Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance

Plant Cell, Tissue and Organ Culture - Tập 104 - Trang 79-89 - 2010
Supaporn Hasthanasombut1, Kanyaratt Supaibulwatana2, Masahiro Mii1, Ikuo Nakamura1
1Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, Matsudo City, Chiba, Japan
2Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand

Tóm tắt

Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB), an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings.

Tài liệu tham khảo

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