Growth characteristics of Glycyrrhiza glabra cell suspension cultures
Tóm tắt
Callus and cell suspension cultures of licorice (Glycyrrhiza glabra) have been established in Gamborg's B5 medium supplemented with 2% sucrose, 1.0 mg 1-1 2,4-dichlorophenoxyacetic acid and 0.1 mg 1-1 kinetin. Biomass accumulation was affected by the density and age of inoculum, vessel size and sucrose level. 8% sucrose medium in 2-1 flasks induced biphasic growth, with fructose as the only source of carbon and energy in the second phase.
Tài liệu tham khảo
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