Genetic diversity in camelina germplasm as revealed by seed quality characteristics and RAPD polymorphism

Plant Breeding - Tập 124 Số 5 - Trang 446-453 - 2005
J. Vollmann1, H. Grausgruber1, Gertraud Stift2, V. Dryzhyruk3, T. Lelley2
1Department of Applied Plant Sciences and Plant Biotechnology, BOKU – University of Natural Resources and Applied Life Sciences Vienna, Gregor Mendel Str. 33, A-1180 Vienna, Austria, E-mail: [email protected]
2Department for Agrobiotechnology, IFA Tulln, BOKU – University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Str. 20, A-3430 Tulln, Austria
3Poltava Agricultural Academy, Skovoroda Str. 1/3, Poltava 36003, Ukraine

Tóm tắt

AbstractCamelina is an alternative oilseed crop species with limited information about the origin and diversity of available germplasm. Therefore, a set of 130 camelina accessions from a world collection was evaluated for oil content, protein content and 1000‐seed weight in field experiments grown in three macro‐environments in Austria. Based on phenotypic data, accessions were categorized into four groups with different seed characteristics using k‐means cluster analysis or principal component extraction. Subsequently, a representative set of 41 accessions was subjected to random amplified polymorphic DNA (RAPD) analysis. Of 24 primers, 15 were polymorphic producing a total of 30 marker loci. Genetic distance estimates between the 41 accessions were calculated, based both on RAPD polymorphism and on seed quality characteristics, and dendrograms were generated for comparison. Similarities were found between the two different clustering approaches, and grouping was partly in agreement with pedigree information or geographic origin. However, as the two estimates of diversity sampled different segments of the genome, i.e. regions coding for seed characteristics or phenotypically neutral genomic regions highlighted by discrete markers, the correlation between the two distance matrices was low.

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Plant Breeding

Tập 124 Số 5

446-453

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