Gryllus bimaculatus extract protects against palmitate-induced β-cell death by inhibiting ceramide synthesis

Applied Biological Chemistry - Tập 65 - Trang 1-10 - 2022
Jung-Soon Han1, Woo-Jae Park2, Ie Byung Park3, Min Hee Kim4
1Department of Human Ecology (Food Science and Nutrition), Korea University, Seoul, Republic of Korea
2Department of Biochemistry, Chung-Ang University College of Medicine, Seoul, Republic of Korea
3Department of Endocrinology and Metabolism, Gil Medical Center, College of Medicine, Gachon University, Incheon, Republic of Korea
4Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, Republic of Korea

Tóm tắt

Type I diabetes mellitus is an autoimmune disease characterized by the destruction of β-cells, leading to severe insulin deficiency. Environmental factors and genetic predisposition are implicated in β-cell destruction, which is the final step in a cascade of complex events. Possible triggers of β-cell destruction are activation of Fas, activation of perforin, increased generation of reactive oxygen species, increased production of inflammatory cytokines, and endoplasmic reticulum (ER) stress. In this study, we examined whether Gryllus bimaculatus (GB) extract could prevent palmitate-induced β-cell apoptosis. Exposure to GB extract prevented palmitate-induced death of MIN6 cells, a mouse pancreatic β-cell line. Palmitate increased total ceramide levels with the elevation of ceramide synthase (CerS)1, CerS4, and CerS6 expressions. Treatment with GB extract decreased the levels and expressions of ceramides related to insulin resistance. CerS4 and CerS6 overexpression, but not CerS1 overexpression, increased palmitate-induced MIN6 cell death by increasing ceramide synthesis. Oppositely, inhibition of ceramide synthesis by fumonisin B1 treatment partially recovered palmitate-induced MIN6 cell death. Furthermore, GB extract reduced ER stress (phosphorylation of PERK and eIF2α), NF-κB–iNOS signaling, and the phosphorylation of MAP kinase (JNK, p38). GB extract reduced pro-apoptotic Bax protein expression but increased anti-apoptotic Bcl2 expression. In addition, CerS4 and CerS6 overexpression aggravated impairment of insulin secretion by palmitate, but GB extract recovered it. In conclusion, GB could be a functional food that improves palmitate-induced β-cell death and insulin secretion.

Từ khóa

#Applied Microbiology #Bioorganic Chemistry #Biological Techniques

Tài liệu tham khảo

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