Global ITS diversity in the Sporothrix schenckii complex
Tóm tắt
Molecular phylogeny has revealed that sporotrichosis is caused by several Sporothrix species which differ in clinical behavior. The complex is embedded within Ophiostoma, a genus mainly comprising fungi that live in association with bark beetles, but differs by a high virulence towards humans and other mammals. The different ecology is corroborated by phylogenetic separation. The aim of the present study was to determine the validity of the rDNA internal transcribed spacer (ITS) region as a marker for diagnostics of species in the clinical group, using beta-tubulin sequences to calibrate species delimitations. The topology of the two gene trees was concordant, and all clinically relevant Sporothrix species could easily be recognized by means of the ITS region. An increased geographic sampling did not affected delimitation success in the clinical clade of the S. schenckii complex.
Tài liệu tham khảo
Aghayeva DN, Wingfield MJ, de Beer ZW, Kirisits T (2004) Two new Ophiostoma species with Sporothrix anamorphs from Austria and Azerbaijan. Mycologia 96:866–878
Aghayeva DN, Wingfield MJ, Kirisits T, Wingfield BD (2005) Ophiostoma dentifundum sp. nov. from oak in Europe, characterized using molecular phylogenetic data and morphology. Mycol Res 109:1127–1136. doi:10.1017/S0953756205003710
Arrillaga-Moncrieff I, Capilla J, Mayayo E, Marimon R, Mariné M, Gené J, Cano J, Guarro J (2009) Different virulence levels of the species of Sporothrix in a murine model. Clin Microbiol Infect 15:651–655. doi:10.1111/j.1469-0691.2009.02824.x
Barros MB, Schubach TP, Coll JO, Gremião ID, Wanke B, Schubach A (2010) Sporotrichosis: development and challenges of an epidemic. Rev Panam Salud Publica 27:455–460. doi:10.1590/S1020-49892010000600007
Berbee ML, Taylor JW (1992) 18S Ribosomal RNA gene sequence characters place the human pathogen Sporothrix schenckii in the genus Ophiostoma. Exp Mycol 16:87–91. doi:10.1016/0147-5975(92)90044-R
Bommer M, Hütter ML, Stilgenbauer S, de Hoog GS, de Beer ZW, Wellinghausen N (2009) Fatal Ophiostoma piceae infection in a patient with acute lymphoblastic leukaemia. J Med Microbiol 58:381–385. doi:10.1099/jmm.0.005280-0
Callens SF, Kitetele F, Lukun P, Lelo P, van Rie A, Behets F, Colebunders R (2006) Pulmonary Sporothrix schenckii infection in a HIV positive child. J Trop Pediatr 52:144–146. doi:10.1093/tropej/fmi101
Criseo G, Romeo O (2010) Ribosomal DNA sequencing and phylogenetic analysis of environmental Sporothrix schenckii strains: comparison with clinical isolates. Mycopathologia 169:351–358. doi:10.1007/s11046-010-9274-9
de Beer ZW, Harrington TC, Vismer HF, Wingfield BD, Wingfield MJ (2003) Phylogeny of the Ophiostoma stenoceras-Sporothrix schenckii complex. Mycologia 95(3):434–41
de Hoog GS (1974) The genera Blastobotrys, Sporothrix, Calcarisporium and Calcarisporiella gen. nov. Stud Mycol 7:1–119
de Hoog GS, Guarro J, Gené J, Figueras MJ (2000) Atlas of clinical fungi, 2nd edn. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands
de Meyer EM, de Beer ZW, Summerbell RC, Moharram AM, de Hoog GS, Vismer HF, Wingfield MJ (2008) Taxonomy and phylogeny of new wood- and soil-inhabiting Sporothrix species in the Ophiostoma stenoceras-Sporothrix schenckii complex. Mycologia 100:647–661. doi:10.3852/07-157R
Dixon DM, Salkin IF, Duncan RA, Hurd NJ, Haines JH, Kemna ME, Coles FB (1991) Isolation and characterization of Sporothrix schenckii from clinical and environmental sources associated with the largest U.S. epidemic of sporotrichosis. J Clin Microbiol 29:1106–1113
Feeney KT, Arthur IH, Whittle AJ, Altman SA, Speers DJ (2007) Outbreak of sporotrichosis, Western Australia. Emerg Infect Dis 13:1228–1231. doi:10.3201/eid1308.061462
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Fernandes GF, dos Santos PO, Rodrigues AM, Sasaki AA, Burger E, de Camargo ZP (2013) Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species. Virulence 4(3):1–9. doi:10.4161/viru.23112
Fernández-Silva F, Capilla J, Mayayo E, Guarro J (2012) Virulence of Sporothrix luriei in a murine model of disseminated infection. Mycopathologia 173:245–249. doi:10.1007/s11046-011-9506-7
Galhardo MC, De Oliveira RM, Valle AC, Paes Rde A, Silvatavares PM, Monzon A, Mellado E, Rodriguez-Tudela JL, Cuenca-Estrella M (2008) Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil. Med Mycol 46:141–151. doi:10.1080/13693780701742399
Guarro J, Gené J, Stchigel AM (1999) Developments in fungal taxonomy. Clin Microbiol Rev 12:454–500
Han SQ, Pei LR, Liu YX, Bao JH, Jiang B (2006) Sporotrichosis: clinical analysis of 43 cases. Chin J Mycol 1:267–269 (in Chinese)
Harrington TC, McNew D, Steimel J, Hofstra D, Farrell R (2001) Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi. Mycologia 93:111–136
Heinrichs G, de Hoog GS, Haase G (2012) Barcode identifiers as a practical tool for reliable species assignment of medically important black yeast species. J Clin Microbiol 50:3023–3030. doi:10.1128/JCM.00574-12
Howard DH (1961) Dimorphism of Sporotrichum schenckii. J Bacteriol 81:464–469
Jacobs K, Seifert KA, Harrison KJ, Kirisits T (2003) Identity and phylogenetic relationships of ophiostomatoid fungi associated with invasive and native Tetropium species (Coleoptera: Cerambycidae) in Atlantic Canada. Can J Bot 81:316–329. doi:10.1139/b03-025
Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066. doi:10.1093/nar/gkf436
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Li LL, Su HH, Liu JF et al (2007) DNA typing of Sporothrix schenkii from different areas. Chin J Leprosy Skin Dis 23:1043–1045 (in Chinese)
Madrid H, Cano J, Gené J, Bonifax A, Toriello C, Guarro J (2009) Sporothrix globosa, a pathogenic fungus with widespread geographical distribution. Rev Iberoam Micol 26:218–222
Madrid H, Gené J, Cano J, Silvera C, Guarro J (2010) Sporothrix brunneoviolacea and Sporothrix dimorphospora, two new members of the Ophiostoma stenoceras-Sporothrix schenckii complex. Mycologia 102:1193–1203. doi:10.3852/09-320
Mariat F (1971) Adaptation of Ceratocystis to a parasitic life in animals—acquisition of a pathogenicity comparable to Sporothrix schenckii. Sabouraudia 9:191–205
Marimon R, Gené J, Cano J, Trilles L, Dos Santos LM, Guarro J (2006) Molecular phylogeny of Sporothrix schenckii. J Clin Microbiol 44:3251–3256. doi:10.1128/JCM.00081-06
Marimon R, Cano J, Gené J, Sutton DA, Kawasaki M, Guarro J (2007) Sporothrix brasiliensis, S. globosa, and S. mexicana, three new Sporothrix species of clinical interest. J Clin Microbiol 45:3198–3206. doi:10.1128/JCM.00808-07
Marimon R, Gené J, Cano J, Guarro J (2008) Sporothrix luriei: a rare fungus from clinical origin. Med Mycol 46:621–625. doi:10.1080/13693780801992837
Massoumi Alamouti S, Tsui CK, Breuil C (2009) Multigene phylogeny of filamentous ambrosia fungi associated with ambrosia and bark beetles. Mycol Res 113:822–835. doi:10.1016/j.mycres.2009.03.003
Mei XL, Xia JX, Wang JY, Li X, Zhu WJ, Li FQ (2011) Clinical and pathological analysis on 100 cases of sporotrichosis. Chin J Mycol 6:203–206 (in Chinese)
Mesa-Arango AC, Del Rocío Reyes-Montes M, Pérez-Mejía A, Navarro-Barranco H, Souza V, Zúñiga G, Toriello C (2002) Phenotyping and genotyping of Sporothrix schenckii isolates according to geographic origin and clinical form of Sporotrichosis. J Clin Microbiol 40:3004–3011. doi:10.1128/JCM.40.8.3004-3011.2002
Oliveira MME, Almeida-Paes R, Muniz MM, Gutierrez-Galhardo MC, Zancope-Oliveira RM (2011) Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of Sporotrichosis in Brazil. Mycopathologia 172:257–267. doi:10.1007/s11046-011-9437-3
O'Reilly LC, Altman SA (2006) Macrorestriction analysis of clinical and environmental isolates of Sporothrix schenckii. J Clin Microbiol 44:2547–2552. doi:10.1128/JCM.00078-06
Rodrigues A, Mueller UG, Ishak HD, Bacci M Jr, Pagnocca FC (2011) Ecology of microfungal communities in gardens of fungus-growing ants (Hymenoptera: Formicidae): a year-long survey of three species of attine ants in Central Texas. FEMS Microbiol Ecol 78:244–255. doi:10.1111/j.1574-6941.2011.01152.x
Rodrigues AM, de Hoog GS, Camargo ZP (2012) Emergence of pathogenicity in the Sporothrix schenckii complex. Med Mycol (in press). doi: 10.3109/13693786.2012.719648
Roets F, de Beer ZW, Dreyer LL, Zipfel R, Crous PW, Wingfield MJ (2006) Multi-gene phylogeny for Ophiostoma spp. reveals two new species from Protea infructescences. Stud Mycol 55:199–212. doi:10.3114/sim.55.1.199
Roets F, de Beer ZW, Wingfield MJ, Crous PW, Dreyer LL (2008) Ophiostoma gemellus and Sporothrix variecibatus from mites infesting Protea infructescences in South Africa. Mycologia 100:496–510. doi:10.3852/07-181R
Roets F, Wingfield BD, de Beer ZW, Wingfield MJ, Dreyer LL (2010) Two new Ophiostoma species from Protea caffra in Zambia. Persoonia 24:18–28. doi:10.3767/003158510X490392
Romeo O, Scordino F, Criseo G (2011) New insight into molecular phylogeny and epidemiology of Sporothrix schenckii species complex based on calmodulin-encoding gene analysis of Italian isolates. Mycopathologia 172:179–186. doi:10.1007/s11046-011-9420-z
Rosa AC, Scroferneker ML, Vettorato R, Gervini RL, Vettorato G, Weber A (2005) Epidemiology of sporotrichosis: a study of 304 cases in Brazil. J Am Acad Dermatol 52:451–459. doi:10.1016/j.jaad.2004.11.046
Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W et al (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA 109:6241–6246. doi:10.1073/pnas.1117018109
Schubach TM, Schubach A, Okamoto T, Barros MB, Figueiredo FB, Cuzzi T, Fialho-Monteiro PC, Reis RS, Perez MA, Wanke B (2004) Evaluation of an epidemic of sporotrichosis in cats: 347 cases (1998–2001). J Am Vet Med Assoc 224:1623–1629. doi:10.2460/javma.2004.224.1623
Schubach AO, Schubach TM, Barros MB (2005) Epidemic cat-transmitted sporotrichosis. N Engl J Med 353:1185–1186. doi:10.1056/NEJMc051680
Schubach A, Barros MB, Wanke B (2008) Epidemic sporotrichosis. Curr Opin Infect Dis 21:129–133. doi:10.1097/QCO.0b013e3282f44c52
Seifert KA (2009) Progress towards DNA barcoding of fungi. Mol Ecol Resour S1:83–89. doi:10.1111/j.1755-0998.2009.02635.x
Silva-Vergara ML, Camargo ZP, Silva PF, Abdalla MR, Sgarbieri RN, Rodrigues AM, dos Santos KC, Barata CH, Ferreira-Paim K (2012) Disseminated Sporothrix brasiliensis infection with endocardial and ocular involvement in an HIV-infected patient. AmJTrop Med Hyg 86:477–480. doi:10.4269/ajtmh.2012.11-0441
Summerbell RC (1993) The benomyl test as a fundamental diagnostic method for medical mycology. J Clin Microbiol 31:572–577
Tamura K (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Mol Biol Evol 9:678–687
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739. doi:10.1093/molbev/msr121
Toju H, Tanabe AS, Yamamoto S, Sato H (2012) High-Coverage ITS primers for the DNA-based identification of Ascomycetes and Basidiomycetes in environmental samples. PLoS One 7:e40863. doi:10.1371/journal.pone.0040863
Travassos LR, Lloyd KO (1980) Sporothrix schenckii and related species of Ceratocystis. Microbiol Rev 44:683–721
Verma S, Verma GK, Singh G, Kanga A, Shanker V, Singh D, Gupta P, Mokta K, Sharma V (2012) Sporotrichosis in sub-himalayan India. PLoS Negl Trop Dis 6:e1673. doi:10.1371/journal.pntd.0001673
Vismer HF, Hull PR (1997) Prevalence, epidemiology and geographical distribution of Sporothrix schenckii infections in Gauteng, South Africa. Mycopathologia 137:137–143. doi:10.1023/A:1006830131173
Wang XQ, Zhang Y, Chen YF, Han XP (2012) Clinical and pathological analysis of children’s cutaneous sporotrichosis in 56 cases. Chin J Dermatovenereol 26:405–406, 409 (in Chinese)
Watanabe S, Kawasaki M, Mochizuki T, Ishizaki H (2004) RFLP analysis of the internal transcribed spacer regions of Sporothrix schenckii. Jpn J Med Mycol 45:165–175
Zhang JD, Lin JP (2008) Clinical analysis of 316 cases of cutaneous sporotrichosis. Chin J Mycol 3:207–210 (in Chinese)
Zhou X, de Beer ZW, Wingfield MJ (2006) DNA sequence comparisons of Ophiostoma spp., including Ophiostoma aurorae sp. nov., associated with pine bark beetles in South Africa. Stud Mycol 55:269–277. doi:10.3114/sim.55.1.269
Zipfel RD, de Beer ZW, Jacobs K, Wingfield BD, Wingfield MJ (2006) Multi-gene phylogenies define Ceratocystiopsis and Grosmannia distinct from Ophiostoma. Stud Mycol 55:75–97. doi:10.3114/sim.55.1.75