Green synthesis and characterization of selenium nanoparticles (Se NPs) from the skin (testa) of Pistacia vera L. (Siirt pistachio) and investigation of antimicrobial and anticancer potentials

Mehmet Fırat Baran1,2, Cumali Keskin1, Ayşe Baran3, Kadri Kurt2, Polat İpek4, Aziz Eftekhari5, Rovshan Khalilov6, Ismayil Fridunbayov6, William C. Cho7
1Department of Medical Services and Techniques, Vocational School of Health Services, Mardin Artuklu University, Mardin, Turkey
2Department of Food Technology, Vocational School of Technical Sciences, Batman University, Batman, Turkey
3Department of Biology, Mardin Artuklu University Graduate Education Institute, Mardin, Turkey
4Department of Physiology, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, Turkey
5Department of Biochemistry, Faculty of Science, Ege University, İzmir, Turkey
6Department of Biophysics and Biochemistry, Baku State University, Baku, Azerbaijan
7Department of Clinical Oncology, Queen Elizabeth Hospital, Kowloon, China

Tóm tắt

Metallic nanoparticles created by ecologically friendly synthesis processes are becoming increasingly useful in a variety of applications. Because of their strong bioactive component qualities, biocompatible architectures, high stability, and low toxicity, green-produced selenium nanoparticles are particularly significant materials for various medicinal applications. Plants include a wealth of essential phytochemicals with therapeutic and medical capabilities. Pistachio vera L. (Siirt pistachio) is a seasonal fruit that is frequently consumed for its nutritional worth and health advantages. However, the outer colored skin of the P. vera (Siirt pistachio) fruit, which is not consumed, contains many biologically active compounds. In this study, plant-mediated synthesis of selenium nanoparticles (SeNPs) was successfully accomplished after adding the sodium selenite solution to the aqueous extract of P. vera colored skin waste. The synthesized Se NPs were characterized with UV–Vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta distribution, atomic force microscope (AFM), Fourier transform infrared spectrum (FTIR), thermogravimetric (TGA) and differential thermal analysis, X-ray diffraction (XRD), and energy-dispersive X-ray spectrum (EDX). Biogenic Pistacia vera (PV) PV-SeNPs were also tested for their ability to suppress the development of various pathogenic bacteria and cancerous cell lines. The UV-visible (UV–Vis) spectra revealed an absorption peak at 325 nm, which reflected the surface plasmon band. The significant selenium signal on the EDX spectrum at 1.5 keV confirmed the creation of Se NPs. The presence of several peaks on the FTIR spectrum of the aqueous extract of Pv and the nanoparticles indicated the presence of some important functional groups such as amines, carbonyl compounds, and phenols, which are important in facilitating the process of capping and bioreduction, as well as conferring stability to nanoparticles. The TEM microphotographs revealed that the nanoparticles were highly distributed, had a spherical morphological form, and were monodisperse below 10 nm. Biogenic Pv-SeNPs exhibited similar antimicrobial activity as standard antibiotics. However, it was determined that the cytotoxic activity of Se-NPs against cancer cell lines was quite high depending on the dose and time. As a result, the Pv-SeNPs are likely to be extremely beneficial in the pharmaceutical and cosmetic sectors, as well as in the food and cosmetic industries, in producing antimicrobial and/or anticancer medications. Graphical Abstract Of Green Synthesis, Characterization, And Biomedical Application of Pv-SeNPs

Tài liệu tham khảo

Khalilov R (2023) A comprehensive review of advanced nano-biomaterials in regenerative medicine and drug delivery. Adv Biol Earth Sci 8(1):5–18 Ikram M, Javed B, Raja NI, Mashwani ZUR (2021) Biomedical potential of plant-based selenium nanoparticles: a comprehensive review on therapeutic and mechanistic aspects. Int J Nanomed 16:249–268 Gunashova GY (2022) Synthesis of silver nanoparticles using a thermophilic bacterium strain isolated from the spring Yukhari Istisu of the Kalbajar region (AZERBAIJAN). Adv Biol Earth Sci 7(3):198–204 Ramazanli VN, Ahmadov IS (2022) Synthesis of silver nanoparticles by using extract of olive leaves. Adv Biol Earth Sci 7(3):238–244 He H, Liu C, Shao C, Wu Y, Huang Q (2022) Green synthesis of ultrasmall selenium nanoparticles (SeNPs) using Hericium erinaceus polysaccharide (HEP) as nanozymes for efficient intracellular antioxidation. Mater Lett 317:132079 Hatipoğlu A, Baran A, Keskin C, Baran MF, Eftekhari A, Omarova S, Kandemir Sİ (2023) Green synthesis of silver nanoparticles based on the Raphanus sativus leaf aqueous extract and their toxicological/microbiological activities. Environ Sci Pollut Res 1–13. https://doi.org/10.1007/s11356-023-26499-z Baran MF, Keskin C, Baran A, Hatipoğlu A, Yildiztekin M, Küçükaydin S, Eftekhari A (2023) Green synthesis of silver nanoparticles from Allium cepa L. peel extract, their antioxidant, antipathogenic, and anticholinesterase activity. Molecules 28(5):2310 Chen X, Xue Z, Ji J, Wang D, Shi G, Zhao L, Feng S (2021) Hedysarum polysaccharides mediated green synthesis of gold nanoparticles and study of its characteristic, analytical merit, catalytic activity. Mater Res Bull 133:111070 Alipour S, Kalari S, Morowvat MH, Sabahi Z, Dehshahri A (2021) Green synthesis of selenium nanoparticles by cyanobacterium Spirulina platensis (abdf2224): cultivation condition quality controls. Biomed Res Int 2021:1–11 Vu TT, Nguyen PTM, Pham NH, Le TH, Nguyen TH, Do DT, La DD (2022) Green synthesis of selenium nanoparticles using Cleistocalyx operculatus leaf extract and their acute oral toxicity study. J Compos Sci 6(10):307 Nikam PB, Salunkhe JD, Minkina T, Rajput VD, Kim BS, Patil SV (2022) A review on green synthesis and recent applications of red nano Selenium. Results Chem 4:100581 Chen N, Yao P, Zhang W, Zhang Y, Xin N, Wei H, Zhao C (2022) Selenium nanoparticles: enhanced nutrition and beyond. Crit Rev Food Sci Nutr 2022:1–12 Alagesan V, Venugopal S (2019) Green synthesis of selenium nanoparticle using leaves extract of Withania somnifera and its biological applications and photocatalytic activities. Bionanoscience 9:105–116 Ezhuthupurakkal PB, Polaki LR, Suyavaran A, Subastri A, Sujatha V, Thirunavukkarasu C (2017) Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of calf thymus DNA through intercalation and groove binding. Mater Sci Eng, C 74:597–608 Cittrarasu V, Kaliannan D, Dharman K, Maluventhen V, Easwaran M, Liu WC, Arumugam M (2021) Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities. Sci Rep 11(1):1032 Tomaino A, Martorana M, Arcoraci T, Monteleone D, Giovinazzo C, Saija A (2010) Antioxidant activity and phenolic profile of pistachio (Pistacia vera L., variety Bronte) seeds and skins. Biochimie 92(9):1115–1122 Wang LS, Stoner GD (2008) Anthocyanins and their role in cancer prevention. Cancer Lett 269(2):281–290 Fadl AM, El-Kholy EMS, Abulyazid I, Shoman AA, Awad HH, Mohammed HS (2022) Radiation-assisted green synthesis and characterization of selenium nanoparticles, and larvicidal effects on Culex pipiens complex. J Cluster Sci 33(6):2601–2615 Puri A, Patil S (2022) Tinospora cordifolia stem extract-mediated green synthesis of selenium nanoparticles and its biological applications. Pharmacogn Res 14(3):1–7 Vundela SR, Kalagatur NK, Nagaraj A, Kadirvelu K, Chandranayaka S, Kondapalli K, Poda S (2022) Multi-biofunctional properties of phytofabricated selenium nanoparticles from Carica papaya fruit extract: antioxidant, antimicrobial, antimycotoxin, anticancer, and biocompatibility. Front Microbiol 12:4374 Ramamurthy CH, Sampath KS, Arunkumar P, Kumar MS, Sujatha V, Premkumar K, Thirunavukkarasu C (2013) Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst Eng 36:1131–1139 Zeraatkar S, Tahan M, Sadeghian H, Nazari R, Behmadi M, Hosseini Bafghi M (2023) Effect of biosynthesized selenium nanoparticles using Nepeta extract against multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii. J Basic Microbiol 63(2):210–222 Shirmehenji R, Javanshir S, Honarmand M (2021) A green approach to the bio-based synthesis of selenium nanoparticles from mining waste. J Cluster Sci 32:1311–1323 Fouda A, Al-Otaibi WA, Saber T, AlMotwaa SM, Alshallash KS, Elhady M, Abdel-Rahman MA (2022) Antimicrobial, antiviral, and in-vitro cytotoxicity and mosquitocidal activities of Portulaca oleracea-based green synthesis of selenium nanoparticles. J Funct Biomater 13(3):157 Rosaiah G, Mangamuri UK, Sikharam AS, Devaraj K, Kalagatur NK, Kadirvelu K (2022) Biosynthesis of selenium nanoparticles from Annona muricata fruit aqueous extract and investigation of their antioxidant and antimicrobial potentials. Curr Trends Biotechnol Pharm 16(1):101–107 Hashem AH, Selim TA, Alruhaili MH, Selim S, Alkhalifah DHM, Al Jaouni SK, Salem SS (2022) Unveiling antimicrobial and insecticidal activities of biosynthesized selenium nanoparticles using prickly pear peel waste. J Funct Biomater 13(3):112 Saranya T, Ramya S, Kavithaa K, Paulpandi M, Cheon YP, Harysh Winster S, Narayanasamy A (2022) Green synthesis of selenium nanoparticles using Solanum nigrum fruit extract and its anti-cancer efficacy against triple negative breast cancer. J Clust Sci 1–11 Sheikhlou K, Allahyari S, Sabouri S, Najian Y, Jafarizadeh-Malmiri H (2020) Walnut leaf extract-based green synthesis of selenium nanoparticles via microwave irradiation and their characteristics assessment. Open Agric 5(1):227–235 Kazemi M, Akbari A, Sabouri Z, Soleimanpour S, Zarrinfar H, Khatami M, Darroudi M (2021) Green synthesis of colloidal selenium nanoparticles in starch solutions and investigation of their photocatalytic, antimicrobial, and cytotoxicity effects. Bioprocess Biosyst Eng 44:1215–1225 Ashengroph M, Tozandehjani S (2022) Optimized resting cell method for green synthesis of selenium nanoparticles from a new Rhodotorula mucilaginosa strain. Process Biochem 116:197–205 Salem SS (2022) Bio-fabrication of selenium nanoparticles using Baker’s yeast extract and its antimicrobial efficacy on food borne pathogens. Appl Biochem Biotechnol 194(5):1898–1910 Younas M, Rasool MH, Khurshid M, Khan A, Nawaz MZ, Ahmad I, Lakhan MN (2023) Moringa oleifera leaf extract mediated green synthesis of silver nanoparticles and their antibacterial effect against selected gram-negative strains. Biochem Syst Ecol 107:104605 Alizadeh SR, Seyedabadi M, Montazeri M, Khan BA, Ebrahimzadeh MA (2023) Allium paradoxum extract mediated green synthesis of SeNPs: assessment of their anticancer, antioxidant, iron chelating activities, and antimicrobial activities against fungi, ATCC bacterial strains, Leishmania parasite, and catalytic reduction of methylene blue. Mater Chem Phys 296:127240 Hatami R, Javadi A, Jafarizadeh-Malmiri H (2020) Effectiveness of six different methods in green synthesis of selenium nanoparticles using propolis extract: Screening and characterization. Green Process Synth 9(1):685–692 Gharbavi M, Mousavi M, Pour‐Karim M, Tavakolizadeh M, Sharafi A (2022) Biogenic and facile synthesis of selenium nanoparticles using Vaccinium arctostaphylos L. fruit extract and anticancer activity against in vitro model of breast cancer. Cell Biol Int 46(10):1612–1624 Meenambigai K, Kokila R, Chandhirasekar K, Thendralmanikandan A, Kaliannan D, Ibrahim KS, Nareshkumar A (2022) Green synthesis of selenium nanoparticles mediated by Nilgirianthus ciliates leaf extracts for antimicrobial activity on foodborne pathogenic microbes and pesticidal activity against Aedes aegypti with molecular docking. Biol Trace Elem Res 200(6):2948–2962 Wadhwani SA, Gorain M, Banerjee P, Shedbalkar UU, Singh R, Kundu GC, Chopade BA (2017) Green synthesis of selenium nanoparticles using Acinetobacter sp. SW30: optimization, characterization and its anticancer activity in breast cancer cells. Int J Nanomed 12:6841 Ahmed KBA, Raman T, Veerappan A (2016) Future prospects of antibacterial metal nanoparticles as enzyme inhibitor. Mater Sci Eng C 68:939–947 Babu B, Palanisamy S, Vinosha M, Anjali R, Kumar P, Pandi B, Prabhu NM (2020) Bioengineered gold nanoparticles from marine seaweed Acanthophora spicifera for pharmaceutical uses: antioxidant, antibacterial, and anticancer activities. Bioprocess Biosyst Eng 43:2231–2242 Maillard APF, Dalmasso PR, de Mishima BAL, Hollmann A (2018) Interaction of green silver nanoparticles with model membranes: possible role in the antibacterial activity. Colloids Surf B 171:320–326 Donga S, Bhadu GR, Chanda S (2020) Antimicrobial, antioxidant and anticancer activities of gold nanoparticles green synthesized using Mangifera indica seed aqueous extract. Artif Cells Nanomed Biotechnol 48(1):1315–1325 Cui Y, Zhao Y, Tian Y, Zhang W, Lü X, Jiang X (2012) The molecular mechanism of action of bactericidal gold nanoparticles on Escherichia coli. Biomaterials 33(7):2327–2333 Jha P, Saraf A, Rath D, Sharma D (2017) Green synthesis and antimicrobial property of gold nanoparticles: a review. World J Pharmaceutıcal Med Res 3(8):431–435 Srivastava N, Mukhopadhyay M (2015) Green synthesis and structural characterization of selenium nanoparticles and assessment of their antimicrobial property. Bioprocess Biosyst Eng 38:1723–1730 Perumal S, Gopal Samy MV, Subramanian D (2021) Selenium nanoparticle synthesis from endangered medicinal herb (Enicostema axillare). Bioprocess Biosyst Eng 44:1853–1863 Remya RR, Rajasree SR, Aranganathan L, Suman TY (2015) An investigation on cytotoxic effect of bioactive AgNPs synthesized using Cassia fistula flower extract on breast cancer cell MCF-7. Biotechnol Reports 8:110–115 Lee KX, Shameli K, Yew YP, Teow SY, Jahangirian H, Rafiee-Moghaddam R, Webster TJ (2020) Recent developments in the facile bio-synthesis of gold nanoparticles (AuNPs) and their biomedical applications. Int J Nanomed 275–300 Doan VD, Thieu AT, Nguyen TD, Nguyen VC, Cao XT, Nguyen TLH, Le VT (2020) Biosynthesis of gold nanoparticles using Litsea cubeba fruit extract for catalytic reduction of 4-nitrophenol. J Nanomater 2020:4548790 Mehravani B, Ribeiro AI, Zille A (2021) Gold nanoparticles synthesis and antimicrobial effect on fibrous materials. Nanomaterials 11(5):1067 Hosny M, Fawzy M, Abdelfatah AM, Fawzy EE, Eltaweil AS (2021) Comparative study on the potentialities of two halophytic species in the green synthesis of gold nanoparticles and their anticancer, antioxidant and catalytic efficiencies. Adv Powder Technol 32(9):3220–3233 Chen J, Li Y, Fang G, Cao Z, Shang Y, Alfarraj S, ... Duan X (2021) Green synthesis, characterization, cytotoxicity, antioxidant, and anti-human ovarian cancer activities of Curcumae kwangsiensis leaf aqueous extract green-synthesized gold nanoparticles. Arabian J Chem 14(3):103000 Rolim WR, Pelegrino MT, de Araújo Lima B, Ferraz LS, Costa FN, Bernardes JS, Seabra AB (2019) Green tea extract mediated biogenic synthesis of silver nanoparticles: characterization, cytotoxicity evaluation and antibacterial activity. Appl Surf Sci 463:66–74 Barabadi H, Webster TJ, Vahidi H, Sabori H, Kamali KD, Shoushtari FJ, ... Saravana M (2020) Green nanotechnology-based gold nanomaterials for hepatic cancer therapeutics: a systematic review. Iranian J Pharm Res: IJPR 19(3):3–17 Hosny M, Fawzy M, El-Badry YA, Hussein EE, Eltaweil AS (2022) Plant-assisted synthesis of gold nanoparticles for photocatalytic, anticancer, and antioxidant applications. J Saudi Chem Soc 26(2):101419 Tuyen NNK, Huong QTT, Nam NTH, Hai ND, Tinh NT, Buu TT, ... Hieu NH (2023) Applicable orientation of eco-friendly phyto-synthesized selenium nanoparticles: bioactive investigation and dye photodegradation. Biomass Convers Biorefinery 1–17. https://doi.org/10.1007/s13399-023-03823-8 Hashem AH, Saied E, Ali OM et al (2023) Pomegranate peel extract stabilized selenium nanoparticles synthesis: promising antimicrobial potential, antioxidant activity, biocompatibility, and hemocompatibility. Appl Biochem Biotechnol. https://doi.org/10.1007/s12010-023-04326-y