Geographical comparison of genetic diversity in Asian landrace wheat (Triticum aestivum L.) germplasm based on high-molecular-weight glutenin subunits
Tóm tắt
Glutenin largely determines wheat bread baking quality. As high-molecular-weight glutenin subunit (HMW-GS), related to Glu-1 loci, determines wheat flour elasticity, it correlates strongly with bread-making quality. This study was aimed at clarifying genetic variations in bread-making characteristics between East and West Asian wheat landrace germplasms, by investigating HMW-GS allelic composition of 1068 wheat accessions. Herein, the accession number having reported HMW-GS pattern in previous studies was 855. However, the accession number with newly detected HMW-GS patterns was 114. These new HMW-GS patterns were classified into 4 types based on similarity. Eight Korean accessions with these four types were identified. Concerning landrace germplasm nature, 99 accessions showed heterogeneous patterns caused by seed mixture. The Glu-1 loci allelic variation analysis, revealed that the percentages of Glu-A1c (73.6%), Glu-B1b (60.2%), and Glu-D1a (68.5%) were highest at Glu-A1, Glu-B1, and Glu-D1 loci, respectively. The incidence of preferable alleles for bread baking was high in Chinese accessions. In bread-making quality evaluation using Glu-1 score, 24 among 35 accessions with full score were from China. The polymorphic information content index of each origin based on HMW glutenin subunit combination showed that West Asian and neighboring-regional landraces, excluding Afghanistan ones, were more diverse than East Asian landraces excluding Chinese ones. Cluster analysis based on Glu-1 allelic combination showed that many Korean, Japanese, and Afghan accessions were in the same group. However, many Chinese and other West Asian accessions were in the other group despite geographical distance.
Tài liệu tham khảo
Ahn JH, Lee SK, Park CS (2014) Evaluation of genetic variation at glutenin loci in Korean wheat landraces using allele-specific DNA markers. Plant Genet Resour C 12(3):353–356
Borlaug NE (1968) Wheat breeding and its impact on world food supply, vol 1335. Australian Academy Science, Canberra
D’Ovidio R, Masci S (2004) The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39:321–339
Dvorak J, Luo MC, Yang ZL, Zhang HB (1998) The structure of the Aegilops tauschii genepool and the evolution of hexaploid wheat. Theor Appl Genet 97(4):657–670
Fang J, Liu Y, Luo J, Wang Y, Shewry PR, He G (2009) Allelic variation and genetic diversity of high molecular weight glutenin subunit in Chinese endemic wheats (Triticum aestivum L.). Euphytica 166(2):177
Gianibelli MC, Larroque OR, MacRitchie F, Wrigley CW (2001) Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chem 78:635–646
Gregova E, Hermuth J, Kraic J, Dotlacil L (1999) Protein heterogeneity in European wheat landraces and obsolete cultivars. Additional information. Genet Resour Crop Evol 46:521–528
Gregova E, Hermuth J, Kraic J, Dotlacil L (2006) Protein heterogeneity in European wheat landraces and obsolete cultivars. Additional information. Genet Resour Crop Evol 53:867–871
Gupta RB, Shepherd KW (1990) Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. Theor Appl Genet 80(1):65–74
Hao CY, Dong YC, Wang LF, You GX, Zhang HN, Ge HM, Jia JZ, Zhang XY (2008) Genetic diversity and construction of core collection in Chinese wheat genetic resources. Chin Sci Bull 53:1518–1526
Harberd NP, Bartels D, Thompson RD (1986) DNA restriction-fragment variation in the gene family encoding high molecular weight(HMW) glutenin subunits of wheat. Biochem Genet 24:579–596
Hua C, Takata K, Yang-Fen Z, Ikeda TM, Yanaka M, Nagamine T, Fujimaki H (2005) Novel high molecular weight glutenin subunits at the Glu-D1 locus in wheat landraces from the Xinjiang District of China and relationship with winter habit. Breed Sci 55(4):459–463
Jackson EA, Morel MH, Sontag Strohm T, Branlard G, Metakovsky EV, Redaelli R (1996) Proposal for combining the classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat (Triticum aestivum L.). J Genet Breed 50:321–336
Khan K, Shewry PR (2009) Wheat: chemistry and technology. In: Shewry PR, D’Ovidio R, Lafiandra D, Jenkins JA, Mills ENC, Bekes F., (eds) Wheat grain protein. Am Assoc Cereal Chem (ed 4), St. Paul, pp 223–278
Kihara H (1944) Discovery of the DD-analyser, one of the ancestors of Triticum vulgare (abstr). Agric Hortic 19:889–890
Kim YM, Lee JY, Park CS, Kim HJ, Kim JH, Kim MS, Kim YT, Kang CS, Lim SH, Ha SH, Ahn SN (2013) Two-dimensional electrophoresis of high molecular weight glutenin subunits in Korean wheat cultivars. Korean J Breed Sci 45(3):240–252
Lagudah ES, Flood RG, Halloran GM (1987) Variation in high molecular weight glutenin subunits in landraces of hexaploid wheat from Afghnistan. Euphytica 36:3–9
Lee CW, Ito S, Watanabe M, Hoshino T (1995) Composition of high molecular weight-glutenin subunit in wheat cultivars. Miscv PUBL Tohoku Natl Agric Exp Stn 17:33–40
Lee SK, Choi YM, Hyun DY, Lee MC, Oh SJ, Hur OS, Ko HC, Jung YJ (2015) Evaluation of East Asian landrace wheat revealed by high molecular glutenin and maturity period. Korean J Breed Sci 47(3):264–275
Lei ZS, Gale KR, He ZH, Gianibelli C, Larroque O, Xia XC, Ma W (2006) Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat. J Cereal Sci 43(1):94–101
Liu K, Muse SV (2005) PowerMarker: integrated analysis environment for genetic marker data. BMC Bioinformatics 21:2128–2129
Liu Y, Xiong ZY, He YG, Shewry PR, He GY (2007) Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genet Resour Crop Evol 54(4):865–874
Liu S, Chao S, Anderson JA (2008) New DNA markers for high molecular weight glutenin subunits in wheat. Theor Appl Genet 118(1):177–183
Liu L, Ikeda TM, Branlard G, Peña RJ, Rogers WJ, Lerner SE, Appels R (2010) Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat. BMC Plant Biol 10(1):124
Ma W, Zhang W, Gale KR (2003) Multiplex PCR typing of high molecular weight glutenin alleles in wheat. Euphytica 134(1):51–60
McFadden ES, Sears ER (1946) The origin of Triticum spelta and its free-threshing hexaploid relatives. J Heredity 37(4):107–116
Moczulski M, Salmanowicz BP (2003) Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers. J Appl Genet 44(4):459–472
Nakamura H (2000) The high-molecular-weight glutenin subunit composition of Japanese hexaploid wheat landraces. Aust J Agric Res 51(6):673–677
Nakamura T, Vrinten P, Saito M, Konda M (2002) Rapid classification of partial waxy wheats using PCR-based markers. Genome 45(6):1150–1156
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci 70(12):3321–3323
Park CS, Shin SH, Kang CS, Kim KH (2012) Analysis of glutenin compositions in Korean wheat cultivar using SDS-PAGE and PCR. Korean J Breed Sci 44(3):245–257
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38(1):141–153
Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35.
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58(3):113–120
Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the High-molecular-weight subunits of wheat glutenin. Theor Appl Genet 60:229–236
Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and bread-making quality of British-grown wheat varieties. J Sci Food Agric 40:51–65
Ragupathy R, Naeem HA, Reimer E, Lukow OM, Sapirstein HD, Cloutier S (2008) Evolutionary origin of the segmental duplication encompassing the wheat Glu-B1 locus encoding the overexpressed Bx7 (Bx7OE) high molecular weight glutenin subunit. Theor Appl Genet 116(2):283–296
Rudd JC, Horsley RD, McKendry AL, Elias EM (2001) Host plant resistance genes for Fusarium head blight. Crop Sci 41(3):620–627
Shewry PR, Halford NG, Tatham AS (1992) High molecular weight subunits of wheat glutenin. J Cereal Sci 15:105–120
Singh A, Deveshwar J, Ahlawat A, Singh B (2007) Identification of novel variants of high molecular weight glutenin subunits in Indian bread wheat landraces. Cereal Res Commun 35(1):99–108
Tahir M, Pavoni A, Tucci GF, Turchetta T, Lafiandra D (1996) Detection and characterization of a glutenin subunit with unusually high Mr at the Glu-A1 locus in hexaploid wheat. Theor Appl Genet 92:353–360
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24(8):1596–1599
Terasawa Y, Kawahara T, Sasakuma T, Sasanuma T (2009) Evaluation of the genetic diversity of an Afghan wheat collection based on morphological variation, HMW glutenin subunit polymorphisms, and AFLP. Breed Sci 59(4):361–371
Terasawa Y, Takata K, Hirano H, Kato K, Kawahara T, Sasakuma T, Sasanuma T (2011) Genetic variation of high-molecular-weight glutenin subunit composition in Asian wheat. Genet Res Crop Evol 58(2):283–289
Wieser H (2007) Chemistry of glutenin proteins. Food Microbiol 24:115–119