Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

Central European Journal of Biology - Tập 9 - Trang 761-767 - 2014
Ivana Dokupilová1,2, Daniele Migliaro3, Daniel Mihálik1, Manna Crespan3, Ján Kraic1,4
1Plant Production Research Center Piešťany, Piešťany, Slovakia
2Slovak University of Technology in Bratislava, Bratislava, Slovakia
3Centro di Ricerca per la Viticoltura, Conegliano, Italy
4University of SS. Cyril and Methodius in Trnava, Trnava, Slovakia

Tóm tắt

Microsatellites were used as a very effective tool for genetic diversity analysis and characterization of 51 grapevine (Vitis vinifera L.) accessions from the national collection of genetic resources. Genetic diversity was relatively high, 8.91 alleles were detected per analysed microsatellite locus in average, and fifty-one accessions were distinguished into 45 groups. Distribution of recent Slovak cultivars across the dendrogram accented both their genetic diversity and the effectiveness of the national breeding program in maintaining genetic diversity and generating new genetic variants. Each cultivar was different from the others and twelve of them contained 77.6% of the total genetic diversity of the whole analysed set. Microsatellite patterns were also able to confirm parentage in selected Slovak cultivars. An unusual phenomenon of triallelism was also detected in one of the analysed accessions. The present study has initiated molecular characterization within the national grapevine genetic resource collection and their comparison with well-established international cultivars.

Tài liệu tham khảo

Grassi F., Labra M., Imazio S., Spada A., Sgorbati S., Scienza A., Sala F., Evidence of a secondary grapevine domestication centre detected by SSR analysis. Theor. Appl. Genet., 2003, 107, 1315–1320 Pelsy F., Molecular and cellular mechanisms of diversity within grapevine varieties. Heredity, 2010, 104, 331–340 Kraic J., Gregová E., Jomová K., Hudcovicová M., Microsatellite markers discriminating accessions within collection of plant genetic resources. Cell. Mol. Biol. Lett., 2002, 7, 745–751 Meneghetti S., Calò A., Bavaresco L., A strategy to investigate the intravarietal genetic variability in Vitis vinifera L. for clones and biotypes identification and to correlate molecular profiles with morphological traits or geographic origins. Mol. Biotechnol., 2011, DOI 10.1007/s12033-011-9475-6 Sefc M.K., Regner F., Turetschek E., Glössl J., Steinkellner H., Identication of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome, 1999, 42, 367–373 Merdinoglu D., Butterlin G., Bevilacqua L., Chiquet V., Adam-Blondon A.-F., Decroocq S., Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR. Mol. Breed., 2005, 15, 349–366 Riaz S., Tenscher A.C., Smith B.P., Ng D.A., Walker M.A., Use of SSR markers to assess identity, pedigree, and diversity of cultivated Muscadine grapes. J. Am. Soc. Hort. Sci., 2008, 133, 559–568 Loureiro M.D., Moreno-Sanz P., Suárez B., Clonal preselection of grapevine cultivars of the appellation “Cangas Quality Wine” (Asturias, Spain). Hort. Sci., 2011, 38, 71–80 Lacombe T., Audeguin L., Boselli M., Buchcetti B., Cabello F., Chatelet P., Crespan M., D’Onofrio C., Eiras Dias J., Ercisli S., Gardiman M., Grando M.S., Imazio S., Jandurová O., Jung A., Kiss E., Kozma P., Maul E., Maghradze D., Martinez M.C., Munoz G., Pátková J.K., Pejic I., Peterlunger E., Pitsoli D., Preiner D., Raimondi S., Regner F., Savin G., Savvides S., Schneider S., Spring J.L., Szoke A., Veres A., Boursiquot J.M., Bacilieri M., This P., Grapevine European catalogue: Towards a comprehensive list. Vitis, 2011, 50, 65–68 Bowers J.E., Dangl G.S., Meredith C.P., Development and characterization of additional microsatellite DNA markers for grape. Am. J. Enol. Viticult., 1999, 50, 243–246 Thomas M.R., Scott N.S., Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs). Theor. Appl. Genet., 1993, 86, 985–990 Sefc K.M., Regner F., Turetschek E., Glössl J.; Steinkellner H. Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome, 1999, 42, 367–373 Bowers J.E., Dangl G.S., Vignani R., Meredith C.P., Isolation and characterisation of the new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome, 1996, 39, 628–633 Crespan M., The parentage of Muscat of Hamburg. Vitis, 2003, 42, 193–197 Welter L.J., Göktürk-Baydar N., Akkurt M., Maul E., Eibach R., Töpfer R., Zyprian E. M., Genetic mapping and localization of quantitative trait loci affecting fungal disease resistance and leaf morphology in grapevine (Vitis vinifera L.). Mol. Breed., 2007, 20, 359–374 Nei M., Li W.-H., Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. USA, 1979, 76, 5269–5273 Crow J.F., Kimura M., Evolution in sexual and asexual populations. Am. Nat., 1965, 99, 439–450 Paetkau D., Calvert W., Stirling I., Strobeck C., Microsatellite analysis of population structure in Canadian polar bears. Mol. Ecol., 1995, 4, 347–354 Hammer Ø., Harper D.A.T., Ryan P.D., PAST: Paleontological statistics software package for education and data analysis. Palaeontol. Electron., 2001, 4, 1–9 Clayton T.M., Guest J.L., Urquhart A.J., Gill P.D., A genetic basis for anomalous band patterns encountered during DNA STR profiling. J. Forensic Sci., 2004, 49, 1–8 Hocquigny S., Pelsy F., Dumas V., Kindt S., Heloir M.-C., Merdinoglu D., Diversification within grapevine cultivars goes through chimeric states. Genome, 2004, 47, 579–589 Gago P., Santiago J.-L., Boso S., Alonso-Villaverde V., Grando M.S., Martínez M.C., Biodivesity and characterization of twenty-two Vitis vinifera L. cultivars in the nortwestern Iberian peninsula. Am. J. Enol. Vitic., 2009, 63, 293–301 Montaner C., Martín J.P., Casanova J., Martí C., Badía D., Cabello F., Ortiz J.M., Application of microsatellite markers for the characterization of ‘Parraleta’: an autochthonous Spanish grapevine cultivar. Sci. Hortic., 2004, 101, 343–347 Cipriani G., Marrazzo M.T., Di Gaspero G., Pfeiffer A., Morgante M., Testolin R., A set of microsatellite markers with long core repeat optimized for grape (Vitis spp.) genotyping. BMC Plant Biol., 2008, 8, 127 Cipriani G., Spadotto A., Jurman I., Di Gaspero G., Crespan M., Meneghetti S., Frare E., Vignani R., Cresti M., Morgante M., Pezzotti M., Pe E., Policriti A., Testolin R., The SSR-based molecular profile of 1005 grapevine (Vitis vinifera L.) accessions uncovers new synonymy and parentages, and reveals a large admixture amongst varieties of different geographic origin. Theor. Appl. Genet., 2010, 121, 1569–1585 Le Cunff L, Fournier-Level A., Laucou V., Vezzulli S., Lacombe T., Adam-Blondon A.-F., Boursiquot J.-M., This P. Construction of nested genetic core collections to optimize the exploitation of natural diversity in Vitis vinifera L. subsp. sativa. BMC Plant Biol., 2008, 8:31, doi:10.1186/1471-2229-8-31 Nookaraju A., Agrawal D.C., Genetic homogeneity of in vitro raised plants of grapevine cv. Crimson Seedless revealed by ISSR and microsatellite markers. S. Afr. J. Bot., 2012, 78, 302–306 Baleiras-Couto M.M., Eiras-Dias J.E., Detection and identification of grape varieties in must and wine using nuclear and chloroplast microsatellite markers. Anal. Chim. Acta, 2005, 563, 283–291 Cabezas J.A., Ibáñez J., Lijavetzky D., Vélez D., Bravo G., Rodríguez V., Carreño I., Jermakow A.M., Carreño J., Ruiz-García L., Thomas M.R., Martinez-Zapater J.M., A 48 SNP set for grapevine cultivar identification. BMC Plant Biol., 2011, 11, 153.