Growth and Physiology of Maize (Zea mays L.) in a Nickel-Contaminated Soil and Phytoremediation Efficiency Using EDTA

Journal of Plant Growth Regulation - Tập 40 - Trang 774-786 - 2020
Muhammad Imran Tipu1, Muhammad Yasin Ashraf2, Nadeem Sarwar2, Muhammad Akhtar2, Muhammad Rashid Shaheen3, Sajjad Ali4, Christos A. Damalas5
1Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
2Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
3Department of Horticultural Sciences, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
4Department of Entomology, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
5Department of Agricultural Development, Democritus University of Thrace, Orestiada, Greece

Tóm tắt

Nickel (Ni) element is strongly phytotoxic at high concentrations for several plants, but due to its dual behavior and complicated chemistry, it has received little attention in plant nutrition and relevant experimental data are limited. The current research was carried out to study the effect of Ni on maize (Zea mays L.) growth and phytoextraction potential with EDTA assistance, a process termed as chemical assisted phytoremediation. Treatments included two levels of EDTA (0 and 0.5 mM), two levels of Ni (0 and 40 µM) and their combination (EDTA+Ni) that were applied to maize plants grown in a pot experiment. Application of Ni alone or in combination with EDTA reduced maize root and shoot length by 7.8% to 13.3% and by 15.6% to 21.1%, respectively, compared with control, as well as root and shoot dry weight by 42.0% to 60.0% and by 29.8% to 46.6%, respectively. A similar declining trend was observed also for the content of photosynthetic pigments (chl-a, chl-b, total chlorophyll, and carotenoids) as well as total proteins. However, proline, total soluble sugars, and free amino acids showed an increasing trend with application of Ni and EDTA alone or in combination. These treatments significantly decreased P and Na content in maize roots, stems, leaves, and grains, while increased K content compared with control. Application of EDTA with Ni was the most effective treatment to enhance Ni accumulation in maize (50.23 mg per plant) compared with Ni alone (40.62 mg per plant), EDTA alone (27.75 mg per plant), and control (15.51 mg per plant). Application of EDTA in combination with Ni enhanced Ni accumulation by 4.9 folds in maize shoots and by 2.6 folds in roots over control. In conclusion, application of EDTA in suitable concentrations may enhance Ni uptake by maize providing an effective and economic phytoremediation method of Ni-contaminated soils.

Tài liệu tham khảo

Ahammed GJ, Li X, Yang Y, Liu C, Zhou G, Wan H, Cheng Y (2020a) Tomato WRKY81 acts as a negative regulator for drought tolerance by modulating guard cell H2O2-mediated stomatal closure. Environ Exp Bot 171:103960 Ahammed GJ, Wang Y, Mao Q, Wu M, Yan Y, Ren J, Wang X, Liu A, Chen S (2020b) Dopamine alleviates bisphenol A-induced phytotoxicity by enhancing antioxidant and detoxification potential in cucumber. Environ Pollut 259:113957 Ahammed GJ, Wu M, Wang Y, Yan Y, Mao Q, Ren J, Ma R, Liu A, Chen S (2020c) Melatonin alleviates iron stress by improving iron homeostasis, antioxidant defense and secondary metabolism in cucumber. Sci Hortic 265:109205 Ain Q, Akhtar J, Amjad M, Haq M, Saqib Z (2016) Effect of enhanced nickel levels on wheat plant growth and physiology under salt stress. Commun Soil Sci Plant Anal 47:2538–2546 Ali H, Khan E, Sajad MA (2013) Phytoremediation of heavy metals: concepts and applications. Chemosphere 91:869–881 Amari T, Ghnaya T, Debez A, Taamali M, Youssef NB, Lucchini G (2014) Comparative Ni tolerance and accumulation potentials between Mesembryanthemum crystallinum (halophyte) and Brassica juncea: metal accumulation, nutrient status and photosynthetic activity. J Plant Physiol 171:1634–1644 Anamika S, Eapen S, Fulekar MH (2009) Phytoremediation of cadmium, lead and zinc by Brassica juncea L. Czern Coss J Appl Biosci 13:726–736 Baccouch S, Chaoui A, El Ferjani E (1998) Nickel toxicity: effects on growth and metabolism of maize. J Plant Nutr 21:577–588 Bates L, Waldren R, Teare I (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207 Ci D, Jiang D, Wollenweber B, Dai T, Jing Q, Cao W (2010) Genetic variance in cadmium tolerance and accumulation in wheat materials differing in ploidy and genome at seedling stage. J Agron Crop Sci 196:302–310 Dan TV, KrishnaRaj S, Saxena PK (2000) Metal tolerance of scented geranium (Pelargonium sp. ‘Frensham’): effects of cadmium and nickel on chlorophyll fluorescence kinetics. Int J Phytoremed 2:91–104 Davis P (1957) A method for the determination of chlorophyll in sea water. Marine CSIRO Aust. Div. Fish. Oceanogr. Rep. No. 7 Gajewska E, Skłodowska M (2009) Nickel-induced changes in nitrogen metabolism in wheat shoots. J Plant Physiol 166:1034–1044 Gajewska E, Skłodowska M, Słaba M, Mazur J (2006) Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots. Biol Plant 50:653–659 Ganie SA, Ahammed GJ, Wani SH (2020) Vascular plant one zinc-finger (VOZ) transcription factors: novel regulators of abiotic stress tolerance in rice (Oryza sativa L.). Gen Res Crop Evol 67:799–807 Garbisu C, Alkorta I (2001) Phytoextraction: a cost-effective plant-based technology for the removal of metals from the environment. Biores Technol 77:229–236 Ghasemi F, Heidari R, Jameii R, Purakbar L (2012) Effects of Ni2+ toxicity on Hill reaction and membrane functionality in maize. J Stress Physiol Biochem 8:55–61 Hasan MK, Ahammed GJ, Sun S, Li M, Yin H, Zhou J (2019) Melatonin inhibits cadmium translocation and enhances plant tolerance by regulating sulfur uptake and assimilation in Solanum lycopersicum L. J Agric Food Chem 67:10563–10576 He ZL, Yang XE, Stoffella PJ (2005) Trace elements in agroecosystems and impacts on the environment. J Trace Elem Medic Biol 19:125–140 Kaur L, Gadgil K, Sharma S (2015) Comparative evaluation of salicylic acid and EDTA chelant induced phytoremediation of lead and nickel using Lemna minor L. Trop Plant Res 2:264–270 Kevrešan S, Petrović N, Popović M, Kandrač J (1998) Effect of heavy metals on nitrate and protein metabolism in sugar beet. Biol Plant 41:235–240 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275 Maxted A, Black C, West H, Crout N, McGrath S, Young S (2007) Phytoextraction of cadmium and zinc from arable soils amended with sewage sludge using Thlaspi caerulescens: development of a predictive model. Environ Pollut 150:363–372 Negi A, Singh HP, Batish DR, Kohli RK (2014) Ni+2-inhibited radicle growth in germinating wheat seeds involves alterations in sugar metabolism. Acta Physiol Plant 36:923–929 Ouzounidou G, Moustakas M, Symeonidis L, Karataglis S (2006) Response of wheat seedlings to Ni stress: effects of supplemental calcium. Arch Environ Contam Toxic 50:346–352 Page A (1982) Methods of soil analysis: chemical and microbiological properties. American Society of Agronomy, Madison, WI, USA Pandey N, Sharma CP (2002) Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Plant Sci 163:753–758 Ramarao C, Patil V, Dhak B, Kadrekar S (1983) A simple in vivo method for the determination of nitrite reductase activity in rice roots. Z Pflanzenphysiol 109:81–85 Rew A (2007) Phytoremediation: an environmentally sound technology for pollution prevention, control and remediation in developing countries. Educ Res Rev 2:151–156 Riazi A, Matsuda K, Arslan A (1985) Water-stress induced changes in concentrations of proline and other solutes in growing regions of young barley leaves. J Exp Bot 36:1716–1725 Rinklebe J, Shaheen SM (2017) Redox chemistry of nickel in soils and sediments: a review. Chemosphere 179:265–278 Rus A, Estan M, Gisbert C, Garcia-Sogo B, Serrano R, Caro M (2001) Expressing the yeast HAL1 gene in tomato increases fruit yield and enhances K+/Na+ selectivity under salt stress. Plant Cell Environ 24:875–880 Salt DE, Blaylock M, Kumar NP, Dushenkov V, Ensley BD, Chet I (1995) Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants. Nat Biotechnol 13:468–474 Sarwar N, Imran M, Shaheen MR, Ishaq W, Kamran A, Matloob A (2017) Phytoremediation strategies for soils contaminated with heavy metals: modifications and future perspectives. Chemosphere 171:710–721 Seregin I, Ivanov V (2001) Physiological aspects of cadmium and lead toxic effects on higher plants. Russ J Plant Physiol 48:523–544 Seth CS (2012) A review on mechanisms of plant tolerance and role of transgenic plants in environmental clean-up. Bot Rev 78:32–62 Shabani N, Sayadi M (2012) Evaluation of heavy metals accumulation by two emergent macrophytes from the polluted soil: an experimental study. Environmentalist 32:91–98 Shafeeq A, Butt ZA, Muhammad S (2012) Response of nickel pollution on physiological and biochemical attributes of wheat (Triticum aestivum L.) var. Bhakar-02. Pak J Bot 44:111–116 Sheoran I, Singal H, Singh R (1990) Effect of cadmium and nickel on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeonpea (Cajanus cajan L.). Photosynth Res 23:345–351 Sun Y, Zhou Q, Xu Y, Wang L, Liang X (2011) The role of EDTA on cadmium phytoextraction in a cadmium-hyperaccumulator Rorippa globosa. J Environ Chem Ecotox 3:45–51 Sym GJ (1984) Optimisation of the in-vivo assay conditions for nitrate reductase in barley (Hordeum vulgare L. cv. Igri). J Sci Food Agric 35:725–730 Tian Y, Zhang H, Guo W, Chen Z, Wei X, Zhang L, Han L, Dai L (2012) Assessment of the phytoremediation potential of bioenergy crop maize (Zea mays) in soil contaminated by cadmium: Morphology, photosynthesis and accumulation. Fresen Environ Bull 21:3575–3581 Tong Y-P, Kneer R, Zhu Y-G (2004) Vacuolar compartmentalization: a second-generation approach to engineering plants for phytoremediation. Trends Plant Sci 9:7–9 Turgut C, Pepe MK, Cutright TJ (2004) The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus. Environ Pollut 131:147–154 Van Slyke DD, Dillon RT, MacFadyen DA, Hamilton P (1941) Gasometric determination of carboxyl groups in free amino acids. J Biol Chem 141:627–669 Velikova V, Tsonev T, Loreto F, Centritto M (2011) Changes in photosynthesis, mesophyll conductance to CO2, and isoprenoid emissions in Populus nigra plants exposed to excess nickel. Environ Pollut 159:1058–1066 Yusuf M, Fariduddin Q, Hayat S, Ahmad A (2011) Nickel: an overview of uptake, essentiality and toxicity in plants. Bull Environ Contam Toxic 86:1–17 Zhang Y, Liang Y, Zhao X, Jin X, Hou L, Shi Y, Ahammed GJ (2019) Silicon compensates phosphorus deficit-induced growth inhibition by improving photosynthetic capacity, antioxidant potential, and nutrient homeostasis in tomato. Agronomy 9:733 Zhang Z, Wu P, Zhang W, Yang Z, Liu H, Ahammed GJ, Cui J (2020) Calcium is involved in exogenous NO-induced enhancement of photosynthesis in cucumber (Cucumis sativus L.) seedlings under low temperature. Sci Hortic 261:108953 Zhou Y, Guang Y, Li J, Wang F, Ahammed GJ, Yang Y (2019) The CYP74 gene family in watermelon: genome-wide identification and expression profiling under hormonal stress and root-knot nematode infection. Agronomy 9:872