Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance
Tóm tắt
Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB), an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings.
Tài liệu tham khảo
Akbari G, Sanavy SAMM, Yousefzadeh S (2007) Effect of auxin and salt stress (NaCl) on seed germination of wheat cultivars (Triticum aestivum L.). Pak J Biol Sci 10:2557–2561
Cha-um S, Supaibulwatana K, Kirdmanee C (2007) Glycinebetaine accumulation, physiological characterizations and growth efficiency in salt-tolerant and salt-sensitive lines of Indica rice (Oryza sativa L. ssp. indica) in response to salt stress. J Agron Crop Sci 193:157–166
Chen S, Yang Y, Shi W, Ji Q, He F, Zhang Z, Cheng Z, Liu X, Xu M (2008) Badh2, encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acetyl-1-pyrroline, a major component in rice fragrance. Plant Cell 20:1850–1861
Cushman JC, Bohnert HJ (2000) Genomic approaches to plant stress tolerance. Curr Opin Plant Biol 3:117–124
De Vries S, Hoge H, Bisseling T (1988) Isolation of total and polysomal RNA from plant tissues. Plant Mol Biol Manual B6:1–13
Demiral T, Türkan I (2004) Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment? J Plant Physiol 161:1089–1100
Demiral T, Türkan I (2006) Exogenous glycinebetaine affects growth and proline accumulation and retards senescence in two rice cultivars under NaCl stress. Environ Exp Bot 56:72–79
Doyel JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Fitzgerald TL, Waters DLE, Henry RJ (2008) The effect of salt on betaine aldehyde dehydrogenase transcript levels and 2-acetyl-1-pyrroline concentration in fragrant and non-fragrant rice (Oryza sativa). Plant Sci 175:539–546
Guo BH, Zhang YM, Li HJ, Du LQL, Yin XC, Shou Y, Chu CC (2000) Transformation of wheat with a gene encoding for the betaine aldehyde dehydrogenase (BADH). Acta Bot Sin 42:279–283
Hasthanasombut S, Ntui V, Supaibulwatana K, Mii M, Nakamura I (2010) Expression of Indica rice OsBADH1 gene under salinity stress in transgenic tobacco. Plant Biotechnol Rep 4:75–83
Holmström KO, Somersalo S, Mandal A, Palva TE, Welin B (2000) Improved tolerance to salinity and low temperature in transgenic tobacco producing glycine betaine. J Exp Bot 51:177–185
Huang J, Hirji R, Adam L, Rozwadowski KL, Hammerlindl JK, Keller WA, Selvaraj G (2000) Genetic engineering of glycinebetaine production toward enhancing stress tolerance in plants: metabolic limitations. Plant Physiol 122:747–756
International Rice Genome Sequencing Project (2005) The map-based sequence of the rice genome. Nature 436:793–800
Khan MS, Yu X, Kikuchi A, Asahina M, Watanabe KN (2009) Genetic engineering of glycine betaine biosynthesis to enhance abiotic stress tolerance in plants. Plant Biotechnol J 26:125–134
Kumar S, Dhingra A, Daniell H (2004) Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance. Plant Physiol 136:2843–2854
Kura-Hotta M, Satoh K, Satoh S (1987) Relationship between photosynthesis and chlorophyll content during leaf senescence of rice seedlings. Plant Cell Physiol 28:1321–1329
Kusaba M (2004) RNA interference in crop plants. Curr Opin Biotech 15:139–143
Larher F, Jolivet Y, Briens M, Goas M (1982) Osmoregulation in higher plant halophytes: Organic nitrogen accumulation in glycine betaine and proline during the growth of Aster tripolium and Suaeda macrocarpa under saline conditions. Plant Sci Lett 24:201–210
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382
Luo D, Niu X, Wang Y, Zheng W, Chang L, Wang Q, Wei X, Yu G, Lu BR, Liu Y (2007) Functional defect at the rice choline monooxygenase locus from an unusual post-transcriptional processing is associated with the sequence elements of short-direct repeats. New Phytol 175:439–447
Mäkelä P, Kärkkäinen J, Somersalo S (2000) Effect of glycinebetaine on chloroplast ultrastructure, chlorophyll and protein content, and RuBPCO activities in tomato grown under drought or salinity. Biol Plant 43:471–475
McElroy D, Brettell RIS (1994) Foreign gene expression in transgenic cereals. Trends Biotechnol 12:62–68
McNeil SD, Rhodes D, Russell BL, Nuccio ML, Shachar-Hill Y, Hanson AD (2000) Metabolic modeling identifies key constraints on an engineered glycine betaine synthesis pathway in tobacco. Plant Physiol 124:153–162
Nakamura T, Yokota S, Muramoto Y, Tsutsui K, Oguri Y, Fukui K, Takabe T (1997) Expression of a betaine aldehyde dehydrogenase gene in rice, a glycinebetaine nonaccumulator, and possible localization of its protein in peroxisomes. Plant J 11:1115–1120
Niu X, Zheng W, Lu BR, Ren G, Huang W, Wang S, Liu J, Tang Z, Luo D, Wang Y, Liu Y (2007) An unusual posttranscriptional processing in two betaine aldehyde dehydrogenase loci of cereal crops directed by short, direct repeats in response to stress conditions. Plant Physiol 143:1929–1942
Noreen S, Ashraf M (2008) Alleviation of adverse effects of salt stress on sunflower (Helianthus annuus L.) by exogenous application of salicylic acid: growth and photosynthesis. Pak J Bot 40:1657–1663
Ramanjulu S, Bartels D (2002) Drought- and desiccation-induced modulation of gene expression in plants. Plant Cell Environ 25:141–151
Rathinasabapathi B, Gage DA, Mackill DJ, Hanson AD (1993) Cultivated and wild rices do not accumulate glycinebetaine due to deficiencies in two biosynthetic steps. Crop Sci 33:534–538
Sakamoto A, Valverde R, Alia, Chen THH, Murata N (2000) Transformation of Arabidopsis with the codA gene for choline oxidase enhances freezing tolerance of plants. Plant J 22:449–453
Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, New York
Shabala SN, Shabala SI, Martynenko AI, Babourina O, Newman IA (1998) Salinity effect on bioelectric activity, growth, Na+ accumulation and chlorophyll fluorescence of maize leaves: a comparative survey and prospects for screening. Aust J Plant Physiol 25:609–616
Shao G, Chen M, Wang W, Zhang G (2008) The effect of salinity pretreatment on Cd accumulation and Cd-induced stress in BADH-transgenic and nontransgenic rice seedlings. J Plant Growth Regul 27:205–210
Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3:217–223
Shirasawa K, Takabe T, Takabe T, Kishitani S (2006) Accumulation of glycinebetaine in rice plants that overexpress choline monooxygenase from spinach and evaluation of their tolerance to abiotic stress. Ann Bot 98:565–571
Su J, Hirji R, Zhang L, He C, Selvaraj G, Wu R (2006) Evaluation of the stress-inducible production of choline oxidase in transgenic rice as a strategy for producing the stress-protectant glycine betaine. J Exp Bot 57:1129–1135
Toki S (1997) Rapid and efficient Agrobacterium-mediated transformation in rice. Plant Mol Biol Rep 15:16–21
Weretilnyk EA, Hanson AD (1990) Molecular cloning of a plant betaine-aldehyde dehydrogenase, an enzyme implicated in adaptation to salinity and drought. Proc Natl Acad Sci USA 87:2745–2749
Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN (1982) Living with water stress: evolution of osmolyte systems. Science 217:1214–1222
Yang X, Liang Z, Lu C (2005) Genetic engineering of the biosynthesis of glycinebetaine enhances photosynthesis against high temperature stress in transgenic tobacco plants. Plant Physiol 138:2299–2309
Yang X, Liang Z, Wen X, Lu C (2008) Genetic engineering of the biosynthesis of glycinebetaine leads to increased tolerance of photosynthesis to salt stress in transgenic tobacco plants. Plant Mol Biol 66:73–86
Yoshida S (1972) Physiological aspects of grain yield. Ann Rev Plant Physiol 23:437–464
Zeng L, Shannon MC (2000) Effects of salinity on grain yield and yield components of rice at different seeding densities. Agron J 92:418–423
Zeng L, Shannon MC, Lesch SM (2001) Timing of salinity stress affects rice growth and yield components. Agric Water Manage 48:191–206
Zhang XY, Liang C, Wang GP, Luo Y, Wang W (2010) The protection of wheat plasma membrane under cold stress by glycine betaine overproduction. Biol Plant 54:83–88