Genomic analysis of allelopathic response to low nitrogen and barnyardgrass competition in rice (Oryza sativa L.)

Plant Growth Regulation - Tập 61 - Trang 277-286 - 2010
Chang-Xun Fang1,2, Hai-Bin He1,2, Qing-Shui Wang1,2, Long Qiu1,2, Hai-Bin Wang1,2, Yue-E Zhuang1,2, Jun Xiong1,2, Wen-Xiong Lin1,2
1Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou, People’s Republic of China
2Agroecological Institute/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), Fuzhou, People’s Republic of China

Tóm tắt

To explore the molecular mechanism of allelopathic rice in response to low nitrogen (N) supply or accompanying weed stress, allelopathic rice PI 312777 and its counterpart Lemont were grown under low N supply or co-cultured with barnyardgrass [Echinochloa crus-galli (L.) Beauv.] in hydroponics. The suppression subtractive hybridization (SSH) technique was employed to isolate the up-regulated genes in the treated rice accession. The results indicated that the expression of the genes associated with N utilization was significantly up-regulated in allelopathic rice PI 312777, and the higher efficiency of N uptake and its utilization were also detected in PI 312777 than that in Lemont when the two rice accessions were exposed to low N supply. This result suggested that the allelopathic rice had higher ability to adapt to low N stress than its non-allelopathic counterpart. However, a different response was observed when the allelopathic rice was exposed to accompanying weed (barnyardgrass) co-cultured in full Hoagland solution (normal N supply). It showed that the expression of the genes associated with allelochemical synthesis and its detoxification were all up-regulated in the allelopathic rice when co-cultured with the target weed under normal N supply. The results suggested that the allelopathic rice should be a better competitor in the rice-weed co-culture system, which could be attributed to increasing de novo biosynthesis and detoxification of allelochemicals in rice, consequently resulting in enhanced allelopathic effect on the target and preventing the autotoxicity in this process. These findings suggested that the accompanying weed, barnyardgrass is not only the stressful factor, but also one of the triggers in activating allelopathy in rice. This implies that the allelopathic rice is sensible of the existing target in chemical communication.

Tài liệu tham khảo

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