Genome sequencing in microfabricated high-density picolitre reactors

Sanger, F., Nicklen, S. & Coulson, A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA 74, 5463–5467 (1977)

Prober, J. M. et al. A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides. Science 238, 336–341 (1987)

NIH News Release. NHGRI seeks next generation of sequencing technologies. 14 October 2004 http://www.genome.gov/12513210 .

Nyren, P., Pettersson, B. & Uhlen, M. Solid phase DNA minisequencing by an enzymatic luminometric inorganic pyrophosphate detection assay. Anal. Biochem. 208, 171–175 (1993)

Ronaghi, M. et al. Real-time DNA sequencing using detection of pyrophosphate release. Anal. Biochem. 242, 84–89 (1996)

Jacobson, K. B. et al. Applications of mass spectrometry to DNA sequencing. GATA 8, 223–229 (1991)

Bains, W. & Smith, G. C. A novel method for nucleic acid sequence determination. J. Theor. Biol. 135, 303–307 (1988)

Jett, J. H. et al. High-speed DNA sequencing: an approach based upon fluorescence detection of single molecules. Biomol. Struct. Dynam. 7, 301–309 (1989)

Tawfik, D. S. & Griffiths, A. D. Man-made cell-like compartments for molecular evolution. Nature Biotechnol. 16, 652–656 (1998)

Ghadessy, F. J., Ong, J. L. & Holliger, P. Directed evolution of polymerase function by compartmentalized self-replication. Proc. Natl Acad. Sci. USA 98, 4552–4557 (2001)

Dressman, D., Yan, H., Traverso, G., Kinzler, K. W. & Vogelstein, B. Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Proc. Natl Acad. Sci. USA 100, 8817–8822 (2003)

Ronaghi, M., Uhlen, M. & Nyren, P. A sequencing method based on real-time pyrophosphate. Science 281, 363–365 (1998)

Fraser, C. M. et al. The minimal gene complement of Mycoplasma genitalium. Science 270, 397–403 (1995)

Tettelin, H. et al. Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science 293, 498–506 (2001)

Leamon, J. H. et al. A massively parallel PicoTiterPlate based platform for discrete picoliter-scale polymerase chain reactions. Electrophoresis 24, 3769–3777 (2003)

Ronaghi, M. Pyrosequencing sheds light on DNA sequencing. Genome Res. 11, 3–11 (2001)

Ewing, B., Hillier, L., Wendl, M. C. & Green, P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 8, 175–185 (1998)

Moore, G. E. Cramming more components onto integrated circuits. Electronics 38(8) (1965)

Mehta, K., Rajesh, V. A. & Veeraswamy, S. FPGA implementation of VXIbus interface hardware. Biomed. Sci. Instrum. 29, 507–513 (1993)

Fagin, B., Watt, J. G. & Gross, R. A special-purpose processor for gene sequence analysis. Comput. Appl. Biosci. 9, 221–226 (1993)

Lander, E. S. & Waterman, M. S. Genomic mapping by fingerprinting random clones: a mathematical analysis. Genomics 2, 231–239 (1988)

Myers, E. W. Toward simplifying and accurately formulating fragment assembly. J Comput. Biol. 2, 275–290 (1995)

Ogawa, T. et al. Increased Productivity For Core Labs Using One Polymer and One Array Length for Multiple Applications. Poster P108-T. ABRF '05: Biomolecular Technologies: Discovery to Hypotheses (Savannah, Georgia, 5-8 February 2005)

also available as Applied Biosystems 3730x/DNA Analyzer Specification Sheet (2004).