Genome sequence of the potential probiotic eukaryote Saccharomyces cerevisiae KCCM 51299

3 Biotech - Tập 10 - Trang 1-9 - 2020
Junsang Hwang1,2, Ina Bang3, Donghyuk Kim3, Seung Chul Shin4, Young-Wook Chin5, Tae-Wan Kim5, Hyo Jin Kim1,2,3
1Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, Republic of Korea
2Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, Republic of Korea
3School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea
4Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, Republic of Korea
5Research Group of Traditional Food, Research Division of Strategic Food Technology, Korea Food Research Institute, Wanju-gun, Republic of Korea

Tóm tắt

Saccharomyces cerevisiae KCCM 51299, a potential probiotic yeast overproducing glutathione, has been isolated from among 272 yeast strains from the relatively safe Nuruk. The genome sequence of S. cerevisiae KCCM 51299 was analyzed and a near-complete genome (12 Mb) with 19 contigs was assembled after PacBio single-molecule real-time (SMRT) sequencing. The genome of S. cerevisiae KCCM 51299 was compared to the S. cerevisiae s288c reference genome. Additionally, genes involved in glutathione biosynthesis were identified, and the glutathione biosynthesis pathway was constructed in silico based on these genes. Furthermore, S. cerevisiae KCCM 51299 genes were compared with those in other yeast genomes. Finally, genome-scale in silico flux analysis was carried out, and a metabolic engineering strategy for glutathione biosynthesis was generated. These results provide useful information to further develop eukaryotic probiotics to overproduce glutathione.

Tài liệu tham khảo

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