Genetic and biochemical analysis of brown eye mutation in Drosophila nasuta nasuta and Drosophila nasuta albomicans
Tóm tắt
By analyzing the progeny of crosses involving brown eye mutants and the wild types in two members of Drosophila nasuta subgroup namely D. n. nasuta and D. n. albomicans we could show that the mutant gene is recessive, located in the chromosome 2 and the alleles of this gene are present at different loci. A study of fitness in the eye color mutants in comparison with the wild types revealed that D. n. nasuta mutant has higher viability at both 25 ± 1°C and ambient temperatures; while D. n. albomicans mutant has faster rate of development only at 25 ± 1°C. Quantitative analysis of eye pigments in the mutants revealed that there is biosynthesis of both pteridines and xanthommatins unlike in bw/bw of D. melanogaster, where only xanthommatins are synthesized. In both the species, the pteridine quantities in mutants are similar; whereas xanthommatin quantity in
$$\user1{bw}_n \user1{/bw}_n$$
is 10 times higher than that of
$$\user1{bw}_a \user1{/bw}_a$$
. Further, the F1 progeny of intraspecific crosses (wild type X mutant) are found to have high amounts of pteridine, even when compared with parental wild type.
Tài liệu tham khảo
Batabyal, A.K. & N.S. Sidhu, 1972. Fertility study on different mutant strains of Drosophila melanogaster. Dros. Inf. Serv. 48: 48–49.
Bonnier, G., 1960. Experiments on hybrid superiority in Drosophila melanogaster. II. Rate of development from egg hatching to eclosion. Genetics 46: 86–91.
Clyde, M., 1977. The Drosophila nasuta species complex in South East Asia. Doctoral Dissertation, University of Queensland, Brisbane, Australia.
Duda, O., 1923. Die Orientalischen und Australian Drosophiliden-Arten des Ungarischen National-Museums zu Budapest. Ann. Mus. Nat. Hung. 20: 24–59.
Ferre, J., F.J. Silva, M.D. Real & J.L. Mensua, 1986. Pigment patterns in mutants affecting the biosynthesis of pteridines and xanthommatin in Drosophila melanogaster. Biochem. Genet. 24: 545–569.
Gai, P.G. & N.B. Krishnamurthy, 1983. Studies on the Drosophila fauna from Sampaje and Shiradi ghats, Karnataka, India. Dros. Inf. Serv. 59: 36–37.
Gowda, L.S., 1979. Cytotaxonomic and population genetical studies in Drosophila. Doctoral Dissertation, University of Mysore, Mysore, India.
Hegde, S.N. & M.S. Krishna, 1995. A spontaneous mutation in Drosophila bipectinata. Dros. Inf. Serv. 76: 80.
Kalisch, W.-E. & M. Zajonz, 1995. A technique to localize autosomal genes in Drosophila nasuta. Proc. Third Drosophila Meeting, University of Mysore, Mysore, India.
Krishna, M.S. & S.N. Hegde, 1998. A spontaneous mutation in Drosophila malerkotliana. Dros. Inf. Serv. 81: 211.
Lamb, C.G., 1914. Diptera, Heteroneuridae, Ortalidae, Trypetidae, Sepsidae, Micropezidae, Drosophilidae, Geomyzidae, Milichiidae of Seychelles. Trans. Linn. Soc. London. 16: 307–372.
Lewontin, R.C., 1955. The effects of population density and composition on viability in Drosophila melanogaster. Evolution 9: 27–41.
Lifschytz, E., 1974. Genes controlling chromosome activity; An X-linked mutation affecting Y-lampbrush loop activity in Drosophila hydei. Chromosoma 47: 415–427.
Lindsley, D.L. & G. Grell, 1972. Genetic variations of Drosophila melanogaster. Carnegie Institute Press, San Diego, California.
Lozovskaya, E.R. & M.B. Evengener, 1991. New mutants obtained by means of hybrid dysgenesis in Drosophila virilis. Dros. Inf. Serv. 70: 277–279.
Mather, W.B. & A.K. Pope, 1972. The nasuta complex in Taiwan. Dros. Inf. Serv. 49: 109–110.
Mohanty, S., S. Chatterjee & B. N. Singh, 1988. Variation in the expression of plexus mutation in D. ananassae. Dros. Inf. Serv. 67: 59–60.
Nirmala, S.S. & N.B. Krishnamurthy, 1972. Structural variability in natural population of Drosophila nasuta. Dros. Inf. Serv. 49: 72.
Nirmala, S.S. & N.B. Krishnamurthy, 1974. Cytogenetic studies on D. neonasuta-A member of the nasuta subgroup. J. Mysore Univ. India 26b: 162–167.
Prout, T., 1971. The relation between fitness components and population production in Drosophila. II. Population production. Genetics 68: 151–167.
Rajasekarasetty, M.R., S.R. Ramesh & N.B. Krishnamurthy, 1979. Analysis of inversions in natural populations of Drosophila nasuta nasuta. The Nucleus 22: 92–95.
Rajasekarasetty, M.R., S.R. Ramesh & N.B. Krishnamurthy, 1980. Interspecific chromosomal variation among few members of nasuta subgroup (Genus: Drosophila). Entomon 5: 1–12.
Ramachandra, N.B. & H.A. Ranganath, 1986a. Estimation of population fitness in two strains of Drosophila nasuta albomicans with and without supernumerary chromosomes. Ind. J. Exptl. Biol. 24: 137–141.
Ramachandra, N.B. & H.A. Ranganath, 1986b. The chromosomes of two Drosophila races: D. nasuta nasuta and D. nasuta albomicans. IV. Hybridization and karyotype repatterning. Chromosoma 93: 243–248.
Ramesh, S.R. & M.R. Rajasekarasetty, 1980. Studies on isozyme variations in a few members of Drosophila nasuta subgroup. Proc. Ind. Acad. Sci. (Anim. Sci.) 89: 197–213.
Ranganath, H.A. & N.B. Krishnamurthy, 1972. Preliminary survey of Drosophila in Biligirirangana Hills (Mysore, India). Dros. Inf. Serv. 48: 132.
Ranganath, H.A., M.R. Rajasekarasetty & N.B. Krishnamurthy, 1974. Evolutionary status of Indian Drosophila nasuta. Ind. J Heredity 6: 19–25.
Ranganath, H.A. & N.B. Krishnamurthy, 1975. Chromosomal polymorphism in Drosophila nasuta. III. Inverted gene arrangement in South Indian populations. J. Heredity 66: 90–96.
Ranganath, H.A. & K. Hägele, 1981. Karyotypic orthoselection in Drosophila. Naturwissenschaften 68: 527–528.
Ranganath, H.A. & K. Hägele, 1982. The chromosomes of two Drosophila races: D. n. nasuta and D. n. albomicans. I. Distribution and differentiation of heterochromatin. Chromosoma 85: 83–92.
Ranganath, H.A., E.R. Schmidt & K. Hägele, 1982. Satellite DNA of D. n. nasuta and D. n. albomicans: localization in polytene and metaphase chromosomes. Chromosoma 85: 361–368.
Real, M.D., J. Ferre & J.L. Mensua, 1985. Methods for the quantitative estimation of the red and brown pigments of D. melanogaster. Dros. Inf. Serv. 61: 198–199.
Ribo, G. & A. Prevosti, 1969. Viability gene frequency dependence in mutants of D. melanogaster. Dros. Inf. Serv. 44: 92.
Singh, B.N. & S. Sisodia, 1999. Mating propensity in Drosophila bipectinata under different sex-ratios and choice situations. Curr. Sci. 76: 222–225.
Shymala, B.V., P. Meera Rao & H.A. Ranganath, 1987. Collection data of Drosophila fauna at four different localities in South India. Dros. Inf. Serv. 66: 128–129.
Stursa, I., 1983. Fertility in a white eye mutant of D. subobscura. Dros. Inf. Serv. 59: 126.
Taylor, C.E., 1983. Mitochondrial selection by mutant strains of D. pseudobscura. Dros. Inf. Serv. 59: 126–128.
Wakahama, K.I., O. Kitagawa & O. Yamaguchi, 1971. Evolutionary and genetical studies on the Drosophila nasuta subgroup. Chromosomal polymorphism found in the natural population of D. albomicans. Dros. Inf. Serv. 46: 144.
Wakahama, K.I. & O. Kitagawa, 1972. Evolutionary and genetical studies on the Drosophila nasuta subgroup. II. Karyotypes of D. nasuta collected from Seychelles Islands. Jpn. J. Genet. 47: 129–131.
Wakahama, K.I. & O. Kitagawa, 1973. Evolutionary and genetical studies on the Drosophila nasuta subgroup. III. Eye color mutant ‘brown’. Jpn. J. Genet. 48: 452–453.
Wilson, F.D., M.R. Wheeler, M. Harget & M. Kambyselles, 1969. Cytogenetic relations in the Drosophila nasuta subgroup of the immigrans group of species. Studies in genetics. Univ. Texas Publ. 6918: 207–253.
Wilson, T.G. & K.B. Jacobson, 1977. Isolation and characterization of Pteridines from heads of Drosophila melanogaster by modified thin layer chromatography procedure. Biochem. Genet. 15: 307–319.
Ziegler, I., 1961. Genetic aspects of ommochrome and pterin pigments. Adv. Genet. 10: 349–403.
Ziegler, I. & R. Harmsen, 1969. The biology of pteridines in insects. Adv. Insect Physiol. 6: 143–201.