So sánh bộ gen của các chủng Pichinde arenavirus có độc tính và không có độc tính

Archives of Virology - Tập 153 - Trang 1241-1250 - 2008
Shuiyun Lan1, Lisa McLay1, Judy Aronson2, Hinh Ly1, Yuying Liang1
1Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, USA
2Department of Pathology and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, USA

Tóm tắt

Một chủng virulent (P18) của virus Pichinde arenavirus gây ra một căn bệnh ở chuột lang tương tự như sốt Lassa ở người, trong khi một chủng avirulent (P2) của virus này được chứng minh là đã giảm độc tính ở các động vật nhiễm bệnh. Có giả thuyết rằng thành phần của bộ gen virus có thể ảnh hưởng đến mức độ độc tính trong một chủ thể nhiễm; tuy nhiên, trình tự đầy đủ của các bộ gen virus chưa được biết đến. Tại đây, chúng tôi cung cấp lần đầu tiên các trình tự bộ gen của các phân đoạn S và L cho cả hai chủng P2 và P18. So sánh trình tự xác định ba đột biến trong tiểu đơn vị GP1 của glycoprotein virus, một trong nucleoprotein NP, và năm trong protein polymerase RNA virus L. Những đột biến này, riêng lẻ hoặc kết hợp, có thể góp phần vào độc tính đã thu được của nhiễm virus Pichinde ở động vật. Ba thay đổi axit amin trong vùng biến đổi của tiểu đơn vị glycoprotein GP1 có thể ảnh hưởng đến việc xâm nhập của virus bằng cách thay đổi hoạt động gắn kết receptor của nó. Trong khi NP trước đây đã được chứng minh là điều chỉnh phản ứng miễn dịch của chủ thể đối với nhiễm virus, chúng tôi phát hiện rằng sự thay đổi R374 K trong protein này không ảnh hưởng đến chức năng của NP trong việc ức chế biểu hiện interferon-β. Bốn trong số năm thay đổi axit amin trong protein L xảy ra trong một vùng nhỏ của protein có thể góp phần vào độc tính của virus bằng cách tăng cường chức năng của nó trong tổng hợp RNA gen virus.

Từ khóa

#Pichinde arenavirus #virulent strain #avirulent strain #viral genome #glycoprotein #RNA polymerase

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