Genetically encoding unnatural amino acids for cellular and neuronal studies

Nature Neuroscience - Tập 10 Số 8 - Trang 1063-1072 - 2007
Wenyuan Wang1, Jeffrey K. Takimoto, G.V. Louie, T.J. Baiga, Joseph P. Noel, Kuo‐Fen Lee, Paul A. Slesinger, Lei Wang
1Jack H. Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA.

Tóm tắt

Từ khóa


Tài liệu tham khảo

Callaway, E.M. A molecular and genetic arsenal for systems neuroscience. Trends Neurosci. 28, 196–201 (2005).

Giepmans, B.N., Adams, S.R., Ellisman, M.H. & Tsien, R.Y. The fluorescent toolbox for assessing protein location and function. Science 312, 217–224 (2006).

Gandhi, C.S. & Isacoff, E.Y. Shedding light on membrane proteins. Trends Neurosci. 28, 472–479 (2005).

Miyawaki, A. et al. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388, 882–887 (1997).

Miesenbock, G., De Angelis, D.A. & Rothman, J.E. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192–195 (1998).

Nagel, G. et al. Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses. Curr. Biol. 15, 2279–2284 (2005).

Wang, L. & Schultz, P.G. Expanding the genetic code. Angew. Chem. Int. Edn. Engl. 44, 34–66 (2004).

Nowak, M.W. et al. Nicotinic receptor binding site probed with unnatural amino acid incorporation in intact cells. Science 268, 439–442 (1995).

Lummis, S.C. et al. Cis-trans isomerization at a proline opens the pore of a neurotransmitter-gated ion channel. Nature 438, 248–252 (2005).

Lummis, S.C., Beene, D.L., Harrison, N.J., Lester, H.A. & Dougherty, D.A. A cation-pi binding interaction with a tyrosine in the binding site of the GABAC receptor. Chem. Biol. 12, 993–997 (2005).

Beene, D.L., Price, K.L., Lester, H.A., Dougherty, D.A. & Lummis, S.C. Tyrosine residues that control binding and gating in the 5-hydroxytryptamine3 receptor revealed by unnatural amino acid mutagenesis. J. Neurosci. 24, 9097–9104 (2004).

Wang, L., Brock, A., Herberich, B. & Schultz, P.G. Expanding the genetic code of Escherichia coli. Science 292, 498–500 (2001).

Johnston, D. et al. Dendritic potassium channels in hippocampal pyramidal neurons. J. Physiol. (Lond.) 525, 75–81 (2000).

Long, S.B., Campbell, E.B. & Mackinnon, R. Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science 309, 897–903 (2005).

Zhou, M., Morais-Cabral, J.H., Mann, S. & MacKinnon, R. Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors. Nature 411, 657–661 (2001).

Demo, S.D. & Yellen, G. The inactivation gate of the Shaker K+ channel behaves like an open-channel blocker. Neuron 7, 743–753 (1991).

Hoshi, T., Zagotta, W.N. & Aldrich, R.W. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science 250, 533–538 (1990).

Wang, L., Magliery, T.J., Liu, D.R. & Schultz, P.G. A new functional suppressor tRNA/aminoacyl-tRNA synthetase pair for the in vivo incorporation of unnatural amino acids into proteins. J. Am. Chem. Soc. 122, 5010–5011 (2000).

Wang, L. & Schultz, P.G. A general approach for the generation of orthogonal tRNAs. Chem. Biol. 8, 883–890 (2001).

Galli, G., Hofstetter, H. & Birnstiel, M.L. Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements. Nature 294, 626–631 (1981).

Myslinski, E., Ame, J.C., Krol, A. & Carbon, P. An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene. Nucleic Acids Res. 29, 2502–2509 (2001).

Edwards, H. & Schimmel, P. A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase. Mol. Cell. Biol. 10, 1633–1641 (1990).

Doctor, B.P. & Mudd, J.A. Species specificity of amino acid accepter ribonucleic acid and aminoacyl soluble ribonucleic acid synthetases. J. Biol. Chem. 238, 3677–3681 (1963).

Drabkin, H.J., Park, H.J. & Rajbhandary, U.L. Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene. Mol. Cell. Biol. 16, 907–913 (1996).

Summerer, D. et al. A genetically encoded fluorescent amino acid. Proc. Natl. Acad. Sci. USA 103, 9785–9789 (2006).

Murrell-Lagnado, R.D. & Aldrich, R.W. Interactions of amino terminal domains of Shaker K channels with a pore blocking site studied with synthetic peptides. J. Gen. Physiol. 102, 949–975 (1993).

Kondoh, S., Ishii, K., Nakamura, Y. & Taira, N. A mammalian transient type K+ channel, rat Kv1.4, has two potential domains that could produce rapid inactivation. J. Biol. Chem. 272, 19333–19338 (1997).

Antz, C. et al. NMR structure of inactivation gates from mammalian voltage-dependent potassium channels. Nature 385, 272–275 (1997).

Wissmann, R. et al. Solution structure and function of the “tandem inactivation domain” of the neuronal A-type potassium channel Kv1.4. J. Biol. Chem. 278, 16142–16150 (2003).

Sakamoto, K. et al. Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells. Nucleic Acids Res. 30, 4692–4699 (2002).

Paule, M.R. & White, R.J. Survey and summary: transcription by RNA polymerases I and III. Nucleic Acids Res. 28, 1283–1298 (2000).

Murphy, S., Di Liegro, C. & Melli, M. The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter. Cell 51, 81–87 (1987).

Yuan, Y. & Reddy, R. 5′ flanking sequences of human MRP/7–2 RNA gene are required and sufficient for the transcription by RNA polymerase III. Biochim. Biophys. Acta 1089, 33–39 (1991).

Muir, T.W. Semisynthesis of proteins by expressed protein ligation. Annu. Rev. Biochem. 72, 249–289 (2003).

Cornish, V.W., Mendel, D. & Schultz, P.G. Probing protein structure and function with an expanded genetic code. Angew. Chem. Int. Edn. Engl. 34, 621–633 (1995).

Wang, L., Xie, J. & Schultz, P.G. Expanding the genetic code. Annu. Rev. Biophys. Biomol. Struct. 35, 225–249 (2006).

Zufferey, R., Donello, J.E., Trono, D. & Hope, T.J. Woodchuck hepatitis virus post-transcriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J. Virol. 73, 2886–2892 (1999).

Chin, J.W. et al. An expanded eukaryotic genetic code. Science 301, 964–967 (2003).