Genetic control of dihydroflavonol 4‐reductase gene expression in Petunia hybrida

Plant Journal - Tập 6 Số 3 - Trang 295-310 - 1994
Henk Huits1, Anton G. M. Gerats2, Marja M. Kreike1, Joseph N. M. Mol1, Ronald Koes
1Department of Genetics, Vrije Universiteit, Institute for Molecular Biological Sciences, BioCentrum Amsterdam, De Boelelaan 1087, 1081HV, Amsterdam, The Netherlands
2Present address: Laboratorium voor Genetica, Rijksuniversiteit Gent, K.L. Ledeganckstraat 35, B9000 Gent, Belgium.

Tóm tắt

The functions of four loci (An1, An2, An4, and An6) which control pigmentation in flowers of Petunia hybrida have been characterized. Linkage‐analysis and molecular complementation experiments showed that the An6 locus contains the structural dfrA gene, encoding the enzyme dihydroflavonol 4‐reductase (DFR). Analysis of gus gene expression driven by the dfrA promoter in transgenic plants showed that the dfrA promoter is highly active in the flower corolla, the anthers and seeds and, at a lower level, in ovules and the flower stem. These data are discussed in relation to the expression of other pigmentation genes and the accumulation pattern of anthocyanins. The expression of the dfrA‐gus transgene was dependent on the genes an1 (in every tissue), an2 (in the flower limb only) and an4 (in anthers), demonstrating that these genes encode regulatory factors that control dfrA promoter activity.

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Plant Journal

Tập 6 Số 3

295-310

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