3):197–204.

Mylonakis ME, Borjesson DL, Leontides L, Siarkou VI, Theodorou K, Koutinas AF. Cytologic patterns of lymphadenopathy in canine monocytic ehrlichiosis. Vet Clin Pathol. 2011;40(1):78–83.

Otranto D, Testini G, Dantas-Torres F, Latrofa MS, Diniz PP, de Caprariis D, et al. Diagnosis of canine vector-borne diseases in young dogs: a longitudinal study. J Clin Microbiol. 2010;48(9):3316–24.

De Tommasi AS, Baneth G, Breitschwerdt EB, Stanneck D, Dantas-Torres F, Otranto D, et al. Anaplasma platys in Bone Marrow Megakaryocytes of Young Dogs. J Clin Microbiol. 2014;52(6):2231–4.

Harrus S, Alleman AR, Bark H, Mahan SM, Waner T. Comparison of three enzyme-linked immunosorbant assays with the indirect immunofluorescent antibody test for the diagnosis of canine infection with Ehrlichia canis. Vet Microbiol. 2002;86(4):361–8.

Waner T, Harrus S, Jongejan F, Bark H, Keysary A, Cornelissen AW. Significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by Ehrlichia canis. Vet Parasitol. 2001;95(1):1–15.

Wen B, Rikihisa Y, Mott JM, Greene R, Kim HY, Zhi N, et al. Comparison of nested PCR with immunofluorescent-antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline. J Clin Microbiol. 1997;35(7):1852–5.

Maggi RG, Birkenheuer AJ, Hegarty BC, Bradley JM, Levy MG, Breitschwerdt EB. Comparison of serological and molecular panels for diagnosis of vector-borne diseases in dogs. Parasit Vectors. 2014;7:127.

Harrus S, Waner T, Aizenberg I, Bark H. Therapeutic effect of doxycycline in experimental subclinical canine monocytic ehrlichiosis: evaluation of a 6-week course. J Clin Microbiol. 1998;36(7):2140–2.

Baneth G, Harrus S, Ohnona FS, Schlesinger Y. Longitudinal quantification of Ehrlichia canis in experimental infection with comparison to natural infection. Vet Microbiol. 2009;136(3–4):321–5.

Breitschwerdt EB, Hegarty BC, Hancock SI. Doxycycline hyclate treatment of experimental canine ehrlichiosis followed by challenge inoculation with two Ehrlichia canis strains. Antimicrob Agents Chemother. 1998;42(2):362–8.

Sainz A, Tesouro MA, Amusategui I, Rodriguez F, Mazzucchelli F, Rodriguez M. Prospective comparative study of 3 treatment protocols using doxycycline or imidocarb dipropionate in dogs with naturally occurring ehrlichiosis. J Vet Intern Med. 2000;14(2):134–9.

French TW, Harvey JW. Serologic diagnosis of infectious cyclic thrombocytopenia in dogs using an indirect fluorescent antibody test. Am J Vet Res. 1983;44(12):2407–11.

Chandrashekar R, Mainville CA, Beall MJ, O’Connor T, Eberts MD, Alleman AR, et al. Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs. Am J Vet Res. 2010;71(12):1443–50.

Alleman AR, Wamsley HL, Abbott J, et al. Experimental Anaplasma phagocytophilum infection of dogs by intravenous inoculation of human and canine isolates and treatment with doxycycline. Vet Pathol. 2007;44:19.

Alleman A, Chandrashekar R, Beall M, Cyr K, Barbet A, Lundgren A, et al. Experimental inoculation of dogs with a human isolate (NY18) of Anaplasma phagocytophilum and demonstration of persistent infection following doxycycline therapy. J Vet Intern Med. 2006;20:763.

Harrus S, Kenny M, Miara L, Aizenberg I, Waner T, Shaw S. Comparison of simultaneous splenic sample PCR with blood sample PCR for diagnosis and treatment of experimental Ehrlichia canis infection. Antimicrob Agents Chemother. 2004;48(11):4488–90.

Sainz A. Ehrlichiosis/Anaplasmosis. In: Miró G, Solano-Gallego L, editors. Enfermedades Vectoriales del perro y del gato. 1st ed. Madrid: Ed. Achalantis; 2011. p. 203–26.

Gal A, Harrus S, Arcoh I, Lavy E, Aizenberg I, Mekuzas-Yisaschar Y, et al. Coinfection with multiple tick-borne and intestinal parasites in a 6-week-old dog. Can Vet J. 2007;48(6):619–22.

Beall MJ, Chandrashekar R, Eberts MD, Cyr KE, Diniz PP, Mainville C, et al. Serological and molecular prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, and Ehrlichia species in dogs from Minnesota. Vector Borne Zoonotic Dis. 2008;8(4):455–64.

Grab DJ, Nyarko E, Barat NC, Nikolskaia OV, Dumler JS. Anaplasma phagocytophilum-Borrelia burgdorferi coinfection enhances chemokine, cytokine, and matrix metalloprotease expression by human brain microvascular endothelial cells. Clin Vaccine Immunol. 2007;14(11):1420–4.

Foley JE, Brown RN, Gabriel MW, Henn J, Drazenovich N, Kasten R, et al. Spatial analysis of the exposure of dogs in rural north-coastal California to vectorborne pathogens. Vet Rec. 2007;161(19):653–7.

Cortese L, Pelagalli A, Piantedosi D, Cestaro A, Di Loria A, Lombardi P, et al. Effects of therapy on haemostasis in dogs infected with Leishmania infantum, Ehrlichia canis, or both combined. Vet Rec. 2009;164(14):433–4.

Mekuzas Y, Gradoni L, Oliva G, Foglia Manzillo V, Baneth G. Ehrlichia canis and Leishmania infantum co-infection: a 3-year longitudinal study in naturally exposed dogs. Clin Microbiol Infect. 2009;15 Suppl 2:30–1.

Matthewman LA, Kelly PJ, Brouqui P, Raoult D. Further evidence for the efficacy of imidocarb dipropionate in the treatment of Ehrlichia canis infection. J S Afr Vet Assoc. 1994;65(3):104–7.

Price JE, Dolan TT. A comparison of the efficacy of imidocarb dipropionate and tetracycline hydrochloride in the treatment of canine ehrlichiosis. Vet Rec. 1980;107(12):275–7.

Eddlestone SM, Neer TM, Gaunt SD, Corstvet R, Gill A, Hosgood G, et al. Failure of imidocarb dipropionate to clear experimentally induced Ehrlichia canis infection in dogs. J Vet Intern Med. 2006;20(4):840–4.

Kelly PJ, Matthewman LA, Brouqui P, Raoult D. Lack of susceptibility of Ehrlichia canis to imidocarb dipropionate in vitro. J S Afr Vet Assoc. 1998;69(2):55–6.

Schaefer JJ, Kahn J, Needham GR, Rikihisa Y, Ewing SA, Stich RW. Antibiotic clearance of Ehrlichia canis from dogs infected by intravenous inoculation of carrier blood. Ann N Y Acad Sci. 2008;1149:263–9.

Theodorou K, Mylonakis ME, Siarkou VI, Leontides L, Koutinas AF, Koutinas CK, et al. Efficacy of rifampicin in the treatment of experimental acute canine monocytic ehrlichiosis. J Antimicrob Chemother. 2013;68(7):1619–26.

Maurin M, Bakken JS, Dumler JS. Antibiotic susceptibilities of Anaplasma (Ehrlichia) phagocytophilum strains from various geographic areas in the United States. Antimicrob Agents Chemother. 2003;47(1):413–5.

Diniz PPVP, Breitschwerdt E. Canine granulocytotropic anaplasmosis (A. phagocytophilum infection). In: Greene CE, editor. Infectious diseases of the dog and cat. 4th ed. Philadelphia: Saunders; 2011. p. 244–54.

Chang WL, Su WL, Pan MJ. Two-step PCR in the evaluation of antibiotic treatment for Ehrlichia platys infection. J Vet Med Sci. 1997;59(9):849–51.

Aroch I, Harrus S. The use of recombinant human colony stimulating factor and recombinant human erythropoietin in the treatment of severe pancytopenia due to canine monocytic ehrlichiosis. Israel J Vet Med. 2001;56(2):65–9.

Perille AL, Matus RE. Canine ehrlichiosis in six dogs with persistently increased antibody titers. J Vet Intern Med. 1991;5(3):195–8.

Massa KL, Gilger BC, Miller TL, Davidson MG. Causes of uveitis in dogs: 102 cases (1989-2000). Vet Ophthalmol. 2002;5(2):93–8.

Heeb HL, Wilkerson MJ, Chun R, Ganta RR. Large granular lymphocytosis, lymphocyte subset inversion, thrombocytopenia, dysproteinemia, and positive Ehrlichia serology in a dog. J Am Anim Hosp Assoc. 2003;39(4):379–84.

van Heerden J. A retrospective study on 120 natural cases of canine ehrlichiosis. J S Afr Vet Assoc. 1982;53(1):17–22.

Shipov A, Klement E, Reuveni-Tager L, Waner T, Harrus S. Prognostic indicators for canine monocytic ehrlichiosis. Vet Parasitol. 2008;153(1–2):131–8.

Mylonakis ME, Ceron JJ, Leontides L, Siarkou VI, Martinez S, Tvarijonaviciute A, et al. Serum acute phase proteins as clinical phase indicators and outcome predictors in naturally occurring canine monocytic ehrlichiosis. J Vet Intern Med. 2011;25(4):811–7.

Carrade DD, Foley JE, Borjesson DL, Sykes JE. Canine granulocytic anaplasmosis: a review. J Vet Intern Med. 2009;23(6):1129–41.

Martin AR, Brown GK, Dunstan RH, Roberts TK. Anaplasma platys: an improved PCR for its detection in dogs. Exp Parasitol. 2005;109(3):176–80.

Horowitz HW, Aguero-Rosenfeld M, Dumler JS, McKenna DF, Hsieh TC, Wu J, et al. Reinfection with the agent of human granulocytic ehrlichiosis. Ann Intern Med. 1998;129(6):461–3.

Dantas-Torres F. The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): from taxonomy to control. Vet Parasitol. 2008;152(3–4):173–85.

Therapy Tables [ www.esccap.org ]

Pereira CP, Oliveira PR, Furquim KC, Bechara GH, Camargo-Mathias MI. Effects of fipronil (active ingredient of Frontline) on salivary gland cells of Rhipicephalus sanguineus females (Latreille, 1806) (Acari: Ixodidae). Vet Parasitol. 2009;166(1–2):124–30.

Rudoler N, Baneth G, Eyal O, van Straten M, Harrus S. Evaluation of an attenuated strain of Ehrlichia canis as a vaccine for canine monocytic ehrlichiosis. Vaccine. 2012;31(1):226–33.