Genetic variability in Russian wildrye (Psathyrostachys juncea) assessed by RAPD
Tóm tắt
To assess the genetic variation within and among accessions of Russian wildrye (Psathyrostachys juncea), 88 individuals of 11 accessions originated from geographically diverse locations were analyzed using randomly amplified polymorphic DNA (RAPD). Under the optimized condition, 56% of the 200 tested primers produced polymorphic RAPDs among accessions. High level RAPD marker variations existed both within and among accessions. About 56% and 44% of the amplified fragments were polymorphic within and among accessions, respectively. Accessions from neighboring geographical regions are more similar than those from distant regions. These results are comparable with those obtained from studies on chromosome C-banding and isozyme electrophoresis.
Tài liệu tham khảo
Byrum, J.R., P.M. Kimbirauskas, R.C. Showmaker & B.W. Diers, 1994. Identification of RAPD marker linked to the RPS4 gene in soybean [Glycine max (L.) Merr.]. Soyb Genet Newsl 20: 112-117.
Caetano-Anolles, G., B.J. Bassam & P.M. Gresshoff 1991. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnology 9: 553-556.
Chen, Q., R.H. Zhou, L.H. Li, X.Q. Li, X.M. Yang & Y.S. Dong, 1988. First intergeneric hybrid between Triticum aestivum and Psathyrostachys juncea. Kexue Tongbao (Beijing) 33: 2071-2074.
Chou, Q., M. Russell, D.E. Birch, J. Raymond & W. Bloch, 1992. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res 20: 1717-1723.
Comeau, A. & A. Plourde, 1987. Cell, tissue culture and intergeneric hybridization for barley yellow dwarf virus resistance in wheat. Can J Plant Pathol 9: 188-192.
Devos, K.M. & M.D. Gale, 1992. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84: 567-572.
Dewey, D.R., 1984. The genomic system of classification as a guide to intergeric hybridization with the perennial Triticeae. In: J.P. Gustafson (Ed.), Gene Manipulation in Plant Improvement, pp. 209-279, Plenim Press, New York.
Endo, T.R. & B.S. Gill, 1984. The heterochromatin distribution and genome evolution in diploid species of Elymus and Agropyron. Can J Genet Cytol 26: 669-678.
Faure, S., J.L. Noyer, J.P. Horry, P. Bakry & C. Lanaud, 1994. A molecular marker-based linkage map of diploid bananas (Musa acuminata). Theor Appl Genet 87: 517-526.
He, S., H. Ohm & S. Mackenzie, 1992. Detection of DNA sequence polymorphisms among wheat varieties. Theor Appl Genet 84: 573-578.
Heun, M., J.P. Murphy & T.D. Philips, 1994. A comparison of RAPD and isozyme analyses for determining the genetic relationships among Avena sterilis L. accessions. Theor Appl Genet 87: 689-696.
Huff, D.R., R. Peakall & P.E. Smouse, 1993. RAPD variation within and among nature populations of outcrossing buffalograss [Buchloe dactyloides (Nutt.) Engelm.]. Theor Appl Genet 86: 927-934.
Jain, A., S. Bhatia, S.S. Banga & S. Prakash, 1994. Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor Appl Genet 88: 116-122.
Jensen, K.B., Y.F. Zhang & D.R. Dewey, 1990. Mode of pollination of perennial species of the Triticeae in relation to genomically defined genera. Can J Plant Sci 70: 215-225.
Joshi, C.P. & H.T. Nguyen, 1993. Application of the random ampli-fied polymorphic DNA technique for the detection of polymorphism among wild and cultivated tetraploid wheat. Genome 36: 602-609.
Lassner, M.W., P. Peterson & J.I. Yoder, 1989. Simultaneous ampli-fication of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant Mow Bowel Rep 7: 116-128.
Linde-Laursen, I. & R. von Bothmer, 1986. Comparison of the karyotypes of Psathyrostachys juncea and P. huashanica (Poaceae) studied by banding techniques. Pl Syst Evol 151: 203-213.
M'Ribu, H.K. & K.W. Hilu, 1994. Detection of interspecific and intraspecific variatin Panicum millets through random amplified polymorphic DNA. Theor Appl Genet 88: 412-416.
Nelson, C.D.,W.L. Nance & R.L. Doudrick, 1993. A partial genetic linkage map of slash pine (Pinus elliottii Engelm. var. elliottii) based on random amplified polymorphic DNAs. Theor Appl Genet 87: 145-151.
Novy, R.G., C. Kobak, J. Goffreda & N. Vorsa, 1994. RAPDs identify varietal misclassification and regional divergence in cranberry (Vaccinium macrocarpon (Ait) Pursh]. Theor Appl Genet 88: 1004-1010.
Plourde, A., G. Fedak, C.A. St-Pierre & A. Comeau, 1990. A nover intergeneric hybrid in the Triticeae: Triticum aestivum x Psathyrostachys juncea. Theor Appl Genet 79: 45-48.
Quiros, C.F., J. Hu, P. This, A.M. Chevre & M. Delseny, 1991. Development and chromosomal localization of genomic-specific markers by polymerase chain reaction in Brassica. Theor Appl Genet 82: 627-632.
Rohlf, F.J., 1993. Numerical taxonomy and multivariate analysis system. Version 1.80. Exeter Software, setauket, N.Y.
Schachermayr, G., H. Siedler, M.D. Gale & H. Winzeler, 1994. Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor Appl Genet 88: 110-115.
Sobral, B.W. & R.J. Honeycutt, 1993. High output genetic mapping of polyploids using PCR-generated markers. Theor Appl Genet 86: 105-112.
Tinker, N.A., M.G. Fortin & D.E. Mather, 1993. Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl Genet 85: 976-984.
Transue, D.K., D.J. Fairbanks, L.R. Robison & W.R. Andersen, 1994. Species identification by RAPD analysis of grain amaranth genetic resources. Crop Sci 34: 1385-1389.
Vierling, R.A. & H.T. Nguyen, 1992. Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes. Theor Appl Genet 84: 835-838.
Wei, J.Z.,W.F. Campbell & R.R.C.Wang, 1995. Standard giemsa Cbandedg karyotype of Russian wildrye (Psathyrostachys juncea) and its use in identification of a deletion-translocation heterozygote. Genome 38: 1262-1270.
Wei, J.Z.,R.R.C. Wang, 1996. Allozyme variation in accessions of Russian wildrye. Crop Sci 36: 785-790.
Welsh, J. & M. McClelland, 1990. Fingerprinting genomes using arbitrary primers. Nucleic Acids Res 18: 7213-7218.
Wenger, R.H. & P.J. Nielsen, 1991. Reannealing of artificial heteroduplexes generated during PCR-mediated genetic isotyping. Trends Genet 6: 178.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18: 6531-6535.
Williams, J.G.K., M.K. Hanafey, J.A. Rafalski & S.V. Tingey, 1992. Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol 218: 704-740.
Wilkie, S.E., Isaac, P.G. & R.J. Slater, 1993. Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theor Appl Genet 86: 497-504.
Wu, L. & H. Lin, 1994. Identifying buffalograss [Buchloe dactyloides (Nutt.) Engelm.]. J Am Soc Hortic Sci 119: 126-130.