Genetic transformation of Gentiana dahurica Fisch by Agrobacterium tumefaciens using zygotic embryo-derived callus

Springer Science and Business Media LLC - Tập 32 - Trang 629-634 - 2009
Shao-Bo Sun1, Lai-Sheng Meng2
1Gansu College of Traditional Chinese Medicine, Lanzhou, China
2School of Life Sciences, Tongji University, Shanghai, China

Tóm tắt

Gentiana dahurica Fisch is one of four important commercial Radix Gentianae stipulated by the Chinese Pharmacopoeia. We have established a rapid and effective regeneration system using zygotic embryo-derived callus of G.dahurica Fisch. Using this regeneration system, Agrobacteriumtumefaciens-mediated transformation of G. dahurica Fisch has been developed. Zygotic embryo-derived callus was infected with an A.tumefaciens strain (GV3130) harboring a pBI121 vector that contained an nptII selective marker gene. In a total of 60 zygotic embryo-derived calli assayed, frequency of calli with nptII gene PCR-positive transgenic plantlets is 5%. Transient glucuronidase (GUS) expression was observed from these transgenic plantlets. Frequency of GUS-positive transgenic plantlets (4/78) was approximately consistent with that of PCR assay (3/60). Our data indicate that we have successfully established a stable G. dahurica Fisch transformation system by A.tumefaciens. In general, this transformation system we have established might be useful for modifying physiological, medicinal and horticultural traits.

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