Genetics and mapping of seed coat impermeability in soybean using inter-specific populations
Tóm tắt
Seed coat impermeability (SCI) in soybean is associated with seed viability under storage and quality of processed products. Understanding genetics and identification of linked molecular markers would facilitate need-based utilization of seed coat impermeability. Two impermeable wild type (G. soja Sieb. and Zucc.) accessions viz. PI 424079 and PI 136620 were crossed with a permeable cultivated (G. max) variety JS335 to generate the mapping populations. Genetic analysis of the F1:2 and F2:3 seeds of the crosses indicated that SCI is controlled by a single gene/major QTL, and impermeability is dominant over permeability. Presence of seeds with intermediate permeability indicated role of some minor genes/QTLs. A set of 204 inter-specific recombinant inbred line (RILs) (F7) was used to map SCI with 207 SSR markers. Phenotyping through rapid imbibition approach (seed imbibition for 6 h), seven QTLs were mapped on chromosomes (Chrs.) 2, 5, 12, 13 and 16 in the seeds stored for 1–3 years, while through slow imbibition method (seed imbibition for 7 days), five QTLs were mapped on Chrs. 2, 9, 10 and 20. Phenotypic variation explained (PVE) by the QTLs ranged from 5.96 to 39.67%. Two major and stable QTLs viz., qScI-h2-1 and qScI-h2-2 that mapped in tandem on Chr.2 jointly explained 43.09–62.92% of the variations in impermeability. Seven minor QTLs identified here were novel and two (qScI-h5, and qScI-h16) were consistent. It is the first report of mapping impermeability using two imbibition approaches together in 200 plus inter-specific RILs in soybean. The study will pave the way for developing genotypes with restricted permeability, enhanced seed viability, and improved seeds quality.
Tài liệu tham khảo
Ai LJ, Chen Q, Yang CY, Yan L, Wang FM, Ge RC, Zhang MC (2018) Additive and epistatic QTL mapping for soybean hard seededness. Acta Agron Sin 44(6):852–858
Chandra S, Yadav RR, Poonia S, Yashpal DR, Rathod A, Kumar SKL, Talukdar A (2017) Seed coat permeability studies in wild and cultivated species of soybean. Int J Curr Micro Appl Sci 6(7):2358–2363
Chen JJ, Liu XX, Yu LL, Lu YP, Zhang ST, Zhang HC, Guan RX, Qiu LJ (2019) QTL mapping of hard seededness in wild soybean using BSA method. Sci Agri Sin 52(13):2208–2219
Egli DB, Tekrony DM, Heitholt JJ, Rupe J (2005) Air temperature during seed filling and soybean seed germination and vigor. Crop Sci 45:1329–1335
Hartwig EE, Potts HC (1987) Development and evaluation of impermeable seed coats for preserving soybean seed quality. Crop Sci 27:506–508
Hou FF, Thseng FS (1991) Studies on the flooding tolerance of soybean seed: varietal differences. Euphytica 57:l69-173
Hymowitz T, Singh RJ (1987) Taxonomy and speciation. In: Wilcox JR (ed) Soybeans: Improvement, Production and Uses, 2nd edn. American Society of Agronomy, Madison, WI, pp. 23–48
Jang SJ, Sato M, Sato K, Jitsuyama Y, Fujino K, Mori H (2015) A single nucleotide polymorphism in an endo-1, 4-β-glucanase gene controls seed coat permeability in soybean. PLoS One 10(6):e0128527
Kebede H, Smith JR, Ray JD (2014) Identification of a single gene for seed coat impermeability in soybean PI 594619. Theor Appl Genet 127(9):1991–2003
Keim P, Diers BW, Shoemaker RC (1990) Genetic analysis of soybean hard seededness with molecular markers. Theor Appl Genet 79:465–469
Kilen TC, Hartwig EE (1978) An inheritance study of impermeable seed in soybean. Field Crop Res 1:65–70
Kumar A, Chandra S, Talukdar A, Yadav RR, Saini M, Poonia S, Lal SK (2019) Genetic studies on seed coat permeability and viability in RILs derived from an inter-specific cross of soybean [Glycine max (L.) Merrill]. Indian J Genet 79(1):48–55
Kumawat G, Singh G, Gireesh C, Shivakumar M, Arya M, Agarwal DK, Husain SM (2015) Molecular characterization and genetic diversity analysis of soybean [Glycine max (L.) Merr.] germplasm accessions in India. Physiol Mol Biol Plants 21:101–107
Liu B, Fujita T, Yan Z, Sakamoto S, Xu D, Abe J (2007) QTL mapping of domestication-related traits in soybean (Glycine max L.). Ann Bot 100:1027–1038
Ma F, Cholewa E, Mohamed T, Peterson CA, Gijzen M (2004) Cracks in the palisade cuticle of soybean seed coats correlates with their permeability to water. Ann Bot 94:213–228
McCouch SR, Cho YG, Yano M, Paul EM, Blinstrub M, Morishima H, Kinoshita T (1997) Report of QTL nomenclature. Rice Genet Newsl 14:11–13
Meng L, Li H, Zhang L, Wang J (2015) QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J 3:269–283
Mullin WJ, Xu WL (2000) A study of the intervarietal differences of cotyledon and seed coat carbohydrates in soybean. Food Res Int 33:883–891
Palomeque L, Liu LJ, Li W, Hedges BR, Cober ER, Smid MP, Lukens L, Rajcan I (2010) Validation of mega-environment universal and specific QTL associated with seed yield and agronomic traits in soybeans. Theor Appl Genet 120:997–1003
Potts HC, Duangpatra J, Hairston WG, Delouche JC (1978) Some influences of hardseededness on soybean seed quality. Crop Sci 18:221–224
Radhika P, Gowda SJM, Kadoo NY, Mhase LB, Jarnadagni BM, Sainani MN, Chandra S, Gupta VS (2007) Development of an integrated intraspecific map of chickpea (Cicer arietinum L.) using two recombinant inbred line populations. Theor Appl Genet 115:209–216
Sakamoto S, Abe J, Kanazawa A, Shimamoto Y (2004) Marker-assisted analysis for hard seededness with isozyme and simple sequence repeat loci. Breed Sci 54:133–139
Saghai-Maroof MA, Soliman KM, Jergensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci 81:8014–8018
Singh RK, Raipuria RK, Bhatia VS, Rani A, Husain SM, Satyavathi CT, Chauhan GS, Mohapatra T (2008) Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean. Physio Mol Bio Plants 14(3):173–177
Starzinger EK, West SH (1982) An observation in the relationship of soybean seed colour to viability maintenance. Seed Sci Technol 10:301–305
Sun L, Miao Z, Cai C, Zhang D, Zhao M, Wu Y, Zhang X, Swarm SA, Zhou L, Zhang ZJ et al (2015) GmHs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean. Nature Genet 47(8):939–943
Wang J (2009) Inclusive composite interval mapping of quantitative trait genes. Acta Agron Sin 35:239–245
Watanabe S, Tajuddin T, Yamanaka N, Hayashi M, Harada K (2004) Analysis of QTLs for reproductive development and seed quality traits in soybean using recombinant inbred lines. Breed Sci 54:399–407
Yashpal DR, Rathod S, Chandra A, Kumar RRY, Talukdar A (2019) Deploying inter-specific recombinant inbred lines to map QTLs for yield-related traits in soybean. Indian J Genet 79(4):693–703
Zhang B, Chen P, Chen CY, Wang D, Shi A, Hou A, Ishibashi T (2008) Quantitative trait loci mapping of seed hardness in soybean. Crop Sci 48:1341–1349