Genetic transformation of filamentous fungi

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Andrianopoulos A. and Hynes M. J. 1988 Cloning and analysis of the positively acting regulatory geneamdR fromAspergillus nidulans.Mol. Cell. Biol. 8: 3532–3541

Andrianopoulos A. and Hynes M. J. 1990 Sequence and functional analysis of the positively acting regulatory geneamd R fromAspergillus nidulans.Mol. Cell. Biol. 10: 3194–3203

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Davis M. A. and Hynes M. J. 1987 Complementation ofareA regulatory gene mutations ofAspergillus nidulans by the heterologous regulatory genenit-2 ofNeurospora crassa.Proc. Natl. Acad. Sci. USA 84: 3753–3757

Davis M. A., Cobbett C. S. and Hynes M. J. 1988 AnamdS-lacZ fusion for studying gene regulation inAspergillus.Gene 63: 199–212

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D’Enfert C. 1996 Selection of multiple disruption events inAspergillus fumigatus using the orotidine-5’-decarboxylase gene,pyrG, as a unique transformation marker.Curr. Genet. 30: 76–82

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Ferea T. L. and Bowman B. J. 1996 The vacuolar ATPase ofNeurospora crassa is indispensable: Inactivation of thevma-1 gene by repeat-induced point mutation.Genetics 143: 147–154

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Gems D. H. and Clutterbuck A. J. 1993 Co-transformation with autonomously-replicating helper plasmids facilitates gene cloning from anAspergillus nidulans gene library.Curr. Genet. 24: 520–524

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Hynes M. J., Corrick C. M. and King J. A. 1983 Isolation of genomic clones containing theamdS gene ofAspergillus nidulans and their use in the analysis of structural and regulatory mutations.Mol. Cell. Biol. 3: 1430–1439

Hynes M. J., Corrick C. M., Kelly J. M., and Littlejohn T. G. 1988 Identification of the sites of action for regulatory genes controlling theamdS gene ofAspergillus nidulans.Mol. Cell. Biol. 8: 2589–2596

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Judelson H. S., Coffey M. D., Arredondo F. R and Tyler B. M. 1993 Transformation of the oomycete pathogenPhytophthora megasperma f. sp.glycinea occurs by DNA integration into single or multiple chromosomes.Curr. Genet. 23: 211–218

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Katz M. E. and Hynes M. J. 1989 Isolation and analysis of the acetate regulatory gene,facB, fromAspergillus nidulans.Mol. Cell. Biol. 9: 5696–5701

Kelly J. M. and Hynes M. J. 1985 Transformation ofAspergillus niger by theamdS gene ofAspergillus nidulans.EMBO J. 4: 475–479

Kelly J. M. and Hynes M. J. 1987 Multiple copies of theamdS gene ofAspergillus nidulans cause titration oftrans-acting regulatory proteins.Curr. Genet. 12: 21–31

Kothe G. O. and Free S. J. 1996 Protocol for the electroporation of Neurospora spheroplasts.Fungal Genet. Newsl. 43:31–33

Kuspa A. and Loomis W. F. 1992 Tagging developmental genes inDictyostelium by restriction enzymemediated integration of plasmid DNA.Proc. Natl. Acad. Sci. USA 89: 8803–8807

Langdon T., Sheerins A., Ravagnani A., Gielkens M., Caddick M. X. and Arst H. N. 1995 Mutational analysis reveals dispensability of the N-terminal region of theAspergillus transcription factor mediating nitrogen metabolite repression.Mol. Microbiol. 17:877–888

Lee B. N. and Adams T. H. 1994 Overexpression offibA, an early regulator ofAspergillus asexual sporulation, leads to activation ofbrlA and premature initiation of development.Mol. Microbiol. 14:323–334

Littlejohn T. G. and Hynes M. J. 1992 Analysis of the site of action of theamdR product for regulation of theamdS gene ofAspergillus nidulans.Mol. Gen. Genet. 235:81–88

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Madi L., Ebbole D. J., White B. T. and Yanofsky C. 1994 Mutants ofNeurospora crassa that alter gene expression and conidia development.Proc. Natl. Acad. Sci. USA 91:6226–6230

Marathe S., Connerton I. F. and Fincham J. R. S. 1990 Duplication-induced mutation of a newNeurospora gene required for acetate utilization: Properties of the mutant and predicted amino acid sequence of the protein product.Mol. Cell. Biol. 10:2638–2644

May G. S., Gambino J., Weatherbee J. A. and Morris N. R. 1985 Identification and functional analysis of β-tubulin genes by site-specific integrative transformation inAspergillus nidulans.J. Cell. Biol. 101: 712–719

Miao V. P. W., Rountree M. R. and Selker E. U. 1995 Ectopic integration of transforming DNA is rare amongNeurospora transformants selected for gene replacement.Genetics 139:1533–1544

Miller B. L., Miller K. Y. and Timberlake W. E. 1985 Direct and indirect gene replacements inAspergillus nidulans.Mol. Cell. Biol. 5:1714–1721

Miller B. L., Miller K. Y. Roberti K. A. and Timberlake W. E. 1987 Position-dependent and-independent mechanisms regulate cell-specific expression of the SpoC1 gene cluster ofAspergillus nidulans.Mol. Cell. Biol. 7:427–434

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Papagiannopoulos P., Andrianopoulos A., Sharp J. A., Davis M. A. and Hynes M. J. 1996 Thehap C gene ofAspergillus nidulans is involved in the expression of CCAAT-containing promoters.Mol. Gen, Genet. 251:412–421

Parsons L. M., Davis M. A. and Hynes M. J. 1992 Identification of functional regions of the positively acting regulatory geneamdR fromAspergillus nidulans.Mol. Microbiol. 6:2999–3007

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Punt P. J., Greaves P. A., Kuyvenhoven A., van Deutekom J. C. T., Kinghorn J. R., Pouwels P. H. and van den Hondel C. A. M. J. J. 1991 A twin-reporter vector for simultaneous analysis of expression signals of divergently transcribed, contiguous genes in filamentous fungi.Gene 104:119–122

Punt P. J., Kuyvenhoven A. and van den Hondel C. A. M. J. J. 1995 A mini-promoterlacZ gene fusion for the analysis of fungal transcription control sequences.Gene 158:119–123

Randall T. A. and Reddy C. A. 1992 The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycetePhanerochaete chrysosporium.Curr. Genet. 21:255–260

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Roncero M. I. G., Jepsen L. P., Strøman P. and van Heeswijk R. 1989 Characterization of aleuA gene and an ARS element fromMucor circinelloides.Gene 84:335–343

Rothstein R. J. 1983 One-step gene disruption in yeast.Methods Enzymol. 101:202–211

Sanchez O. and Aguirre J. 1996 Efficient transformation ofAspergillus nidulans by electroporation of germinated conidia.Fungal Genet. Newsl. 43:48–51