Genetic variation of two weevil pests of sweet potato, Cylas formicarius (Coleoptera: Brentidae) and Euscepes postfasciatus (Coleoptera: Curculionidae), in Japan based on mitochondrial DNA

Applied Entomology and Zoology - Tập 56 - Trang 483-496 - 2021
Hiraku Yoshitake1,2, Makoto Arimoto3,4, Norihide Hinomoto3,5, Chi-Feng Lee6, Sheng-Shan Lu7, Sheryl A. Yap8, Analyn A. Cabras9
1Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan
2Institute for Plant Protection, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan
3Division of Applied Entomology and Zoology, Central Region Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan
4Research Division, Yokohama Plant Protection Station, Ministry of Agriculture, Forestry and Fisheries (MAFF), Yokohama, Japan
5Graduate School of Agriculture, Kyoto University, Kyoto, Japan
6Applied Zoology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan
7Division of Forest Protection, Taiwan Forestry Research Institute (TFRI), Taipei, Taiwan
8Institute of Weed Science, Entomology and Plant Pathology, College of Agriculture and Food Science, University of the Philippines Los Baños, Los Baños, Philippines
9Coleoptera Research Center, University of Mindanao, Davao, Philippines

Tóm tắt

We examined the genetic variation in the sequences of cytochrome c oxidase subunit I gene (DNA barcode region) and some other regions of mitochondrial DNA of sweet potato weevil, Cylas formicarius (Fabricius, 1798) (Coleoptera: Brentidae), and West Indian sweet potato weevil, Euscepes postfasciatus (Fairmaire, 1849) (Coleoptera: Curculionidae), in Japan. In the DNA barcode region of C. formicarius, 139 haplotypes were detected from 1705 individuals belonging to 46 geographical populations. In the maximum likelihood phylogenetic tree, haplotypes found in Japan were mainly divided into three clades. In the DNA barcode region of E. postfasciatus, two haplotypes were detected from 82 individuals belonging to eight geographical populations. Of those haplotypes, Hap 1 was detected from the Nansei Islands, Fiji, Samoa, and Tonga, while Hap 2 was detected from the Nansei Islands and Samoa. These results suggest that the Japanese populations of both the species were derived from several foreign countries. Based on the haplotype network analyses of some other gene regions, those regions may be useful for a more detailed estimation of the origin of an accidentally collected individual in non-distribution area in Japan.

Tài liệu tham khảo

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